Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA)
Tri Ramadhani
Balai Penelitian dan Pengembangan Kesehatan Banjarnegara, Jalan Selamanik Nomor 16 A Banjarnegara, Jawa Tengah, Indonesia
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Public Health Veterinary

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Optimasi Analisis Melting Curve untuk Skrining Cepat dan Sensitif Mutasi V1016G pada Aedes aegypti Resisten Sintetik Piretroid dengan Reaksi Rantai Polimerase Spesifik Alel Dyah Widiastuti; Agustiningsih Agustiningsih; Ihda Zuyina Ratna Sari; Tri Ramadhani
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Volume 17 Nomor 2 Desember 2021
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/blb.v17i2.5283

Abstract

Detection of V1016G mutation is important for identifying the mechanism of synthetic pyrethroid resistance in Aedes aegypti population. The previous method has described an allele specific polymerase chain reaction (AS-PCR) using conventional PCR to detect the mutation. Although the method has great differentiating power and reproducibility, faster and more sensitive genotyping method is essential to accurately detect the mutation. This study evaluate the used of SYBR® Green real-time PCR and melting curve analysis (MCA) to identify the V1016G mutation. The collection of homozygous 1016G, heterozygous, and wild type (1016 V) mosquitoes DNA genome was extracted using genomic DNA mini kit. The SsoAdvanced™ Universal SYBR® Green Supermix was used to identify alleles by real-time PCR followed melting curve analysis of the amplicons. Melting curve analysis produced reproducible results for the loci tested. The melting temperature was reached at 78.5 oC for homozygous 1016G mosquito and at 86 oC for wild type mosquito. Meanwhile, the heterozigous mosquito revealed two peaks of melting temperature at both 78.5 oC and 86 oC. These easily interpretable and distinguishable melting curve results were consistent with AS-PCR results obtained for the same alleles. The described MCA application for screening V1016G mutation is fast and widely accessible also could be implemented under field conditions
Kajian Entomologi dalam Mendukung Pengendalian Malaria melalui Program Flying Health Care (FHC) di Kabupaten Yahukimo, Provinsi Papua Tri Ramadhani; Amirullah Amirullah; Rahmat Rahmat
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Volume 17 Nomor 2 Desember 2021
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/blb.v17i2.5303

Abstract

Flying Health Care is one of the programs of the Ministry of Health to accelerate the elimination of malaria in an integrated manner, especially in the Yahukimo Regency. The purpose of this activity is to describe the role of entomological aspects in malaria control efforts. The data includes cases of malaria, mosquitoes and Anopheles larvae, breeding place, and vector control efforts. Data was collected by observation, survey, and secondary data analysis. The results of the study show that malaria cases rank first out of 10 diseases in health facilities. Malaria cases were more common occurred at the age of 10-19 years, male and near to health facilities. Breeding places in the form of swamps and puddles in the environment around patients with water depths ranging from 5-30 cm. There is no entomological data at the district level or at Puskesmas Aplim. Ongoing vector control efforts are in the form of distributing insecticide-treated mosquito nets. Insecticide mosquito nets have not been fully distributed to the community, this is constrained by the existing infrastructure in health care facilities. Vector control efforts should be supported by the results of entomological surveys and it is important to do socialization on how to use and maintain mosquito nets at the time of mosquito net distribution.
Co-Authors Agustiningsih Agustiningsih Amirullah Amirullah Dyah Widiastuti Ihda Zuyina Ratna Sari Rahmat Rahmat