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All Journal TROPICAL GENETICS Tropical Genetics
Syazwan Saidin
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Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Medicine & Pharmacology

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Molecular assessment of native fish diversity in UNESCO heritage site, Tasik Raban, Malaysia using DNA barcoding: DNA Barcoding of Native Fishes in Tasik Raban, Malaysia Ramizah Abdull Rahman; Adibah Abu Bakar; Nurul Fizatul Nabilah Osman; Syazwan Saidin; Intan Faraha A. Ghani
Tropical Genetics Vol. 1 No. 2 (2021)
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (910.632 KB)

Abstract

Despite the fact that freshwater fishes have been studied for over a century, Malaysia's conservation status and management are still in their infancy. The poor progress of freshwater fish taxonomy and conservatory management is primarily due to a lack of interest and funding. There are still numerous unsolved taxonomic issues of freshwater fishes in Malaysia and this had a negative impact on national ichthyological research. As a result, the current research aims to aid the success of the molecular DNA barcode project, particularly in inland reservoirs such as Tasik Raban, Perak which is located in the UNESCO Heritage Site. The Cytochrome c oxidase subunit 1 (COI) marker was used in this project to ensure that native fishes were taxonomically and molecularly barcoded and ready to be accessible through internet databases. Such public references can aid in raising awareness about the management of local fish variety. Taxonomy and molecular characterization data can be utilized to plan future conservation efforts, particularly for depleted, unknown, or cryptic native species.
Development of Species-specific Cichla species eDNA Primers for Rapid Alien Invasive Species (AIS) Monitoring Nurul Fizatul Nabilah Osman; Adibah Abu Bakar; Syazwan Saidin; Ramizah Abdul Rahman; Intan Faraha Abd Ghani; Amirrudin A; Siti Azizah M. N.
Tropical Genetics Vol. 2 No. 1 (2022):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v2i1.21

Abstract

Peacock bass or the cichlids are known locally as top predator fishes which are invasive in Malaysia freshwater system. Detection probabilities for these fishes are typically low, especially using the conventional capture-survey method due to the fish’s behaviour of hiding beneath the water’s surface. Hence, environmental DNA (eDNA) monitoring is a relatively new non-invasive and rapid approach that can be used to assess the distribution of these invasive fishes. Here, we report the strategy i) to develop small fragment (280- 400bp) specific-specific primers for three selected invasive Cichla species namely, C. kelberi, C. ocellaris, and C. monoculus based on mitochondrial DNA (mtDNA) COI gene sequences, ii) how high quality of DNA and e-DNA should be extracted and iii) how to validate primers for specificity, (iv) developed and tested a new quantitative PCR (qPCR) assay to detect the presence of C. kelberi, and C. ocellaris, environmental DNA (eDNA) in water samples. Current research showed that the cytochrome oxidase I (COI) gene can be used to developed species- specific primers for selected species. However, several primer-designed parameters need to be adjusted to improve the specificity of primers. These were discussed in detail with future recommendations for the researcher’s references. Moreover, we found the isolation of e-DNA needs to be done within 22 days of capture to prevent false-negative results. This data can be easily applied in any fish genomic-based lab with the low-cost setting as only AGE (Agarose Gel Electrophoresis) analysis was needed for validation procedures. Overall, e-DNA analysis with species- specific primers represents new monitoring tool in detection of Aquatic Invasive Species (AIS) for management and conservation purpose.
Molecular assessment of native fish diversity in UNESCO heritage site, Tasik Raban, Malaysia using DNA barcoding: DNA Barcoding of Native Fishes in Tasik Raban, Malaysia Ramizah Abdull Rahman; Adibah Abu Bakar; Nurul Fizatul Nabilah Osman; Syazwan Saidin; Intan Faraha A. Ghani
Tropical Genetics Vol. 1 No. 2 (2021)
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Despite the fact that freshwater fishes have been studied for over a century, Malaysia's conservation status and management are still in their infancy. The poor progress of freshwater fish taxonomy and conservatory management is primarily due to a lack of interest and funding. There are still numerous unsolved taxonomic issues of freshwater fishes in Malaysia and this had a negative impact on national ichthyological research. As a result, the current research aims to aid the success of the molecular DNA barcode project, particularly in inland reservoirs such as Tasik Raban, Perak which is located in the UNESCO Heritage Site. The Cytochrome c oxidase subunit 1 (COI) marker was used in this project to ensure that native fishes were taxonomically and molecularly barcoded and ready to be accessible through internet databases. Such public references can aid in raising awareness about the management of local fish variety. Taxonomy and molecular characterization data can be utilized to plan future conservation efforts, particularly for depleted, unknown, or cryptic native species.
Co-Authors Adibah Abu Bakar Adibah Abu Bakar Amirrudin A Intan Faraha A. Ghani Intan Faraha Abd Ghani Nurul Fizatul Nabilah Osman Nurul Fizatul Nabilah Osman Ramizah Abdul Rahman Ramizah Abdull Rahman Siti Azizah M. N.