Laksmita Prima Santi
Indonesian Research Institute for Biotechnology and Bioindustry

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Pemanfaatan Biochar Asal Cangkang Kelapa Sawit untuk Meningkatkan Serapan Hara dan Sekuestrasi Karbon pada Media Tanah Lithic Hapludults di Pembibitan Kelapa Sawit Laksmita Prima Santi
Jurnal Tanah dan Iklim (Indonesian Soil and Climate Journal) Vol 41, No 1 (2017)
Publisher : Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jti.v41n1.2017.9-16

Abstract

Abstrak: Pemanfaatan biochar asal cangkang kelapa sawit sebagai pembenah tanah memiliki potensi yang baik dalam upaya pengembangan perkebunan kelapa sawit di lahan sub optimal secara berkelanjutan. Kegiatan penelitian ini dilaksanakan untuk mempelajari pengaruh tiga tingkat dosis biochar (50; 100; dan 150 gram) yang dikombinasikan dengan 75% dosis pupuk NPK-Mg per bibit terhadap pertumbuhan bibit kelapa sawit, serapan hara, dan sekuestrasi karbon pada tahap pembibitan utama. Kegiatan lapang dilaksanakan di PT Astra Agro Lestari Tbk, Kalimantan Tengah pada bulan Maret 2015 - April 2016. Penelitian disusun dalam rancangan acak kelompok (RAK) dengan lima perlakuan dan dua puluh ulangan yaitu: (i) 100% dosis standar NPK-Mg per bibit (kontrol); (ii) 75% dosis standar NPK-Mg per bibit; (iii) 75% dosis standar NPK-Mg + 50 g biochar per bibit; (iv) 75% dosis standar NPK-Mg + 100 g biochar per bibit; dan (v) 75% dosis standar NPK-Mg + 150 g biochar per bibit. Pertumbuhan terbaik bibit kelapa sawit yang ditanam dalam media jenis tanah Lithic Hapludults diperoleh dari perlakuan 75% dosis pupuk NPK-Mg yang dikombinasikan dengan 150 gram biochar per bibit. Perlakuan ini berturut-turut meningkatkan tinggi (33,3%); jumlah daun (36,2%); diameter batang (28,9%); panjang dan lebar daun (22,6 dan 33,3%), bobot kering bibit kelapa sawit, khususnya bagian akar (65,2%), kapasitas tukar kation (17,2%), dan C-organik (26,9%). Hasil analisis terhadap perlakuan ini juga menunjukkan serapan hara N daun bibit kelapa sawit yang paling tinggi serta kadar hara P dan K yang tidak berbeda jika dibandingkan dengan perlakuan 100% dosis pupuk NPK-Mg. Atas dasar hasil tersebut maka 75% dosis pupuk NPK-Mg yang dikombinasikan dengan 150 gram biochar per bibit dapat direkomendasikan untuk digunakan pada bibit kelapa sawit.Abstract. The potential of biochar derived from oil palm kernel shell to be used as a soil amendment seems promising for sustaining oil palm plantation in marginal land. This study was conducted to investigate the effects of three biochar dosages (50; 100; and 150 gram) in combination with 75% NPK-Mg dosages per seedling on the growth of oil palm seedling, nutrient uptake and carbon sequestration in the nursery stage. A field experiment was conducted at PT Astra Agro Lestari Tbk, Central Kalimantan in March 2015- April 2016. Experiment was arranged in a completely randomized block design (CRBD) with five treatments and twenty replications i.e: (i) 100% standard dosage NPK-Mg per seed (control); (ii) 75% standard dosage NPK-Mg per seed; (iii) 75% standard dosage NPK-Mg + 50 g biochar per seed; (iv) 75% standard dosage NPK-Mg + 100 g biochar per seed; and (v) 75% standard dosage NPK-Mg + 150 g biochar per seed. The best vegetative growth performance of oil palm seedlings in Lithic Hapludults soil was shown by the application of 75% standard dosage of NPK-Mg fertilizers in combination with 150 gram biochar per seed. This treatment increased height (33.9%); leaf number (36.2%); stem diameter (28.9%); length and width of the leaf (22.6 and 33.3%), dry weight of oil palm seedlings, especially roots (65.2%), CEC (17.2%), and organic C (26.9%). This treatment also showed the highest absorption of N in leaves of oil palm seedlings, but no differences were observed for P and K uptakes compared to the treatment of 100% dosage of NPK-Mg fertilizer. On the basis of these results, the 75% dosage of NPK-Mg combined with 150 g of biochar per seed can be recommended for use on oil palm seedlings.
Application of bio-silicic acid to improve yield and fertilizer efficiency of paddy on tidal swamp land Donny Nugroho KALBUADI; Laksmita Prima SANTI; Didiek Hadjar GOENADI; Junita BARUS
E-Journal Menara Perkebunan Vol 88, No 2 (2020): Oktober,2020
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i2.378

Abstract

The soils in tidal swamp land as new development area for rice in Indonesia have a considerably low available silica (Si) because very acid condition and no Si fertilization. Therefore, increasing the productivity of rice as Si accumulator plant, in tidal swamp land requires silica fertilizer. This research presented the effect of applications of silicon in the form of orthosilicic acid (H4SiO4) enriched with selected Sisolubilizing fungi, formulated as 4-gram tableted Si fertilizer (BioSilAc) on tidal swamp land soil to improve yield and reduce chemical fertilizer dosage for rice. Field experiment was conducted in Ketapang subdistrict, South Lampung by using a Randomized Block Design (RBD) with six treatments and four replicates. The treatments consisted of (ha-1 season-1): 100% NPK recommended dosage (P1); 100% NPK + 80 BioSilAc tablets (P2); 75% NPK + 80 BioSilAc tablets (P3); 75% NPK + 100 BioSilAc tablets (P4); 50% NPK + 120 BioSilAc tablets (P5); and control (P6). The results showed that P2 treatment increased the highest rice yield in dried harvested grain by 7.6% or equivalent to 500 kg ha-1 compared to P1 treatment. The highest fertilization efficiency can be achieved in P4 treatment which means the NPK recommended dosage can be reduced 25% by adding 100 BioSilAc tablets ha-1 season-1 indicated by insignificantly different productivity compared to that of P1. The Revenue/Cost (R/C) values showed that P2 treatment was economically feasible fertilization practices.
Physiological responses of bio-silica-treated oil palm seedlings to drought stress (Tanggap fisiologi bibit kelapa sawit yang diberi bio-silika terhadap cekaman kekeringan) Dian mutiara AMANAH; . NURHAIMI-HARIS; Laksmita Prima SANTI
E-Journal Menara Perkebunan Vol 87, No 1 (2019): April, 2019
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (651.817 KB) | DOI: 10.22302/iribb.jur.mp.v87i1.306

Abstract

Silica (Si) in the form of soluble silicic acid [H4SiO4] was an element that makes plants more resistant to drought stress through biochemical or molecular processes and contributing to growth stimulation under biotic and abiotic stress conditions. The objective of this study was to determine the response of oil palm seedlings to drought stress by the bio-Si application. The experiment was arranged in complete random design (CRD) with ten replicates.  Bio-Si was developed in solid and liquid forms with a dissolved Si content at least 10% (w/v). The eight combinations of solid bio-Si application per seedling were: (i) blank (without fertilizers), (ii) 5 g NPK 15-15-15, (iii) 5 g NPK 15-15-15 + 109cfu of Si-solubilizing microbes (SSM), (iv-viii) 5 g NPK 15-15-15 + 2.5; 5.0; 7.5; 10 g bio-Si; and 5 g Na2SiO3.  On the other hand, liquid bio-Si application per seedling were: (i) blank (without fertilizers), (ii) 5 g NPK 15-15-15, (iii) 5 g NPK 15-15-15 + 109cfu of SSM, (iv-viii) 5 g NPK 15-15-15 + 25 mL; 50 mL; 75 mL; 100 mL bio-Si; and 50 mL Na2SiO3. Drought stress tolerance was analyzed by using proline concentration, nitrate reductase activity (NRA), chlorophyll content, and stomatal closure in the leave of oil palm seedlings. Based on the physiological response, this research indicates that bio-Si application could induce seedling tolerance to drought stress. The bio-Si treatments gave a positive response of proline concentration, nitrate reductase activity (NRA), chlorophyll content, and stomatal closure. The doses of 5 g NPK 15-15-15 + 7.5 g solid bio-Si and 5 g NPK 15-15-15 + 75 mL liquid bio-Si per seedling were a recommended to increase oil palm seedlings tolerance to drought stress.[Key words: bio-Si, chlorophyll, nitrate reductase activity, Si-solubilizing microbes]. AbstrakSilika (Si) dalam bentuk terlarut asam silikat [H4SiO4]merupakan unsur yang dapat menyebabkan tanaman lebih tahan terhadap cekaman kekeringan melalui proses biokimia atau molekuler dan menstimulasi pertumbuhan dalam kondisi cekaman biotik dan abiotik. Tujuan dari penelitian ini adalah mengetahui respons fisiologi bibit kelapa sawit yang diberi bio-Si terhadap cekaman kekeringan. Penelitian didesain dengan rancangan acak lengkap (RAL) dan sepuluh ulangan. Bio-Si dikembangkan dalam bentuk padat dan cair dengan kadar Si terlarut minimal 10 % (b/v). Delapan aplikasi bio-Si padat per bibit adalah: (i) blanko (tanpa pupuk), (ii) 5 g NPK 15-15-15, (iii) 5 g NPK 15-15-15 + 109cfu mikrob pelarut silika, (iv-viii) 5 g NPK 15-15-15 + 2,5 g; 5,0 g; 7,5 g; 10 g bio-Si, dan 5 g Na2SiO3. Sementara untuk aplikasi bio-Si cair per bibit adalah: (i) blanko (tanpa pupuk), (ii) 5 g NPK 15-15-15, (iii) 5 g NPK  15-15-15 + 109cfu mikroorganisme pelarut silika (MPS), (iv-viii) 5 g NPK 15-15-15 + 25 ml; 50 ml; 75 ml; dan 100 mLbio-Si, dan 50 ml Na2SiO3. Pengamatan yang dilakukan meliputi analisis prolin, aktivitas nitrat reduktase (ANR), kandungan klorofil, serta morfologi stomata pada daun bibit kelapa sawit. Berdasarkan data fisiologi yang diperoleh dari kegiatan penelitian ini, aplikasi bio-Si dapat meningkatkan ketahanan bibit kelapa sawit terhadap cekaman kekeringan. Perlakuan bio-Si memberikan respon positif terhadap konsentrasi prolin,aktivitas nitrat reduktase (ANR), kandungan klorofil, serta morfologi stomata.Dosis 5 g NPK 15-15-15 + 7,5 g bio-Si padat dan 5 g NPK 15-15-15 + 75 mLbio-Si cair dapat direkomendasikan untuk meningkatkan ketahanan bibit kelapa sawit terhadap cekaman kekeringan.  [Kata kunci: bio-Si, klorofil, aktivitas nitrat reduktase, mikroorganisme pelarut silika].
Physiological responses of bio-silica-treated oil palm seedlings to drought stress (Tanggap fisiologi bibit kelapa sawit yang diberi bio-silika terhadap cekaman kekeringan) Dian mutiara AMANAH; . NURHAIMI-HARIS; Laksmita Prima SANTI
Menara Perkebunan Vol. 87 No. 1 (2019): 87 (1), 2019
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v87i1.306

Abstract

Silica (Si) in the form of soluble silicic acid [H4SiO4] was an element that makes plants more resistant to drought stress through biochemical or molecular processes and contributing to growth stimulation under biotic and abiotic stress conditions. The objective of this study was to determine the response of oil palm seedlings to drought stress by the bio-Si application. The experiment was arranged in complete random design (CRD) with ten replicates.  Bio-Si was developed in solid and liquid forms with a dissolved Si content at least 10% (w/v). The eight combinations of solid bio-Si application per seedling were: (i) blank (without fertilizers), (ii) 5 g NPK 15-15-15, (iii) 5 g NPK 15-15-15 + 109cfu of Si-solubilizing microbes (SSM), (iv-viii) 5 g NPK 15-15-15 + 2.5; 5.0; 7.5; 10 g bio-Si; and 5 g Na2SiO3.  On the other hand, liquid bio-Si application per seedling were: (i) blank (without fertilizers), (ii) 5 g NPK 15-15-15, (iii) 5 g NPK 15-15-15 + 109cfu of SSM, (iv-viii) 5 g NPK 15-15-15 + 25 mL; 50 mL; 75 mL; 100 mL bio-Si; and 50 mL Na2SiO3. Drought stress tolerance was analyzed by using proline concentration, nitrate reductase activity (NRA), chlorophyll content, and stomatal closure in the leave of oil palm seedlings. Based on the physiological response, this research indicates that bio-Si application could induce seedling tolerance to drought stress. The bio-Si treatments gave a positive response of proline concentration, nitrate reductase activity (NRA), chlorophyll content, and stomatal closure. The doses of 5 g NPK 15-15-15 + 7.5 g solid bio-Si and 5 g NPK 15-15-15 + 75 mL liquid bio-Si per seedling were a recommended to increase oil palm seedlings tolerance to drought stress.[Key words: bio-Si, chlorophyll, nitrate reductase activity, Si-solubilizing microbes]. AbstrakSilika (Si) dalam bentuk terlarut asam silikat [H4SiO4]merupakan unsur yang dapat menyebabkan tanaman lebih tahan terhadap cekaman kekeringan melalui proses biokimia atau molekuler dan menstimulasi pertumbuhan dalam kondisi cekaman biotik dan abiotik. Tujuan dari penelitian ini adalah mengetahui respons fisiologi bibit kelapa sawit yang diberi bio-Si terhadap cekaman kekeringan. Penelitian didesain dengan rancangan acak lengkap (RAL) dan sepuluh ulangan. Bio-Si dikembangkan dalam bentuk padat dan cair dengan kadar Si terlarut minimal 10 % (b/v). Delapan aplikasi bio-Si padat per bibit adalah: (i) blanko (tanpa pupuk), (ii) 5 g NPK 15-15-15, (iii) 5 g NPK 15-15-15 + 109cfu mikrob pelarut silika, (iv-viii) 5 g NPK 15-15-15 + 2,5 g; 5,0 g; 7,5 g; 10 g bio-Si, dan 5 g Na2SiO3. Sementara untuk aplikasi bio-Si cair per bibit adalah: (i) blanko (tanpa pupuk), (ii) 5 g NPK 15-15-15, (iii) 5 g NPK  15-15-15 + 109cfu mikroorganisme pelarut silika (MPS), (iv-viii) 5 g NPK 15-15-15 + 25 ml; 50 ml; 75 ml; dan 100 mLbio-Si, dan 50 ml Na2SiO3. Pengamatan yang dilakukan meliputi analisis prolin, aktivitas nitrat reduktase (ANR), kandungan klorofil, serta morfologi stomata pada daun bibit kelapa sawit. Berdasarkan data fisiologi yang diperoleh dari kegiatan penelitian ini, aplikasi bio-Si dapat meningkatkan ketahanan bibit kelapa sawit terhadap cekaman kekeringan. Perlakuan bio-Si memberikan respon positif terhadap konsentrasi prolin,aktivitas nitrat reduktase (ANR), kandungan klorofil, serta morfologi stomata.Dosis 5 g NPK 15-15-15 + 7,5 g bio-Si padat dan 5 g NPK 15-15-15 + 75 mLbio-Si cair dapat direkomendasikan untuk meningkatkan ketahanan bibit kelapa sawit terhadap cekaman kekeringan.  [Kata kunci: bio-Si, klorofil, aktivitas nitrat reduktase, mikroorganisme pelarut silika].
Application of bio-silicic acid to improve yield and fertilizer efficiency of paddy on tidal swamp land Donny Nugroho KALBUADI; Laksmita Prima SANTI; Didiek Hadjar GOENADI; Junita BARUS
Menara Perkebunan Vol. 88 No. 2 (2020): 88 (2), 2020
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i2.378

Abstract

The soils in tidal swamp land as new development area for rice in Indonesia have a considerably low available silica (Si) because very acid condition and no Si fertilization. Therefore, increasing the productivity of rice as Si accumulator plant, in tidal swamp land requires silica fertilizer. This research presented the effect of applications of silicon in the form of orthosilicic acid (H4SiO4) enriched with selected Sisolubilizing fungi, formulated as 4-gram tableted Si fertilizer (BioSilAc) on tidal swamp land soil to improve yield and reduce chemical fertilizer dosage for rice. Field experiment was conducted in Ketapang subdistrict, South Lampung by using a Randomized Block Design (RBD) with six treatments and four replicates. The treatments consisted of (ha-1 season-1): 100% NPK recommended dosage (P1); 100% NPK + 80 BioSilAc tablets (P2); 75% NPK + 80 BioSilAc tablets (P3); 75% NPK + 100 BioSilAc tablets (P4); 50% NPK + 120 BioSilAc tablets (P5); and control (P6). The results showed that P2 treatment increased the highest rice yield in dried harvested grain by 7.6% or equivalent to 500 kg ha-1 compared to P1 treatment. The highest fertilization efficiency can be achieved in P4 treatment which means the NPK recommended dosage can be reduced 25% by adding 100 BioSilAc tablets ha-1 season-1 indicated by insignificantly different productivity compared to that of P1. The Revenue/Cost (R/C) values showed that P2 treatment was economically feasible fertilization practices.