The purpose of this study was to determine the effect of different cooking methods on protein content, fat, cholesterol and taste of Bali beef cattle. The research method used was Completely Randomized Design (RAL) with 4 treatments and 3 replications as follows: P1: Boiled Meat, P2: Steamed Meat, P3: Roasted Meat, P4: Fried Meat. The variables measured were protein content, fat content, cholesterol level, and taste. Data on protein, fat content, and cholesterol levels were analyzed using ANOVA followed by Duncan test. Taste data was analyzed by nonparametric Kruskal-Wallis Test. The result of this study showed that the treatment had a significant effect (P <0.01) on protein content, fat content, and taste of bali beef cattle but not significant (P> 0,05) on beef cholesterol level. It can be concluded that frying and roasting methods produced higher protein and fat content, with taste that tend to be very tasty. However, boiling and steaming methods resulted in lower protein and fat content, with a tendency to taste less tasty. Cholesterol content in each cooking process is relatively similar. ABSTRAK Tujuan dari penelitian ini adalah untuk mengetahui pengaruh cara memasak yang berbeda terhadap kadar protein, lemak, kolesterol dan rasa daging sapi Bali. Metode penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Perlakuan yang dikenakan adalah: P1: Daging Direbus, P2: Daging dikukus, P3: Daging dipanggang, P4: Daging digoreng. Variabel yang diukur adalah kadar protein, kadar lemak, kadar kolesterol, rasa. Data kadar protein, kadar lemak, dan kadar kolesterol dianalisis menggunakan ANOVA (SPSS) dilanjut dengan uji Duncan. Data rasa dianalisis menggunakan nonparametrik Kruskal-Wallis Test. Hasil penelitian ini menunjukkan bahwa perlakuan berpengaruh sangat nyata (P<0,01) terhadap kadar protein, kadar lemak dan rasa daging sapi Bali tetapi berpengaruh tidak nyata (P>0,05) terhadap kadar kolesterol daging sapi Bali. Metode penggorengan dan pemanggangan menghasilkan kandungan gizi protein dan lemak relatif tinggi dengan rasa yang cenderung sangat enak. Metode perebusan dan pengukusan menghasilkan kandungan protein dan lemak lebih rendah dengan rasa yang cenderung kurang enak. Kandungan kolesterol pada setiap proses pemasakan relatif sama.