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All Journal Makara Journal of Technology JURNAL REKAYASA KIMIA & LINGKUNGAN
K. B. Ramachandran
Department of Chemical Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia
Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Electrical & Electronics Engineering Energy Engineering Materials Science & Nanotechnology Mechanical Engineering

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Production of Medium Chain Length Polyhydroxyalkanoates From Oleic Acid Using Pseudomonas putida PGA1 by Fed Batch Culture Marsudi, Sidik; Tan, Irene K.P.; Gan, Seng Neon; Ramachandran, K. B.
Makara Journal of Technology Vol. 11, No. 1
Publisher : UI Scholars Hub

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Bacterial polyhydroxyalkanoates (PHAs) are a class of polymers currently receiving much attention because of their potential as renewable and biodegradable plastics. A wide variety of bacteria has been reported to produce PHAs including Pseudomonas strains. These strains are known as versatile medium chain length PHAs (PHAs-mcl) producers using fatty acids as carbon source. Oleic acid was used to produce PHAs-mcl using Pseudomonas putida PGA 1 by continuous feeding of both nitrogen and carbon source, in a fed batch culture. During cell growth, PHAs also accumulated, indicating that PHA production in this organism is growth associated. Residual cell increased until the nitrogen source was depleted. At the end of fermentation, final cell concentration, PHA content, and productivity were 30.2 g/L, 44.8 % of cell dry weight, and 0.188 g/l/h, respectively.
Effect of Inoculum Age, Carbon and Nitrogen Sources on the Production of Lipase by Candida Cylindracea 2031 in Batch Fermentation Noor, I. M.; Hasan, M.; Ramachandran, K. B.
Jurnal Rekayasa Kimia & Lingkungan Vol 5, No 1 (2006): Jurnal Rekayasa Kimia & Lingkungan
Publisher : Chemical Engineering Department, Syiah Kuala University, Banda Aceh, Indonesia

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Production of extracellular lipase by Candida cylindracea DSMZ 2031 was studied in a seven liters batch bioreactor, using palm oil (PO), palmitic acid (PA), lauric acid (LA), olive oil (OO) and cooking oil (CO) as carbon source. The effect of carbon and nitrogen sources were studied by measuring the lipase activity. The maximum lipase activity was found to be 12.7 kLU on palm oil as carbon source, urea as nitrogen sources and at 36 h inoculum age. This was achieved at a temperature of 30o C, pH of 6.0, agitation speed of 500 rpm and aeration of 1vvm.
Co-Authors Gan, Seng Neon I. M. Noor Irene K. P. Tan, Irene K. P. M. Hasan Seng-Neon Gan, Seng-Neon Sidik Marsudi Tan, Irene K.P.