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Wardiyanto -
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Insidensi Infectious Hypodermal And Hematopoietic Necrosis Virus (IHHNV) Dan Infectious Myonecrosis Virus (IMNV) Pada Udang Putih (Litopenaeus Vannamei) Di Pertambakan Sekitar Teluk Lampung Tahun 2010 Dengan Metode Analisis Polymerase Chain Reaction (PCR) Endang Dwi Kusumaningrum; Wardiyanto -; Toha Tusihadi
e-Jurnal Rekayasa dan Teknologi Budidaya Perairan Vol 1, No 1 (2012)
Publisher : University of Lampung

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Abstract

The purpose of this experiment was to determine the IMNV incidence rate in white shrimp at ponds in Lampung Bay in 2010 and known the pattern of incidence rate from 2007 to 2010. The experiment was conducted on October through December 2010 in ​​culture ponds in the Bay of Lampung and sample examination carried out in Main Center of Mariculture Development of Lampung. The research method was conducted includes, examination of samples by polymerase chain reaction technique and secondary data collection through interviews and data collection, followed by descriptive analysis of data. Prevalence assumption used in this study was 10%. The results showed that there was 7 locations were positive IMNV from 12 locations examination in white shrimp ponds in 2010. The value of incidence was 7.14%, 100%, and 58.33% in 2008, 2009, and 2010, respectively.
Efektifitas Ekstrak Daun Rhizophora Mucronata Dalam Menghambat Pertumbuhan Aeromonas Salmonicida Dan Vibrio harveyi Anisa Suciati; Wardiyanto -; Sumino -
e-Jurnal Rekayasa dan Teknologi Budidaya Perairan Vol 1, No 1 (2012)
Publisher : University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.032 KB)

Abstract

Infectious diseases lead to reducing aquaculture productivity. The using of chemical antibiotic was avoided since their negative effect was known, i.e. created resistant bacteria, contaminating environment and fish meat. The purpose of the research is to find the active compound from Rhizophora mucronata leaf and their activity to inhibit pathogenic bacteria Aeromonas salmonicida and Vibrio harveyi. Extraction procedure conducted by using maceration technique to extract type of mangrove leaf (tip, main leaf, dried leaf), and organic solvent (hexan, ethylacetate, methanol). The result of this research showed that active compound from Rhizopra mucronata give negative response inhibiting Aeromonas salmonicida while postif to Vibrio harveyi . The tip of Rhizophora muncronata leaf is more effective to inhibit pathogenic bacteria, and ethylacetate is the best solvent to extract Rhizophora muncronata leaf. Lethal concentration (LC-50) from Rhizopra macronata is 200 ppm.
Pengaruh Waktu Pemberian Probiotik Yang Berbeda Terhadap Respon Imun NonSpesifik Ikan Mas (Cyprinus Carpio L.) Yang Diuji Tantang Dengan Bakteri Aeromonas Salmonicida Septiarini -; Esti Harpeni; Wardiyanto -
e-Jurnal Rekayasa dan Teknologi Budidaya Perairan Vol 1, No 1 (2012)
Publisher : University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.752 KB)

Abstract

Common carp (Cyprinus carpio) is one of the consumption fish which has high economic value. However, the efforts to increase carp production obstracted by diseases such as furunculosis by A. salmonicida. So that we have to consider the disease prevention method which safer such as probiotic. The aims of this research were to know effect of the time administrations of probiotic on non-specific immune responses and to know the best time administration of probiotic on non-specific immune responses of common carp injected by A. salmonicida. The research was conducted from August to October 2011. The research used three treatments (without administration of probiotic, administration of probiotic once every day, and administration of probiotic once every five days) with four replications. Data of total leukocyte and percentage of differential leukocyte were analyzed by using ANOVA, while RPS and water qualities were analyzed descriptively. The results showed the time administrations of probiotic showed a real impact on improving non-specific immune responses characterized by increased total leukocyte in carp, administration of probiotic once every five days resulted better non-specific immune responses, which had been seen from the highest total leukocyte and the highest RPS after being injected by A. salmonicida.
Co-Authors Anisa Suciati Endang Dwi Kusumaningrum Esti Harpeni Septiarini - Sumino - Toha Tusihadi