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Karlina Samadi
Fakultas Kedokteran Gigi Universitas Airlangga, Surabaya
Dentistry

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Efek Antibiofilm Glass Ionomer Cements dan Resin Modified Glass Ionomer Cements Terhadap Lactobacillus acidophilus Elsandra Novita Halim; Karlina Samadi; Sri Kunarti
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (28.278 KB) | DOI: 10.20473/cdj.v7i2.2017.120-129

Abstract

Background: Risk factors for developing secondary caries are similar to those resulting in primary caries. The marginal seal of a restoration is one of the important factors predicting clinical success. The antibiofilm effect of materials used for the luting cement of oral function affects oral health. Antibiofilm properties of dental luting materials such as Glass Ionomer Cement (GIC) and Resin Modified Glass Ionomer Cement (RMGIC) may improve the restorative treatment outcome. Purpose: This experiment evaluates the antibiofilm effect of GIC and RMGIC on Lactobacillus acidophilus in vitro. Method: Lactobacillus acidophilus served as test microorganism. The quantitative microtiter plate biofilm assays were used to evaluate the antibiofilm effect of the dental luting materials on early-stage biofilm using a direct contact test (DCT) then continued by reading of Optical Density (OD) of biofilm using ELISA reader at a wavelength of 570nm. Result: GIC and RMGIC showed a decrease of OD value from negative control in all groups. The materials’ elute had effect on both bacterial growth with GIC higher then RMGIC to inhibit Lactobacillus acidophilus biofilm formation. Conclusion: The antibiofilm effect of GIC more effective than RMGIC to inhibit Lactobacillus acidophilus biofilm formation. 
Perbedaan ketebalan odontoblast-like cells setelah aplikasi CAPE dan Kalsium Hidroksida Putri Galuh Prawitasari; Karlina Samadi; Ari Subiyanto
Conservative Dentistry Journal Vol. 8 No. 2 (2018): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.899 KB) | DOI: 10.20473/cdj.v8i2.2018.118-122

Abstract

Background : CAPE is the main component of propolis, it has several biology and fpharmocological advantages as antioxidant, antiinflammation, anticancer and as an immunomodulator. There were the research before of CAPE is capable to stimulate the production of the TGF-β 1 and collagen sintesis by the pulp tissue with many superiority compare to Ca(OH)2, recently Ca(OH)2 is  the gold standard for pulp capping treatment procedure. Objective : To determine and compare the effectiveness of CAPE and Ca(OH)2 to the thickness of odontoblast-like cells in rat pulp tissue. Methods : Maxillary first molar tooth of wistar mice was class 1 preparated until the pulp opened, then Ca(OH)2 was aplicated for 14 and 28 days, CAPE for 14 and 28 days. After application of Ca(OH)2 and propolis extract, the tooth was filled with RMGIC. Teeth were extracted on defined day and processed for histological evaluation. Result : There is a significant difference in the thickness of odontoblast-like cells after application of CAPE for 14 days with Ca(OH)2 for 14 days and CAPE for 28 days with Ca(OH)2 for 28 days. Conclusion : odontoblast-like cells after aplication of CAPE extract material is thicker than Ca(OH)2.
Co-Authors Ari Subiyanto Elsandra Novita Halim Putri Galuh Prawitasari Sri Kunarti