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All Journal Jurnal Respirologi Indonesia Jurnal Kajian Ilmu Pendidikan (JKIP)
Eko Prasetyo
Universitas Brawijaya
Education Health Professions Medicine & Pharmacology Social Sciences Other

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Immunogenicity Test of ESAT-6/CFP-10 Mycobacterium tuberculosis (Indonesian Strain) Recombinant Protein Fusion: TNF-α, IL-17 and CD4+ T Cells Expression in PBMC Culture Eko Prasetyo; Triwahju Astuti; Nunuk Sri Muktiati; Maimun Z Arthamin
Jurnal Respirologi Indonesia Vol 39, No 3 (2019)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1250.041 KB) | DOI: 10.36497/jri.v39i3.64

Abstract

Background: Bascillus Calmette Guѐrin vaccination has not provided protection against TB in adults. ESAT-6/CFP-10 Mtb recombinant protein fusion as a vaccine candidate can stimulate the body's immune response. Interleukin-17, TNF-α and CD4+ play a major role against TB. This study aims to determine that the recombinant protein fusion ESAT-6/CFP-10 Mtb can stimulate TNF-α, IL-17 and CD4+ T cells expression in PBMC culture. Methods: Design of this study is experimental study. Number of research sample per group of 8 subjects. The subjects: healthy, TB contact and TB patients were taken their peripheral blood sample and treated with ESAT-6/CFP-10 Mtb recombinant protein fusion. TNF-α CD4+ T cells were measured by ELISA. Flow cytometry is used to measure IL-17 and CD4+ Tcells. As standard protocol research on tuberculosis vaccine, each subject also treated without antigen and with PPD. Results: There was no significant increase in the administration of ESAT-6/CFP-10 Mtb fusion compared without antigen on TNF-α expression of CD4+ (P=0.202), expression of IL-17 CD4+ (P=0.994) and percentage of CD4+ T cells (P=0.183). ESAT-6/CFP-10 Mtb Fusion was able to stimulate expression of TNF-α CD4+ in healthy subjects (29.91±1.23pg/ml), TB contact (32.21±4.02pg/ml) and TB patients (33.35±8.41 pg/ml). Expression IL-17 CD4+ in healthy subjects (33.24 ± 39.01%), TB contact (23.88 ± 21%) and TB patients (51.93 ± 36%). CD4+ T cell expression in healthy subjects 30.64 ± 7.63%, TB contact 24.58 ± 5.24% and TB patients (40.73±2.63%). Conclusions: ESAT-6/CFP-10 Mtb recombinant proteins fusion may stimulate the production of TNF-α, IL-17 CD4+ and CD4+ T cells in all subject. Expression of TNF-α, IL-17 CD4+ and CD4+ T cells in the healthy group, indicated that the ESAT-6/CFP-10 recombinant protein fusion has the potential as vaccine candidate. (J Respir Indo. 2019; 39(3):160-8)
Evaluasi Efektivitas Aktivasi DOI Terhadap Kesiapan Tata Kelola Jurnal Studi Pre–Post Pada Jurnal Perguruan Tinggi Vokasi Fitriana Rakhma Dhanias; Eko Prasetyo
Jurnal Kajian Ilmu Pendidikan (JKIP) Vol. 7 No. 1 (2026): Jurnal Kajian Ilmu Pendidikan (JKIP)
Publisher : Lembaga Riset dan Inovasi Almatani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55583/jkip.v7i1.1992

Abstract

Aktivasi Digital Object Identifier (DOI) merupakan salah satu indikator penting dalam peningkatan kualitas tata kelola jurnal ilmiah, khususnya dalam mendukung visibilitas, keterlacakan sitasi, dan standarisasi publikasi. Penelitian ini bertujuan untuk mengevaluasi efektivitas aktivasi DOI terhadap kesiapan tata kelola jurnal di lingkungan perguruan tinggi vokasi dengan menggunakan desain studi pre–post. Data dikumpulkan melalui observasi, dokumentasi, dan kuesioner kepada pengelola jurnal sebelum dan sesudah implementasi DOI. Aspek kesiapan yang dianalisis meliputi kelengkapan metadata, konsistensi pengindeksan, manajemen artikel, kepatuhan terhadap standar OJS, serta pemahaman pengelola terhadap etika dan manajemen publikasi ilmiah. Hasil penelitian menunjukkan adanya peningkatan signifikan pada kualitas pengelolaan jurnal setelah aktivasi DOI, terutama pada aspek keteraturan metadata, kemudahan penelusuran artikel, serta peningkatan kesadaran pengelola terhadap standar internasional pengelolaan jurnal. Temuan ini mengindikasikan bahwa aktivasi DOI tidak hanya berfungsi sebagai identitas digital artikel, tetapi juga berperan strategis dalam mendorong kesiapan dan profesionalisme tata kelola jurnal perguruan tinggi vokasi menuju akreditasi dan indeksasi bereputasi.
Co-Authors Fitriana Rakhma Dhanias Maimun Z Arthamin Nunuk Sri Muktiati Triwahju Astuti