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All Journal Molekul: Jurnal Ilmiah Kimia

Purwati Purwati

Program Studi Kimia, Jurusan MIPA, Fakultas Sains dan Teknik UNSOED

Author-ID : 3605735

Chemical Engineering, Chemistry & Bioengineering Chemistry

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SKRINING SENYAWA METABOLIT SEKUNDER DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETILASETAT DAUN WEDUSAN (Eupatorium odoratum) Purwati Purwati; Undri Rastuti
Molekul Vol 4, No 2 (2009)
Publisher : Universitas Jenderal Soedirman

Oxidation process is a natural process which always occurs in fat. The process affects and decreases the fat quality. Oxidation in fat can be hampered by the addition of antioxidant. Antioxidant activity of wedusan leaf has to be studied to know the possibility of wedusan leaf as an antioxidant. Hence, the aims of the research were to determine the antioxidant activity of ethyl acetate extract of wedusan leaf using TBA method, and to compare the antioxidant activity of wedusan leaf and that of BHT. The research method consisted of sample preparation, extraction, and determination of antioxidant activity using TBA method. Wedusan leaf was extracted by maceration using n-hexane and ethyl acetate solvents. The n-hexane extract was 2.90 gram, whereas ethyl acetate extract was 13.12 gram. Based on qualitative screening on secondary metabolites, ethyl acetate extract contained flavonoid. The results from GC-MS indicated that ethyl acetate extract contained methyl heptadecanoic, methyl-13-octadecenoic, 14,16-octadecadienal, and octadecanoic acid. The order of inhibition activity of antioxidant were 0.05% (w/v) of BHT > 0.15% (w/v) of ethyl acetate extract > 0.10% (w/v) of ethyl acetate extract > 0.05% (w/v) of ethyl acetate extract.

PEMANFAATAN MEMBRAN NATA DE COCO SEBAGAI MEDIA FILTRASI UNTUK REKOVERIMINYAK JELANTAH Senny Widyaningsih; Purwati Purwati
Molekul Vol 8, No 1 (2013)
Publisher : Universitas Jenderal Soedirman

Peningkatan kualitas minyak jelantah telah dilakukan dengan metode filtrasi menggunakan membran selulosa bakterial yang terbuat dari nata de coco. Pembuatan membran nata de coco dilakukan dengan cara memurnikannata de coco hasil proses fermentasi bakteri Acetobacter xylinum. Karakterisasi membran meliputi berat jenis, nilai fluks, dan koefisien rejeksi. Proses filtrasi minyak jelantah menggunakan tekanan sebesar 4 kgf/cm2 dengan metode dead-end. Hasil penelitian menunjukkan bahwa karakterisasi membran nata de coco memiliki berat jenis sebesar 0,6314 g/cm3, nilai fluks air sebesar 104,021 L/m2.jam, fluks minyak sebesar 1,004 L/m2.jam dan fluks Dekstran T-500 sebesar 52,208 L/m2.jam. Nilai rejeksi membran sebesar 15,11%. Peningkatan kualitas minyak jelantah dibanding sebelum rekoveri dapat dilihat dari penurunan angka asam sebesar 54,95%, kadar air sebesar 93,22%, serta peningkatan angka penyabunan sebesar 29,09% dan angka iod sebesar 8,14%.

UJI AKTIVITAS ANTIKANKER EKSTRAK ETANOL DAUN Rhizopora mucronata TERHADAP SEL MYELOMA Hartiwi Diastuti; Warsinah Warsinah; Purwati Purwati
Molekul Vol 3, No 2 (2008)
Publisher : Universitas Jenderal Soedirman

The mangrove plant have been long used the people for traditional medicine to cure various diseases, one of them to cancer therapy. Investigation the anticancer potent of R. mucronata has not been carried yet. This research was aimed to toxicity test of R. mucronta leaves extracts againts Artemia salina Leach, then cytotoxicity test of R. mucronata leave extracts againts Myeloma cancer cells. The extraction of R. mucronata leaves was peformed by maseration with ethanol. The ethanol extracts was partitioned with chloroform, ethylacetate and methanol. The ethanol extracts of R. mucronata leaves respectively was examined their toxicity againts A. salina Leach larv. The toxic exctracts was examined their citotoxicity againts Myeloma cells. The ethanol extract of R. mucronata leaves has toxic character because has LC50 < 1000 mg/mL. Citotoxicity test againts Myeloma cells showed that the chloroform fraction of ethanol extract of R. mucronata leaves have a high activity against Myeloma cell, LC50 value equal to 28,72 mg/mL. Phytochemical study showed that the active fractions contained terpenoid and alkaloid.

UJI AKTIVITAS ANTIOKSIDAN HASIL DEGRADASI LIGNIN DARI SERBUK GERGAJI KAYU KALBA (Albizia falcataria) DENGAN METODE TBA (Thio Barbituric Acid) Undri Rastuti; Purwati Purwati
Molekul Vol 5, No 2 (2010)
Publisher : Universitas Jenderal Soedirman

Antioxidants are compounds that can delay, retard or inhibit the oxidation reaction. Lignin is a natural polymer consisting of monomeric substituted phenols. Wood lignin degradation Kalba (Albizia falcataria) yields substituted phenol. The purpose of this study was to test the antioxidant activity of compounds of lignin degradation products Kalba using TBA (Thiobarbituric Acid). Wood lignin degradation products Kalba tested antioxidant activity using the TBA method. Phase test phase of this antioxidant activity is sample preparation, determination of the maximum wavelength, determination of equilibrium time, absorbance measurements and determination of the percentage of inhibition. The wavelength maximum for BHT test solution was obtained at 530 nm. The stability of absorbance achieved after 80 minutes equilibrium time. BHT test solution and sample solution containing the degradation of lignin 0.10% (w/v) increased but not as sharp as the absorbance of control, this suggests that the degradation of wood lignin Kalba have activity as an antioxidant, which relative minimize 13,70 % compare with BHT.

Co-Authors Hartiwi Diastuti Senny Widyaningsih Undri Rastuti Warsinah Warsinah

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