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All Journal Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Natural B
Muhaimin Rifa’i
Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya, Malang
Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience

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Effects of Gamma Radiation Exposure to Hematopoietic Cells on Bone Marrow Qomariyah, Nurul; Rifa’i, Muhaimin; Juswono, Unggul P.
Natural B, Journal of Health and Environmental Sciences Vol 2, No 1 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.472 KB) | DOI: 10.21776/ub.natural-b.2013.002.01.4

Abstract

Radiotherapy is a treatment for cancer which use external radiation sources with the aim to damage the DNA in the nucleus of the cancer chromosomes and cause necrosis of cancer cells. The exposure of radiation to the spinal cord as the site of blood cell formation would change the hematopoietic circulation system. The effect of gamma radiation exposure on decreasing of hematopoietic cells quantity is still unknown and it needs further experiment to determine the effect the radiation and the effect of P. niruri  extract giving on the reduction of immune cells quantity which are CD34+ and B220+ cells in the bone marrow. In this experiment, five variations of the dose radiation was used. The dose were 100 rad, 200 rad, 300 rad, 400 rad and 500 rad and use single dose of P. niruri as much 250 mg/kg BB. The dose of 100 rad radiation was given daily and P. niruri extract was given for 14 days before and during radiation. The amount of the CD34+ and B220+ cells is counted and analyzed by Flow cytometry. The result of this experiment showed that the giving of gamma ray irradiation reduced the amount of immune cells in the bone marrow. The increasing radiation dose was caused increasing of the reduction of cells immune quantity. The giving of P.niruri extract inhibits hematopoietic cell through increasing of the absolut amount of CD34+ cell in the bone marrow.
KEMAMPUAN SEL LIMPA NORMAL MENCEGAH TERJADINYA PENYAKIT AUTOIMMUNE PADA MENCIT IL-2Rβ ββ β -/- Rifa’i, Muhaimin
Jurnal Kedokteran Brawijaya Vol 21, No 1 (2005)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.37 KB) | DOI: 10.21776/ub.jkb.2005.021.01.2

Abstract

Adoptive transfer is a potentially curative treatment for both inherited and acquired diseases of hematopoietic compartment and organ failure. The aim of this experiment was to know the potency of normal unfractionated spleen cells to prevent pathological autoimmunity in IL-2Rβ-/-mice. Here, we show that the normal unfractionatedspleen cells have a capacity to prevent autoimmune diseases when adoptively transferred to IL-2Rβ-/- mice. Intravenously, and adoptively transferred of normal unfractionated spleen cells (107) to IL-2Rβ-/-mice (3 wks) gave an evidence on preventing the lethal phenotype due to autoimmune disease in IL-2Rβ-/-mice. The donor cells have a capacity to reverse the status of recipient cells from memory to naïve type. In addition, donorcells also have a capacity to regulate the development of granulocyte cells into normal level of homeostasis. These findings suggest that normal unfractionated spleen cells  could in the future be used in clinical settings to cure autoimmune diseases. Keywords :autoimmune, spleen cells, IL-2Rβ-/-, mice
The Effect of Moringa oleifera Leaves and VipAlbumin® on The Immune System of Diabetes Mellitus Balb/C Mice Model Almabrouk, Suheer Khalleefah; Dwi, Noviana; Rahayu, Sri; Rifa’i, Muhaimin
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1126.629 KB) | DOI: 10.21776/ub.jels.2019.009.03.05

Abstract

Diabetes Mellitus is known as a disease grown worldwide rapidly prevalent day by day. This disease caused by a chronic hyperglycemic condition and also glucose intolerance resulting from defects in insulin secretion, insulin action, or both. Moringa oleifera is one of the plants used in most developing countries for traditional medicine for treating diabetes. In this research, Moringa oleifera mixed with VipAlbumin®, taken from snakehead fish albumin. Albumin found as an antioxidant against ROS. This research aimed to evaluate the effect of Moringa oleifera mixed with VipAlbumin® supplement towards the profile changing of T-cell, (CD4+ CD8+) B220+, CD4+ IFN-γ, and TNF-α. The experiments were done by dividing Balb/c mice into five groups and induced with 100 mg.BW-1 STZ. Afterward, the mix of Moringa oleifera and VipAlbumin® orally administrated into five different doses. Negative control contains of healthy mice, Positive dose administrated with 145 mg.kg bw-1 STZ, Dose 1 of 100 mg.kg bw-1 (Mo) + 416.25 mg.kg bw-1 (A), Dose 2 of 150 mg.kg bw-1 (Mo) + 208.15 mg.kg bw-1 (A), Dose 3 of 50 mg.kg bw-1 (Mo) + 624.375 mg.kg bw-1 (A). One way ANOVA was applied to analyze the data with p-value 0.05% and combined with Tukey test using SPSS version 16 for Windows. The results showed that a relative number of CD4 and CD8 T cells decreased in dose 3 of Moringa oleifera and Albumin, as well as B220 in dose 3 gave a significant decreased compared to healthy mice (p<0.05). The inflammation showed decreasing after treatment with dose 3 of Moringa oleifera and albumin extract. Taken together that proinflammatory cytokines decreased after treatment compared to a positive group. Keywords: Albumin, Inflammation, Moringa oleifera, T cells.
Co-Authors Almabrouk, Suheer Khalleefah Dwi, Noviana P Purwanto SRI RAHAYU Unggul P. Juswono