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All Journal HAYATI Journal of Biosciences JURNAL TEKNOLOGI LINGKUNGAN BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Hidrosfir Indonesia Teknologi Indonesia
Rini Riffiani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Environmental Science

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Journal : BERITA BIOLOGI

 1 
POTENSI Rhodococcus pyridinovorans GLB5 SEBAGAI BIOKATALIS DALAM KONVERSI SENYAWA METHIL SIANIDA DAN PHENIL SIANIDA [Potential of Rhodococcus pyridinovrans GLB5 as Biocatalistin Methyl and Phenyl Cyanide Conversion] Sulistinah, Nunik; Riffiani, Rini; Sunarko, Bambang
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3384.218 KB) | DOI: 10.14203/beritabiologi.v15i1.2856

Abstract

Nitrile and amide bioconversion have received increasing attention due to their ability to provide a range of commercially important chemicals. The experiment was conducted to investigate the potential of bacterial isolate GLB5 to convert methyl cyanide and phenyl cyanide. The samples were collected from various industrial waste. Selection of isolates to utilize  these substrates as a sole source of energy, carbon and nitrogen was conducted on 96 whell microtitter plates, based on the growth ability using INT (Iodo nitrotetrazolium chloride) reagent. Based on the growth  pattern, it showed that the bacterial isolate GLB5 grew well and it was capable of utilizing  methyl and phenyl cyanide compound as the sole source of carbon and nitrogen.  The isolate GLB5 was isolated from industrial waste of Batik factory in Cirebon, and  identified as Rhodococcus pyridinovorans. Bioconversion of methyl cyanide using whole cells of R. pyridinovorans GLB5 showed that ethanamide (C2H5NO) and ethanoic acid (C2H4O2) were detected. Formation of ethanamide and ethanoic acid as the product of bioconversion, indicated that the nitrile hydratase and amidase enzymes  involved in the bioconversion process. Phenyl carboxamide (C7H7NO) as the product of phenyl cyanide bioconversion was also detected,  although  in  low  concentration. In this study, R. pyridinovorans GLB5 was capable of completely converting 300 mM methyl cyanide to ±  140 mM ethanoic acid in relatively short times (<60 minutes).
KERAGAMAN BAKTERI PENGHASIL ENZIM PENGHIDROLISIS NITRIL DI PULAU ENGGANO BENGKULU [Diversity of Nitrilase Producing Bacteria in Enggano Island, Bengkulu] Riffiani, Rini; Sulistinah, Nunik
BERITA BIOLOGI Vol 16, No 3 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3647.412 KB) | DOI: 10.14203/beritabiologi.v16i3.2243

Abstract

Potential nitrile degrading microbes have been isolated from marine sponge, marine water and soil in Enggano Island. Nitrilase enzyme has a function in degrading nitrile compund. Nitrilases are important industrial enzymes because of their ability to produce biologically active to degrade enantiomers, such as S-(+)-1-(4’-isobutylphenyl) propionic acid (S-(+)-ibuprofen) and R-(-) mandelic acid. Mandelic acids, which are important as pharmaceutical intermediates, can be produced in enantiomerically pure form by the hydrolysis of their corresponding nitrile. The aim of the study was to explore the diversity of nitrile degrading bacteria in Enggano Island, and their ability to utilize nitrile as a substrate growth. Screening of such microbes were carried out by using microtitter plate method based on growth ability tested by INT (Iodonitrotetrazoliumchloride). Degradation product was determined by High Performance Liquid Chromatography (HPLC). Seventy nine bacteria were able to grow on acetamide, acetonitrile, benzonitrile, adiponitrile, mandelonitrile, succinonitrile, lactonitrile, dan benzilcyanide as the sole source of carbon and nitrogen. Two isolates, YIM 56238 and PO69, have shown to enantioselectively hydrolyze racemic mandelonitrile to mandelic acid. Based on 16S rRNA gene identification, these bacteria have the highest sequence similarity to Microccous endophyticus strain YIM 56238 and Rhodococcus pyridinivorans strain PO69.
Co-Authors BAMBANG SUNARKO Ivan Permana Putra NUNIK SULISTINAH Nurhayat, Oktan Dwi Taridala, Sitti Aida Adha