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All Journal HAYATI Journal of Biosciences JURNAL TEKNOLOGI LINGKUNGAN BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Hidrosfir Indonesia Teknologi Indonesia
Rini Riffiani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Environmental Science

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Journal : JURNAL BIOLOGI INDONESIA

 1 
Penguncilan Gen Penyandi Enzim Nitrilase Enam Isolat Bakteri Unggulan Riffiani, Rini; Sulistinah, Nunik; Sunarko, Bambang
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3206.948 KB) | DOI: 10.14203/jbi.v11i1.2155

Abstract

Indonesia sebagai negara tropis memiliki biodiversitas yang sangat tinggi. Keanekaragaman hayati ini diperkirakan mencerminkan keanekaragaman kimiawi sekaligus keragaman genetik yang dapat dimanfaatkan untuk mencari biokatalis baru. Enam isolat bakteri yaitu GLB5, LP3, TPIK, MICC, 23A2, dan 23A2 telah diisolasi dari berbagai limbah industri dan mempunyai potensi sebagai pendegradasi nitril.  Pengucilan, identifikasi dan purifikasi gen penyandi enzim nitrilase dari keenam isolat bakteri tersebut  telah dilakukan. Dari kegiatan penelitian ini 3 isolat bakteri unggulan, yaitu GLB5, LP3, dan TPIK teridentifikasi sebagai Rhodococcus pyridinivorans, sedangkan  MICC teridentifikasi sebagai Bacillus substilis, 23A2 teridentifikasi sebagai Brevibacillus brevis, dan 26A2 teridentifikasi sebagai Microbacterium oxydans. Peta untaian basa nukleutida dari gen penyandi enzim nitrilase dari ketiga isolat yaitu GLB5, LP3, dan TPIK telah terpetakan dengan ukuran gen nitrilase sebesar 960 bp. Hasil analisis dengan BLASTN memperlihatkan bahwa fragmen gen nitrilase yang diamplifikasi dengan primer Nit1101F dan Nit1101R mempunyai homologi yang tinggi terhadap Rhodococcus rhodochrous strain tg1-A6 nitrilase gen dengan persentase kesamaan sebesar 96% . Kata Kunci: Gen, isolasi, nitril, degradasi, enzim 
Pengembangan Sistem Deteksi Senyawa Sianogen dalam Ubi Kayu (Manihot esculenta Crantz) dengan Pendekatan Enzimatis Sulistinah, Nunik; Riffiani, Rini; Sunarko, Bambang
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i1.332

Abstract

Picrate paper test kit for the semi–quantitative determination of cyanogenic potential was developed in thisexperiment. The method is relatively simple, easy to use and might be applicable in the field by unskilled person.Paper test was attached on tubes containing sample (100 mg) in aquadest (0,5 mL) and then was immediatelycovered tightly and incubated overnight at room temperature. The colour of picrate paper test changed graduallytowards reddish brown, and its colour was compared with standart colour chart which included 0-800 ppm cyanidethat was also developed in this study. The reddish brown colour of paper test was correlated with cyanideconcentration on the sample. In order to obtain a more accurate detection of cyanogenic compound the paper testwas eluted with 5 mL water or aquadest and the absorbance was measured at 510 nm.Keywords: cyanogenic potential, picrate paper test, semi-quantitative method, simple method, cassava (Manihotesculenta Cranz)
Isolasi Bakteri Pendegradasi Phenanthrene dari Batanta-Salawati Raja Ampat Papua Riffiani, Rini
JURNAL BIOLOGI INDONESIA Vol 6, No 2 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (652.216 KB) | DOI: 10.14203/jbi.v6i2.3153

Abstract

ABSTRACTIsolation of Bacteria Degrading Phenanthrene in Batanta- Salawati Districts Raja AmpatPapua. Polycyclic aromatic hydrocarbons (PAHs) are important environmental contaminantsin soil and water. These compounds have a potential risk to human health, as many of them arecarsinogenic and toxic to marine organisms such as diatome, gasthrophode, mussel, and fish.Phenanthrene is one of the hazardous hydrocarbon compounds. The purpose of this researchwas to characterize microbial strains from Batanta-Salawati Raja Ampat Papua Island and theirability to remove phenanthrene. Two isolates were identified at their physiological characteristicsbased on salinity tolerance, pH tolerance and the composition of nitrogen base. Molecularidentification based on 16S rRNA gene sequences indicated that bacteria had the highestsimilarity with Rhodobacteraceae bacterium F9 and Roseobacter sp. RW 37.Rhodobacteraceae bacterium F9 could grow optimum on ONR7a media with 5% salinity andat pH of 5-7,5 while Roseobacter sp. RW 37 could grow optimum on ONR7a media with 2%salinity and at pH of 6,2-7,5.Key words: Phenanthrene, physiological characteristic, molecular identification, Raja Ampat
Penapisan Mikroba Laut Perombak Senyawa Nitril dan Protein yang Diisolasi Dari Spons di Perairan Ternate Riffiani, Rini; Sulistinah, Nunik
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.562 KB) | DOI: 10.14203/jbi.v6i3.3143

Abstract

ABSTRACTScreening of Nitrile and Protein-Degrading Marine Bacteria Isolated from Sponge in TernateSea Water. Thirty three marine bacteria have been isolated from marine sponge in Ternate byenrichment culture. Screening bacteria-degrading nitrile was done by microtitter plate methodbased on growth ability tested by Iodonitrotetazolium chloride. Product of nitrile degradationwas determined by Gas Chromatography (GC) and the potential bacteria-degrading proteinwas also screened by using selected media which contained casein. The results showed thattwenty one isolates were able to show the clearing zone in selected media. Five isolatescapable of utilizing acetamide as the sole source of carbon and nitrogen. Acetate and ammoniaproduced for hydrolysis acetonitrile by using resting cell of Lysobacter sp.Key words: Nitrile, bacterium, sponge, Ternate
ISOLASI BAKTERI PENDEGRADASI PHENANTHRENE DARI BATANTA-SALAWATI RAJA AMPAT PAPUA Riffiani, Rini
JURNAL BIOLOGI INDONESIA Vol 6, No 2 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i2.3153

Abstract

ABSTRACTIsolation of Bacteria Degrading Phenanthrene in Batanta- Salawati Districts Raja AmpatPapua. Polycyclic aromatic hydrocarbons (PAHs) are important environmental contaminantsin soil and water. These compounds have a potential risk to human health, as many of them arecarsinogenic and toxic to marine organisms such as diatome, gasthrophode, mussel, and fish.Phenanthrene is one of the hazardous hydrocarbon compounds. The purpose of this researchwas to characterize microbial strains from Batanta-Salawati Raja Ampat Papua Island and theirability to remove phenanthrene. Two isolates were identified at their physiological characteristicsbased on salinity tolerance, pH tolerance and the composition of nitrogen base. Molecularidentification based on 16S rRNA gene sequences indicated that bacteria had the highestsimilarity with Rhodobacteraceae bacterium F9 and Roseobacter sp. RW 37.Rhodobacteraceae bacterium F9 could grow optimum on ONR7a media with 5% salinity andat pH of 5-7,5 while Roseobacter sp. RW 37 could grow optimum on ONR7a media with 2%salinity and at pH of 6,2-7,5.Key words: Phenanthrene, physiological characteristic, molecular identification, Raja Ampat
PENAPISAN MIKROBA LAUT PEROMBAK SENYAWA NITRIL DAN PROTEIN YANG DIISOLASI DARI SPONS DI PERAIRAN TERNATE Riffiani, Rini; Sulistinah, Nunik
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3143

Abstract

ABSTRACTScreening of Nitrile and Protein-Degrading Marine Bacteria Isolated from Sponge in TernateSea Water. Thirty three marine bacteria have been isolated from marine sponge in Ternate byenrichment culture. Screening bacteria-degrading nitrile was done by microtitter plate methodbased on growth ability tested by Iodonitrotetazolium chloride. Product of nitrile degradationwas determined by Gas Chromatography (GC) and the potential bacteria-degrading proteinwas also screened by using selected media which contained casein. The results showed thattwenty one isolates were able to show the clearing zone in selected media. Five isolatescapable of utilizing acetamide as the sole source of carbon and nitrogen. Acetate and ammoniaproduced for hydrolysis acetonitrile by using resting cell of Lysobacter sp.Key words: Nitrile, bacterium, sponge, Ternate
PENGEMBANGAN SISTEM DETEKSI SENYAWA SIANOGEN DALAM UBI KAYU (MANIHOT ESCULENTA CRANTZ) DENGAN PENDEKATAN ENZIMATIS Sulistinah, Nunik; Riffiani, Rini; Sunarko, Bambang
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i1.332

Abstract

Picrate paper test kit for the semiâ??quantitative determination of cyanogenic potential was developed in thisexperiment. The method is relatively simple, easy to use and might be applicable in the field by unskilled person.Paper test was attached on tubes containing sample (100 mg) in aquadest (0,5 mL) and then was immediatelycovered tightly and incubated overnight at room temperature. The colour of picrate paper test changed graduallytowards reddish brown, and its colour was compared with standart colour chart which included 0-800 ppm cyanidethat was also developed in this study. The reddish brown colour of paper test was correlated with cyanideconcentration on the sample. In order to obtain a more accurate detection of cyanogenic compound the paper testwas eluted with 5 mL water or aquadest and the absorbance was measured at 510 nm.Keywords: cyanogenic potential, picrate paper test, semi-quantitative method, simple method, cassava (Manihotesculenta Cranz)
PENGUNCILAN GEN PENYANDI ENZIM NITRILASE ENAM ISOLAT BAKTERI UNGGULAN Riffiani, Rini; Sulistinah, Nunik; Sunarko, Bambang
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2155

Abstract

Indonesia sebagai negara tropis memiliki biodiversitas yang sangat tinggi. Keanekaragaman hayati ini diperkirakan mencerminkan keanekaragaman kimiawi sekaligus keragaman genetik yang dapat dimanfaatkan untuk mencari biokatalis baru. Enam isolat bakteri yaitu GLB5, LP3, TPIK, MICC, 23A2, dan 23A2 telah diisolasi dari berbagai limbah industri dan mempunyai potensi sebagai pendegradasi nitril.  Pengucilan, identifikasi dan purifikasi gen penyandi enzim nitrilase dari keenam isolat bakteri tersebut  telah dilakukan. Dari kegiatan penelitian ini 3 isolat bakteri unggulan, yaitu GLB5, LP3, dan TPIK teridentifikasi sebagai Rhodococcus pyridinivorans, sedangkan  MICC teridentifikasi sebagai Bacillus substilis, 23A2 teridentifikasi sebagai Brevibacillus brevis, dan 26A2 teridentifikasi sebagai Microbacterium oxydans. Peta untaian basa nukleutida dari gen penyandi enzim nitrilase dari ketiga isolat yaitu GLB5, LP3, dan TPIK telah terpetakan dengan ukuran gen nitrilase sebesar 960 bp. Hasil analisis dengan BLASTN memperlihatkan bahwa fragmen gen nitrilase yang diamplifikasi dengan primer Nit1101F dan Nit1101R mempunyai homologi yang tinggi terhadap Rhodococcus rhodochrous strain tg1-A6 nitrilase gen dengan persentase kesamaan sebesar 96% . Kata Kunci: Gen, isolasi, nitril, degradasi, enzim 
Co-Authors BAMBANG SUNARKO Ivan Permana Putra NUNIK SULISTINAH Nurhayat, Oktan Dwi Taridala, Sitti Aida Adha