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All Journal Journal of Tropical Animal Science and Technology
Agustinus A Dethan
Universitas Timor
Agriculture, Biological Sciences & Forestry

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The Effect of Filteret Sugarcane Juice in Egg Yolk Citrate on Viability and Spermatozoa Abnormality, and pH of Bali Catle Semen Rindiyani Y Tanii; Agustinus A Dethan; Theresia Ika Purwantiningsih
Journal of Tropical Animal Science and Technology Vol 4 No 1 (2022): Journal of Tropical Animal Science and Technology
Publisher : Program Studi Peternakan, Fakultas Pertanian Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v4i1.1098

Abstract

This study aims to determine the effect of the sugarcane juice diluent in egg yolk citrate on viability, spermatozoa abnormality, and pH of Bali bull semen. This study has been carried out in Poultry Housing of Animal Science Study Program, and the Laboratory of the Faculty of Agriculture, University of Timor. Semen is collected from a a bull, ± 4.5 years old, in healthy condition. The method used in this study was a completely randomized design method with 5 treatments and 4 replications. The treatment giving are: T0 (diluent citrate-egg yolk without sugarcane juice); T1 (diluent citrate-egg yolk + 5ml sugarcane juice); T2 (diluent citrate-egg yolk + 10 ml sugarcane juice); T3 (diluent citrate-egg yolk + 15 ml sugarcane juice) and T4 (diluent citrate-yolk + sugar cane juice 20 ml). The variables measured were viability, abnormal spermatozoa, and semen pH of Bali bull. The results showed that T4 treatment with a 20 ml sugarcane juice level showed the best value for spermatozoa viability (92.875%), spermatozoa abnormality (6.375%), and semen pH (6.6). Conclusion: that the level of 20 ml of sugarcane juice in semen diluent can maintain the life of Bali bull spermatozoa.
Quality of Landrace Semen in Yolk Citrate Cement which Plus Glucose with Different Concentrations Albertus Baku; Agustinus A Dethan; Paulus Klau Tahuk
Journal of Tropical Animal Science and Technology Vol 4 No 1 (2022): Journal of Tropical Animal Science and Technology
Publisher : Program Studi Peternakan, Fakultas Pertanian Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v4i1.1268

Abstract

The purpose of this study was to determine the effect of using yolk citrate diluent with different concentrations of glucose added on the motility, viability, abnormalities and pH of landrace pig cement.The cement used was fresh cement from a 3 year old Landrace pig stud.This study used an experimental method using a completely randomized design (CRD) with four treatments (P1 5%, P2 10%, P3 15% and P4 20%) and four replications so that there were 16 experimental units.The results showed that the concentration of glucose in the yolk citrate diluent was not significantly different (P>0.05) on the motility of spermatozoa with the average value of each treatment being P1 (65%), P2 (60%), P3 (63, 8%) and P4 (67.5%).The percentage of spermatozoa viability was very significantly different (P<0.01) between treatments, where in treatment P2 was relatively the same as treatment P3 and P4 but higher than treatment P1 with the value of each treatment P1 (83%), P2 (90.1 %), P3 (93.5%) and P4 (91.5%). Percentage of spermatozoa abnormalities resulting from analysis of variance (ANOVA) from each treatment there was a non-significant difference (P>0.05) where the values ​​were P1 (8.2%), P2 (9.2%), P3 (8.1%) and P4 (9.2%). The pH was significantly different (P<0.05) between treatments, where P1 treatment was relatively the same as P2 but higher than P4 treatment. On the other hand, treatment P3 was relatively the same as treatment P1, P2 and P4, with the values ​​of each treatment being P1 (8.36), P2 (7.99), P3 (7.676) and P4 (6.83). It can be concluded that the use of different glucose concentrations in the egg yolk citrate diluent with good quality, namely in the P3 treatment with a glucose concentration of 15%, the average percentage of individual motility was 64%, the percentage of live spermatozoa was 93.5%, the percentage of abnormal spermatozoa was 8.1%. and the average pH of cement is 7.68. It was concluded that the quality semen of Landrace pig could be improved optimally using glucose concentration in egg yolk citrate diluent by 15%.
Co-Authors Albertus Baku P.K. Tahuk Rindiyani Y Tanii Theresia Ika Purwantiningsih