Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal Current Research on Biosciences and Biotechnology
Ida Susanti
Agency for the Assessment and Application of Technology
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Materials Science & Nanotechnology Medicine & Pharmacology

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
The effect of ascorbic acid and soybean protein hydrolysate to iron binding capacity Sri Peni Wijayanti; Erma Maryana; Ida Susanti; Noer Laily
Current Research on Bioscences and Biotechnology Vol. 2 No. 1 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2020.2.1/QSEB7056

Abstract

The study aimed to investigate the effect of ascorbic acid and soybean protein hydrolysate to iron-binding capacity. BPPT (Agency for the Assessment and Application of Technology) developed soybean hydrolysate, which was obtained by thermal and enzymatic hydrolysis. Ascorbic acid was added in sample preparation with composition 0, 50, 75 and 100%. Soybean protein hydrolysate (SPH) was prepared by gel filtration chromatography using Sephadex G-25 to separate peptide based on molecular weight. Characterization of SPH and SPH fractions was also conducted, such as protein concentration, molecular weight, iron-binding capacity, and amino acid concentration. Iron binding capacity was determined by the colorimetric method using an ortho-phenanthroline reagent. The result showed that soybean protein hydrolysate increases iron-binding capacity 25-37 times than ascorbic acid. Arginine, aspartic acid, glutamic acid, threonine, lysine, and serine were found as amino acids responsible for iron-binding capacity in the SPH.
Co-Authors Erma Maryana Noer Laily Sri Peni Wijayanti