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All Journal Current Research on Biosciences and Biotechnology
Nilufar Nahar
Department of Chemistry, University of Dhaka
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Materials Science & Nanotechnology Medicine & Pharmacology

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Investigation of tetracycline residues in poultry meat samples from Dhaka city by high-performance liquid chromatography Nargis Parvin; Mohammad Shoeb; Nilufar Nahar; Rafiza Islam
Current Research on Bioscences and Biotechnology Vol. 3 No. 2 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.3.2/P89JJ95N

Abstract

The objective of the study was to quantify tetracycline (TCs) i.e., oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) residues in thirty poultry meat samples (n = 30) collected from the local market and super shop around the Dhaka University campus during May 2019 to January 2020. Three samples were collected from each of ten locations. All samples were extracted by Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method. Samples were analyzed by reversed-phase High-Performance Liquid Chromatograph equipped with Photo Diode Array Detector (LC-PDA) and a reported method was validated with good linear correlation coefficients of standards and matrix-matched calibration curves with r2=1.00, 0.99, 0.99 and r2= 0.99, 0.99,0.99 in the linearity range of 0-10 µg/kg for OTC, TC and CTC, respectively. The limit of detection (LOD) and limit of quantification (LOQ) for OTC, TC, and CTC were 1.05, 1.17, and 1.09 μg/kg and 3.15, 3.51 and 3.27 μg/kg, respectively. Accuracy that is expressed by the recovery percentages were calculated at two different concentrations (2.5 and 5 μg/kg) were 91 and 100%, 102 and 100%, and 106 and 100% for OTC, TC, and CTC, respectively. Intra-day (n=3) and inter-day (n=9, 3 days) precision data were under 10% for all sample matrixes. Standard deviations were calculated ±0.06, ±0.11 and ±0.03 and precision (expressed by RSD%) were found 5.57, 9.14 and 2.35%, respectively for OTC, TC and CTC. The HPLC-PDA method is affordable for screening of large number meat samples for residual antibiotics in biological matrices by any laboratories. The method is also cheaper in comparison with LC-MSMS. Analysis of real 30 poultry meat samples showed that the tetracyclines residues were below the quantification limit in all samples.
HPLC analysis of artificial preservatives, stimulants and sweeteners in carbonated beverages in Bangladesh Mohammad Shoeb; Md. Mafizul Islam; Md. Shahed Reza; Nilufar Nahar; Md. Mazharul Islam
Current Research on Bioscences and Biotechnology Vol. 3 No. 2 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.3.2/2V2BWDB5

Abstract

Different (n=21) commercial carbonated beverages, available in Bangladesh, were analysed by HPLC coupled with a photodiode array detector (UV-PDA). The main objective of this study was to quantify the amount of different artificial sweeteners, stimulant and preservatives and to validate the two methods used in this purpose. The two methods are simultaneous determination of sodium benzoate, caffeine, and saccharin using the sodium acetate buffer with pH 3.0 at 254 nm and simultaneous determination of artificial sweeteners acesulfame-K and aspartame using the potassium phosphate monobasic buffer. Correlation coefficient (r2) obtained were 0.9977, 0.9976, 0.9975, 0.9993 and 0.9956 in the range of 1-100 µ/L, recoveries were 95.88-97.10, 96.53-97.20, 94.53-96.05, 91.15-93.31 and 92.88-94.13%, LODs were 30.06, 15.46, 31.61, 0.33 and 0.83 mg/L, and LOQs were 100.18, 51.54,105.37, 1.11 and 2.77 mg/L for saccharin, caffeine, sodium benzoate, acesulfame-K and aspartame, respectively. The present analysis shows that amount of saccharin, caffeine, sodium benzoate, acesulfame-K and aspartame in the range of ND-377.60, ND-462.36, ND-217.60, ND-48.09 and ND- 295.20 mg/L, respectively. The total carbohydrate found as 102.81-147.16 g/L using the UV-visible spectrophotometric method which implies that sample containing more amount of carbohydrate has less artificial sweeteners value.
Co-Authors Md. Mafizul Islam Md. Mazharul Islam Md. Shahed Reza Mohammad Shoeb Nargis Parvin Rafiza Islam