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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Biotropika Indian Journal of Forensic Medicine & Toxicology
Rodiyati Azrianingsih
Biology Department, Mathematic and Natural Sciences Faculty, Brawijaya University, Malang,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Public Health

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Effect of Dietary Amorphophallus sp From East Java on LDL-C Rats (Rattus novergicus Wistar Strain) Nunung Harijati; Sri Widyarti; Rodiyati Azrianingsih
Journal of Tropical Life Science Vol. 1 No. 2 (2011)
Publisher : Journal of Tropical Life Science

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One of indication of obesity is high LDL-C . Obesity has serious risk to health, it can cause heart disease and stroke. Effort to lower obesity using drugs have significant side effects such as insomnia, increased blood pressure, dry mouth and so forth. Therefore using natural products that contain glucomannan to reduce obesity and LDL-C is good choise. Glucomannan in the global market derived from Amorphophallus konjac. In this study, we used glucomannan from Amorphophallus endemic East Java to reduce fattened Wistar rats. Amorphophallus that used include Amorphophallus muelleri, A.variabilis (variant: Brongkos 32; Brongkos 5; Wonorejo, Brangsi) and Amorphophallus campanulatus (Var Selopuro). Amorphophallus diet was given to white rats of Wistar strain that previously fattened using a mixture of cholesterol and lard, in addition to PARS (Chicken Feed Race Super) as a basic food. Amorphophallus konjac was used as control. Measurement of LDL-C was conducted in four different time points, namely 1). Prior to dietary cholesterol, 2). 24 days after the dietary cholesterol, 3) 11 days after Amorphophallus (+cholesterol) diet, 4). 25 days after Amorphophallus (+cholesterol) diet. The results showed that the diet of A.variabilis potentially lowered blood cholesterol levels for their respective 22.98%, 5.85% and 7.37% for consecutive variant Brongkos 32; Brongkos 5; Wonorejo. Diet from A.campanulatus and A.konjac had not been able to reduce cholesterol to the end of observation (25 days).Keywords: LDL-C, A.variabilis, A.muelleri, A.campanulatus, A.konjac
Ethno-Edible Mushroom of Pleurotus sp., Clytocybe nebularis and Auricularia auricula in Ranupani Village, East Java Jehan Ramdani Haryati; Rodiyati Azrianingsih
Journal of Tropical Life Science Vol. 2 No. 2 (2012)
Publisher : Journal of Tropical Life Science

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This paper documented the knowledge about the wild edible fungal flora of Ranupani Village of East Java Indonesia that used by indigenous Tenggerese tribes. Study was conducted by using semi-structured interview and purposive sampling. Data were analyzed quantitatively descriptive. Index of Cultural Significance (ICS) was used to evaluate the importance of non-wood forest yields for local people. Identification of edible mushrooms was based on Guidelines of Flora Diversity Data Collection. This study was focus on the three edible mushrooms which are eaten mostly by the villagers, i.e. Pleurotus sp., Clytocybe nebularis and Auricularia auricula. The result is the Ranupani residents perception of edible mushrooms and its potential as an alternative source of food based on local knowledge and local wisdom of Tenggerese. Pleurotus sp. has the highest preference and intensity to be consumed with 3,2 ICS. It means culturally, this edible mushroom were not too important but intensively used as a secondary food sources such as soup (added or sauted), chips (dried) and fried. Ranupani Villagers always consumed edible mushrooms in rainy season. The ability of residents to distinguish edible mushrooms are equal to the searching ability to find edible mushroom substrate. The residents will recognized the substrate of edible mushrooms on dead trunks of Pasang Tree (Lithocarpus sundaicus), Danglu (Engelhardia spicata), Kemlandingan (Albizia Montana), Casuarina (Casuarina junghuhniana) and acacia (Acacia decurens). Residents who do not have the ability to distinguish and searching were consumer, get the edible mushrooms by buying from the searcher or distributor in packs. The characteristics of an edible mushrooms are a discrete soft flesh of the fruit body, dark color, no ring on the stipe, the presence of insects (e.g. moths) in the lamellae) and the type of mushroom substrate. There were also assisted growths of wild mushrooms by the residents’ raw chop the dead tree trunk as a substrate deliberately. Respondents who knew the time of harvesting edible mushroom were 58.3%. Pasang mushrooms (natural & assisted growth) can be found throughout the rainy season, with an interval of 12 days for subsequent retrieval. While Barat and Kuping mushrooms are found throughout the rainy season, but randomly distributed in the forest. There were unwritten but spoken hereditary local wisdom for the sustainable of the edible mushrooms in Tengger. Only Tenggerese allowed collecting and selling the edible mushrooms in Tengger area to the Tenggerese themselves and tourist (local or foreign). This wisdom is supported by TNBTS regulation that the edible mushroom were not allowed exported out of TNBTS area. This wisdom expectedly makes sure that the edible mushrooms should be sustained in the forest. Keywords:Ethno-edible mushrooms, Ranupani
The Potency of Trichoderma sp. as A Biocontrol Agent against Fusarium sp. Pathogen of Porang (Amorphophallus muelleri Blume) Tuber Nindi Nazula Fajarini; Rodiyati Azrianingsih; Suharjono Suharjono
Journal of Tropical Life Science Vol. 10 No. 1 (2020)
Publisher : Journal of Tropical Life Science

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Porang (Amorphophallus muelleri Blume) has high economic value in Asia. Porang tuber is high in glucomannan and thus becomes one of the exportable food commodities. Porang plants are susceptible to wilt disease caused by Fusarium sp. Trichoderma sp. has the ability to inhibit the growth of pathogenic fungi such as Fusarium sp. This research aimed to study the potency of Trichoderma sp. in controlling the growth of Fusarium sp. Trichoderma sp. was isolated from the rhizosphere soil in A. muelleri plantation, while Fusarium sp. was isolated from A. muelleri tuber infected by Fusarium. The fungi isolates were identified phylogenetically based on the similarity of Internal Transcribed Spacers (ITS) sequence. Both fungi were antagonistically assessed based on the dual culture method. The antagonistic assay showed that the two isolates of Trichoderma had the potency to inhibit the growth of the two isolates of Fusarium. Trichoderma sp. 2 has higher antagonistic potency than Trichoderma sp. 1. Based on ITS sequence similarity, Trichoderma sp. 1 and Trichoderma sp. 2 were identified as Trichoderma longipale and Trichoderma spirale respectively, while both pathogenic Fusarium were identified as Fusarium oxysporum. Trichoderma spirale therefore could be developed as a biopesticide agent in controlling Fusarium oxysporum.
Co-Authors Anna Roosdiana Dita Fitriana Kusuma Dewi Estri Laras Arumingtyas Gustini Ekowati Jehan Ramdani Haryati Nindi Nazula Fajarini Nunung Harijati Serafinah Indriyani Siti Muslichah Sri Widyarti Suharjono Suharjono Wahyu Ririn Anggraeni Widya Artika Sari Yessica Kristi Natalia Yuni Septiani Yustino Armend Wigoeno