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All Journal Molecular and Cellular Biomedical Sciences (MCBS)
Fahim Halim Khan
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh
Biochemistry, Genetics & Molecular Biology Dentistry Immunology & microbiology Medicine & Pharmacology Neuroscience

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Photo-illuminated Glutathione Inactivates Alpha-2-macroglobulin: Spectroscopic and Thermodynamic Studies Syed Saqib Ali; Haseeb Ahsan; Sana Ansari; Khan M Abdullah; Fahim Halim Khan
Molecular and Cellular Biomedical Sciences Vol 6, No 1 (2022)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v6i1.223

Abstract

Background: Glutathione (GSH) is a principle thiol-containing tripeptide (cysteine, glutamic acid and glycine) antioxidant against free radicals and other harmful oxidants in cellular defence. The alpha-2-macroglobulin (α2M) is large tetrameric zinc-binding glycoprotein which inhibits proteinases regardless of their specificity and catalytic mechanism.Materials and Methods: The interaction of GSH was analyzed with α2M including the structural and functional alterations in α2M using various biochemical and biophysical methods. UV-visible and fluorescence spectroscopy were used to study the binding of α2M with GSH and Fourier transform infrared (FT-IR) spectroscopy was explored to study the structural change induced in α2M.Results: The results suggest that exposure of α2M to GSH decreases the antiproteolytic potential as suggested by the amidase assay. The UV-spectroscopic study showed the formation of α2M-GSH complex and fluorescence analysis showed significant quenching in fluorescence intensity of α2M suggesting GSH binding and structural alteration in the protein. FT-IR spectroscopy was explored to study the structural change induced in α2M which suggest that the secondary structure of α2M changes upon complex formation.Conclusion: Our studies show that interaction of α2M with photoilluminated GSH results in functional and conformational changes of the protein.Keywords: glutathione, GSH, alpha-2-macroglobulin, photo-illumination, ITC, FTIR
Interaction of Silver Nanoparticles with Human Alpha-2-macroglobulin: Biochemical and Biophysical Investigation Tooba Siddiqui; Mohammad Khalid Zia; Mohammad Muaz; Haseeb Ahsan; Fahim Halim Khan
Molecular and Cellular Biomedical Sciences Vol 6, No 3 (2022)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v6i3.281

Abstract

Background: Silver nanoparticles (AgNPs) have a broad spectrum of applications in nanoscience and nanomedicine due to their flexible properties, such as antibacterial, antifungal, anti-inflammatory and anti-angiogenic. Present study investigated the interaction of chemically synthesized AgNPs with human major antiproteinase alpha-2-macroglobulin (α2M).Materials and methods: The first step of the study involved the synthesis and characterization of AgNPs using various biochemical and biophysical techniques, such as UV-visible spectroscopy, fluorescence quenching spectroscopy, synchronous fluorescence, and circular dichroism (CD). Different methods were used to explore the primary and secondary structural changes induced in α2M by the binding of AgNPs. Results: The UV-visible spectroscopy revealed hyperchromicity in the absorption spectra of α2M. The presence of a static quenching mechanism was indicated by the temperature-dependent fluorescence spectroscopy. The synchronous fluorescence revealed a change in the microenvironment of the tryptophan residues in α2M. The CD results showed the reduction in β-helical content of α2M. The activity of α2M decreased significantly with the increase of AgNPs concentration.Conclusion: Our result suggests that AgNPs cause modifications in the structure and functional activity of α2M. The interaction of nanoparticles with proteins is important for understanding their potential risks to human health. Keywords: alpha-2-macroglobulin, antiproteinase, silver nanoparticles, fluorescence quenching, FTIR, TEM
Co-Authors Haseeb Ahsan Khan M Abdullah Mohammad Khalid Zia Mohammad Muaz Sana Ansari Syed Saqib Ali Tooba Siddiqui