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Siti Aminah Sulaiman
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Mathematics

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MEDIUM OPTIMIZATION FOR THE PRODUCTION OF BIOMASS BY Cunninghamella sp. 2A1 USING RESPONSE SURFACE METHODOLOGY Siti Aminah Sulaiman; Aidil Abdul Hamid; Wan Mohtar Wan Yusoff
ASEAN Journal on Science and Technology for Development Vol. 22 No. 3 (2005): ASEAN Journal on Science and Technology for Development (AJSTD)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (189.532 KB) | DOI: 10.29037/ajstd.160

Abstract

A statistical design approach has been used to optimize the production of biomass byCunninghamellasp. 2A1, evaluated based on lipidless biomass. A 23full factorial centralcomposite design (CCD) was chosen to study the combined effects of three factors; ammoniumtartrate, peptone and glucose concentrations. The p-value for each factors was < 0.05 suggestingthat these factors have significant effect on the production of lipidless biomass. The productionis represented by a linear model with p-value < 0.0001. The optimized medium consists of 3.86g/l ammonium tartrate, 55.84 g/l glucose and 7.73 g/l peptone predicted 16.83 g/l lipidlessbiomass. Results from four replications based on the optimized medium produced 18.48 g/llipidless biomass, which are in close agreementwith the predicted value. The coefficient forglucose was the highest indicating it as the most significant factoraffecting lipidless biomassproduction. 
Co-Authors Aidil Abdul Hamid Wan Mohtar Wan Yusoff