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Nguyen Hong Thanh
Institute of Biotechnology, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet Rd., Cau Giay Dist., Hanoi
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Mathematics

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STUDIES ON A NOVEL RECOMBINANT LIPASE A FROM BACILLUS SUBTILIS FS2 Phung Thu Nguyet; Nguyen Hong Thanh; Truong Nam Hai; Jan-Christer Janson
ASEAN Journal on Science and Technology for Development Vol. 25 No. 2 (2008): ASEAN Journal on Science and Technology for Development (AJSTD)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.029 KB) | DOI: 10.29037/ajstd.263

Abstract

A strain of Bacillus subtilis FS2 isolated from a traditional fish source in Vietnam has been shown to produce an enzyme possessing both a gelatinase and a lipase A (LipA) activity when tested on agar plates containing 0.1% tributyrin and 0.3% gelatine. A gene encoding LipA  was isolated and expressed in E. coli BL21 (DE3). The enzyme has got an apparent Mr of 24 kDa, a pI of 9.2 and was purified to electrophoretical homogeneity by affinity chromatography. Both the lipase and the gelatinase showed optimum activity at pH 10 and at 30 0 C, with highest activity in the range 25-37°C. The metal ions Fe++, Co++, and Mn++ at 10 mM had strong inhibitory effect on both activities whereas Ca++ had no effect and Zn++  had a slight enhancing effect. The specific enzymatic activities obtained were 19814 U/mg for the lipase and 1245 U/mg for the gelatinase.  
Co-Authors Jan-Christer Janson Phung Thu Nguyet Truong Nam Hai