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Sendy B Rondonuwu
Jurusan Biologi Fakultas MIPA Universitas Sam Ratulangi Manado
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Isolasi dan Identifikasi Bakteri yang Toleran terhadap Fungisida Mankozeb pada Lahan Pertanian Tomat di Desa Tempok, Kecamatan Tompaso, Sulawesi Utara (Isolation and Identification Mankozeb Fungicide-Tolerant on the Tomato Farm in Tempok Village, Tompaso Lumantouw, Semuel F.; Kandou, Febby EF; Rondonuwu, Sendy B; Singkoh, Marina F.O.
JURNAL BIOS LOGOS Vol 3, No 2 (2013): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.3.2.2013.4433

Abstract

AbstrakPenggunaan fungisida mankozeb secara terus-menerus untuk menangani penyakit yang disebabkan jamur dapat mengakibatkan kerusakan pada lingkungan dan mengganggu kesehatan. Penelitian ini bertujuan untuk mengisolasi dan indentifikasi bakteri yang toleran terhadap fungisida mankozeb yang terdapat pada lahan pertanian tomat di Desa Tempok Kecamatan Tompaso. Penelitian dilakukan dengan cara mengisolasi bakteri pada medium seleksi yang mengandung fungisida mankozeb 10% selama 2 hari pada suhu 37ËšC kemudian dimurnikan dengan menggunakan medium seleksi yang sama. Setelah didapatkan isolat murni, uji morfologi dan uji biokimia dilakukan. Uji morfologi dan biokimia serta identifikasi menunjukkan ada 3 genus bakteri yang toleran terhadap fungisida mankozeb, yaitu Vibrio sp., Mycobacterium sp., dan Corynebaterium sp.Kata kunci : koloni, bakteri, fungisida mankozebAbstractThe continuous use of mankozeb fungicide to treat diseases caused by fungi can result in the damage of the environment and the community health. This study aimed to isolate and identify the bacteria that are tolerant to mancozeb on the tomato farms in the Tempok Village , Tompaso District. This study was carried out by isolating the bacteria on the selection medium containing 10% mancozeb for 2 days at 37ËšC and then purifying them using the same medium. After obtaining the pure isolates, the morphological and biochemical tests were conducted. The morphological and biochemical tests as well as the identification indicated that there were 3 genera of mancozeb-tolerant-bacteria,i.e. Vibrio sp., Mycobacterium sp., and Corynebacterium sp.Keywords : colony, bacteria, mancozeb fungicide
Identification of Mangrove using DNA Barcoding and Vegetation Analysis at the Sarawet Mangrove Ecotourism Site, North Minahasa Regency Rondonuwu, Sendy B; Maabuat, Pience; Kamu, Vany; Pujiastuti, Dwi Rahayu
JURNAL BIOS LOGOS Vol. 16 No. 1 (2026): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v16i1.67260

Abstract

This study aims to identify the diversity of mangrove species in the Mangrove Ecotourism area of Sarawet Village, Likupang, North Minahasa Regency, using a molecular taxonomy approach based on DNA barcoding and an ecological approach through vegetation analysis. Vegetation data collection was conducted descriptively and qualitatively using transect and plot methods. Molecular analysis included DNA extraction from mangrove leaf samples, amplification of the matK and rbcL genes using Polymerase Chain Reaction (PCR), followed by sequencing and nucleotide base comparison using the Basic Local Alignment Search Tool (BLAST). The morphological vegetation analysis found six mangrove species at the research site, namely Rhizophora mucronata, Rhizophora apiculata, Bruguiera sp., Sonneratia alba, Aegiceras corniculatum, and Ceriops tagal. Meanwhile, the molecular identification results of DNA barcoding showed that the matK gene sequence in the SRA sample had a 99.98% similarity with Sonneratia alba, and the rbcL gene sequence in the SRC sample had a 100% similarity with Aegiceras corniculatum. The combination of molecular identification and vegetation analysis provides accurate data on mangrove species composition, which is highly essential as a foundation for monitoring, sustainable management, and conservation of the mangrove ecotourism area as a buffer zone.
Co-Authors Febby EF Kandou Kamu, Vany Maabuat, Pience Marina F. O. Singkoh Semuel F. Lumantouw