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PENGEMBANGAN METODE UJI SARING LIMA VARIETAS KACANG HIJAU (Vigna radiata) TAHAN KERING Nafila Alifia Azka
Agrotechnology Innovation (Agrinova) Vol 4, No 1 (2021)
Publisher : Agrotechnology Innovation Center, Universitas Gadjah Mada, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/a.74380

Abstract

Mungbean (Vigna radiata) is one of the most widely cultivated legume in the world after soybeans and peanuts. Mungbean is easy to cultivate, but the facts in the field state that domestic mungbean production in Indonesia is still very low. One of the abiotic stress factors that often cause low mungbean production is drought, so it is necessary to develop a screening test method for five drought-resistant mungbean varieties, Kutilang (control/V1), Sriti (V2), Vima (V3), Murai ( V4), and Kenari (V5). Screening tests were carried out on the germination and vegetative phases using Polyethylene Glycol (PEG) 6000 with a dose of 0% PEG 6000 as control (K0), 5% PEG 6000 (K1), and 10% PEG 6000 (K2). Based on the results of the screening test of five mungbean varieties using PEG 6000, the germination phase was the most appropriate phase for a drought screening test. In the germination phase drought screening test, the varieties that were resistant to drought stress were Kenari, Sriti, Murai, and Vima, while those that were intolerant was Kutilang. In the vegetative phase, the most resistant variety was Kenari.
Perkecambahan Polen Bunga Jengger Ayam (Celosia argantea) Nafila Alifia Azka
Agrotechnology Innovation (Agrinova) Vol 4, No 2 (2021)
Publisher : Agrotechnology Innovation Center, Universitas Gadjah Mada, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/a.77012

Abstract

Celosia cristata is one of specialty cut flowers category. Celosia cristata has leaf color, leaf shape and stem color diversity. The diversity in Celosia cristata has attracted the attention of breeders to develop new varieties of this commodity by means of hybridization. Therefore, before the hybridization of Celosia cristata is being conducted, it is necessary to study the estimated time required for pollen to reach the embryo sac and fertilization occurs to ensure the probability of successful hybridization in plant breeding program. Pollen germination in this study was carried out by in vitro method using pollen germination media consisting of 12 grams of sucrose, 0.01 grams of H3BO3, 100 ml of distilled water, and 0.1 M HCl or 0.1 M NaOH (to maintain the pH of the media at pH 6.4). The germinated pollen was observed with a microscope until the germination reed reached its maximum length, then the length of the germination reed was measured. The measured growth of germination reeds was then compared with the length of the distance from the stigma to the embryo sac. After that, the estimated time required from pollination to fertilization was being predicted. Based on the results of the study, the estimated time needed by Celosia cristata from pollination (pollen fall to the stigma) to fertilization (fusion between male and female gamete cells) was 131.9 minutes.