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KUALITAS BIJI DAN KARAKTER AGRONOMI PADI HITAM (Oryza sativa L.) LOKAL PEKALONGAN DENGAN PENAMBAHAN PAKLOBUTRAZOL DAN GIBERELIN Endang Triyani Prihantari; Triani Hardiyati; Siti Samiyarsih
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4240

Abstract

Black rice (Oryza sativa L.) is a rice variety that has many additional functions and is used as a functional food. However, black rice has a longer harvest period and has a higher apparent stem height than other types of rice so that black rice plants are easy to break. This will reduce the quality of rice plants. One of the methods that can be done to overcome this problem is treating by paclobutrazol and gibberellin. The combination of paclobutrazol and gibberellin is expected to provide better rice growth and improve the quality of rice seeds. The purpose of this study was to determine the effect of the addition of paclobutrazol and gibberellin on seed quality, palea-lemma size and agronomic character of local Pekalongan black rice (Oryza sativa L.). The research was conducted experimentally using a completely randomized design (CRD) of factorial treatment pattern. The first factor was paclobutrazol with 4 levels of concentration (0 ppm, 100 ppm, 200 ppm and 300 ppm) applied once at 4 weeks of plant age and the second factor was gibberellin with 4 levels of concentration (0 ppm, 100 ppm, 200 ppm and 300 ppm ) applied once at the age of 6 weeks of plants, so that are 48 combinations with each treatment was replicated 3 times. The parameters observed included plant height, leaf width, flowering age, harvest age, palea-lemma size, weight of 100 seeds, protein and amylose content. The data obtained were analyzed using the ANOVA test at the 95% confidence level, and continued with the Honestly Significant Difference (HSD) test at an error rate of 5%.The results showed that the interaction between paclobutrazol and gibberellin was not significant, but treatment of 300 ppm paklobutrazol showed the best results in plant height inhibition, while the treatment of paclobutrazol 200 ppm was the best concentration to stimulate flowering and harvesting age. Treatment of gibberellin 100 ppm was found the best concentration to increase leaf width while a concentration of 300 ppm proved to give the best results for palea-lemma size, amylose content, protein content and weight of 100 seeds per plant.
Cross-tolerance Responses of Gamma Co60 Irradiation-derived Sugarcane Mutants to Salinity and Osmotic Stress Under In Vitro Conditions Gieks Sugiyono; Annaasta Tria Nur Alpyan; Nesya Diana Prameswari Putri; Siti Samiyarsih; Paul A. Davison; Rendie Prasetyo
Al-Kauniyah: Jurnal Biologi Vol. 19 No. 2 (2026): AL-KAUNIYAH: JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v19i2.51294

Abstract

Sugarcane (Saccharum officinarum L.) cultivation is increasingly expanding into marginal lands affected by salinity and drought stress, requiring the development of stress-tolerant cultivars. This study aimed to evaluate the cross-tolerance responses of gamma irradiation-derived sugarcane mutants to salinity and osmotic stress under in vitro conditions. The experiment was arranged in a split-plot design with three replications. The main plot consisted of two putative mutant clones (11.201 and 11.401) and one wild-type clone (11.000) of sugarcane cultivar NXI 1–3. Salinity stress was induced using NaCl concentrations of 0, 50, 100, 150, 200, and 250 mM, while osmotic stress was induced using polyethylene glycol (PEG) concentrations of 0, 5, 10, 15, 20, and 25%. Observed parameters included explant survival percentage and the numbers of shoots, leaves, and roots. Both NaCl and PEG treatments significantly reduced microshoot growth. However, clone 11.201 consistently showed better survival and growth performance under both stress conditions compared with the wild type and clone 11.401. Clone 11.201 maintained its growth under 200 mM NaCl, yielding an average of 33.67 ± 13.58 shoots/explant, 143.00 ± 61.51 leaves/explant, and 2.33 ± 2.08 roots/explant. It also exhibited limited leaf production at 15% PEG (3.33 ± 5.77 leaves/explant.  The results indicate the potential occurrence of cross-tolerance associated with gamma irradiation-induced variation.