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All Journal Indonesian Aquaculture Journal International Journal of Agriculture System

Andi Parenrengi

Research Institute for Coastal Aquaculture, Maros

Author-ID : 3672414

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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RAPD FINGERPRINTING OF THREE SPECIES OF GROUPER (Epinephelus spp.) FROM MAKASSAR STRAIT, SOUTH SULAWESI, INDONESIA Andi Parenrengi
Indonesian Aquaculture Journal Vol 1, No 2 (2006): (December 2006)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Grouper belonging to the epinepheline serranid family has promising aquaculture potential for food and ornamental fish production. Random amplified polymorphic DNA (RAPD) technique was employed in this study to determine the genetic variability and species differentiation of groupers from genus Epinephelus (E. areolatus, E. merra, and E. suillus). Groupers (N=52) were collected from Makassar Strait, South Sulawesi, Indonesia. Of 34-screened RAPD primers, ten primers (OPA-02, OPA-06, OPA-08, OPA-10, OPA-15, OPA-16, OPA-17, OPA-18, OPA-19, and CA-05) were selected to generate the RAPD fingerprinting of groupers. Results indicated that the ten primers revealed different RAPD profile among three species. Similarity index obtained by pairwise comparison was 0.62 ± 0.07 for E. areolatus, 0.58 ± 0.11 for E. merra and 0.80 ± 0.11 for E. suillus. Polymorphism and genotype number detected were 51.3% ± 8.7% and 3.8 ± 0.6 in E. areolatus; 56.8% ± 14.6% and 4.1 ± 1.1 in E. merra; and 60.2% ± 11.8% and 3.8 ± 0.9 in E. suillus, respectively. Genetic distance level of individuals ranges from 0.19—0.42, 0.14—0.50, and 0.02—0.30 for E. areolatus, E. merra, and E. suillus, respectively. The genetic distance between E. merra and E. areolatus (D=0.52) was closer compared with E. merra and E. suillus (D=0.67). A total of 33 RAPD markers differentiating the three species of groupers were detected. The study presents the RAPD technique as the useful genetic marker for assessment of genetic diversity and species differentiation in groupers.

CLONING OF ProAV PROMOTER ISOLATED FROM TIGER PRAWN Penaeus monodon Andi Parenrengi; Alimuddin Alimuddin; Sukenda Sukenda; Komar Sumantadinata; Muhammad Yamin; Andi Tenriulo
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Promoter is a specific DNA sequence involved in the transcription of a particular gene. It is usually located in the upstream of the gene they regulate. Isolation and characterization of promoter is essentially needed in order to establish the sequence analysis and transcription factor that are used in the regulation of gene expression. The research was conducted to analyze the characteristics of Penaeus monodon anti viral gene promoter (ProAV) towards generation of auto-transgenic tiger prawn, P. monodon. ProAV promoter was isolated by PCR (Polymerase Chain Reaction) method and the purified DNA fragment was cloned into pGEM-T Easy cloning vector. The promoter sequence was characterized by using BLAST-N and Genetyx version 7 softwares. The results showed the success in isolating a promoter from tiger prawn of 368 bp in length. BLAST-N analysis showed that the sequence of isolated promoter has high similarity (95%-98%) compared to the other promoters in the GeneBank. The study revealed the existence of important transcription factors (TATA box, MRE, TCF-1, and other potential regulatory elements) are identified in the promoter sequence.

GENETIC VARIABILITY AND POPULATION STRUCTURE OF GROUPER (Epinephelus suillus) FROM MAKASSAR STRAIT AND BONE BAY, SOUTH SULAWESI, INDONESIA Andi Parenrengi; Andi Tenriulo
Indonesian Aquaculture Journal Vol 3, No 2 (2008): (December 2008)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Random Amplified Polymorphic DNA (RAPD) was employed to determine the genetic variability and population structure of grouper (Epinephelus suillus) from Makassar Strait and Bone Bay, South Sulawesi, Indonesia. Genomic DNA was isolated from preserved muscle tissue using Phenol-Chloroform technique. Among 24-screened arbitrary primers, ten primers (OPA-02, OPA-06, OPA-08, OPA-10, OPA-15, OPA-16, OPA-17, OPA-18, OPA-19 and CA-05) were selected to generate RAPD fingerprinting of grouper populations. The ten primers generated a total of 212 fragments (loci) and 120 polymorphic fragments in their size ranging from 250 to 2,500bp. The high polymorphism (60%) was obtained from Makassar population followed by Bone (59%) and Pare-Pare populations (50%). Similarity index of individuals was 0.86±0.07 for Pare-Pare, 0.80±0.11 for Makassar and 0.82±0.07 for Bone population. Fifteen fragments from ten primes were identified as species-specific markers of E. suillus. The UPGMA cluster analysis showed that the dendrogram seemed to be clustered according to their geographical location, where Pare-Pare population was genetically closer to Makassar population (D=0.20) than to Bone population (D=0.24).

GENETIC, COLORATION, AND GROWTH PERFORMANCE OF TWO DIFFERENT VARIETIES OF Kappaphycus alvarezii Sulaeman Sulaeman; Andi Parenrengi; Emma Suryati; Rosmiati Rosmiati
Indonesian Aquaculture Journal Vol 2, No 1 (2007): (June 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Two different colors (green and brown) of Kappaphycus alvarezii have been farmed in Indonesian waters for many years. This study aimed at comparing two ‘varieties’, i.e. green and brown, both genetically and morphologically. Samples for DNA analysis were collected from a farmer in Pinrang Regency, South Sulawesi. Five universal primers i.e. Ca-01, Ca-02, P-40, P-50, and DALRP were selected to obtain DNA genetic markers in differentiating the green and brown varieties. To compare coloration patterns during cultivation and the growth performance of both varieties, a field experiment was performed in a seaweed farming area in Pinrang Regency, during dry season of August-September 2004. The result of genetic assessment showed that the five selected primers revealed different RAPD banding pattern for both varieties. P-50 and DALRP primers demonstrated the greatest amplification in differentiating RAPD fragment between green and brown varieties. Fragment 900 bp and 1.300 bp were consistently generated in the green variety but were not amplified in the brown variety. The result of the field study confirmed that the coloration pattern of green and brown varieties was fixed; no interchange in color occurred during one crop cultivation.

Crablet of Mud Crab Scylla Olivacea Production from the Different Stages of Larvae fed Artemia Nauplii Enriched Using Nannochloropsis sp Gunarto Gunarto; Andi Parenrengi; Early Septiningsih
International Journal of Agriculture System VOLUME 4 ISSUE 2, DECEMBER 2016
Publisher : Hasanuddin University

Improvement of feed quality for mangrove crab larvae rearing is one of theimportant factors to increase of crablet production. The aim of the research was to knowthe influenzing of enriched Artemia nauplii using Nannochloropsis sp fed to the differentstages of larvae Scylla olivacea on crablet production. Twelve units of tanks volume 250L filled with saline water salinity 30 ppt, aerated, then stocked with new hatched mud crablarvae zoea-1 at the density 100 ind./L. The larvae zoea-1 were fed rotifer, Brachionus sp.and after zoea-3, beside fed by rotifer, the larvae were also fed by Artemia naupli. Fourtreatments were tested, namely: A). Artemia nauplii enriched using Nannochloropsis sp.was given to the larvae zoea-3 until develop to megalop stage. B). Artemia nauplii enrichedusing Nannochloropsis sp was given to the larvae zoea-4 until develop to megalop stage.C). Artemia nauplii enriched using Nannochloropsis sp was given to the larvae zoea-5 stageuntil develop to megalop stage. D). Artemia nauplii without enriched using Nannochloropsissp. was given to the larvae zoea-3 until develop to megalop stage. Result of the researchshowed that the highest of Larvae Development Indexes and Megalop Occurence Indexeswas obtained in treatment A and resulted the highest of crablet production, then followed bytreatment C and B and those were significantly different (P<0.05) with the crablet productionin treatment D. The use of Artemia nauplii enriched by Nannochloropsis sp. to feed larvae,S. olivacea stage zoea-3 to zoea-5 until develop to the megalop stage is one of the key factorto enhance the crablet production. By this finding, the crablet production in hatchery will beincreased and the mud crab culture in brackishwater pond able to developed.

Co-Authors Alimuddin Alimuddin Andi Tenriulo Early Septiningsih Emma Suryati Gunarto Gunarto Komar Sumantadinata Muhammad Yamin Rosmiati Rosmiati Sukenda Sukenda Sulaeman Sulaeman

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