Ishaaq Saputra
Fish Quarantine and Inspection Agency Regional Office Jakarta I

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THE EFFECTS OF TRANSPORTATION ON IMMUNE MODULATION OF WILD AND OCEAN-RANCHED GREENLIP ABALONE (Haliotis laevigata) Sri Rahayu Setyaningsih; Ravi Fotedar; Roy Melville-Smith; Ishaaq Saputra; Nelson Fernandez; Subas Adhikari
Indonesian Aquaculture Journal Vol 15, No 1 (2020): (June, 2020)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.15.1.2020.33-41

Abstract

The effects of live transportation on the immune modulation of wild and ocean-ranched greenlip abalone (Haliotis laevigata) were evaluated. Samples of abalone were collected both in autumn and winter in Flinders Bay, Augusta, Western Australia, and land transported for 6 (six) hours. Several immunological parameters were assessed, including survival rate, total haemocyte count, osmoregulatory capacity, phagocytic rate, lactate level, and glucose level. The results indicated that the abalone samples collected in the two seasons showed different physiological responses; the winter samples were more responsive to transportation than the autumn samples. Transportation stress significantly increased total haemocyte count and osmoregulatory capacity of the winter samples, suggesting an immune stimulation. This stress also triggered an immune suppression, causing the phagocytic rate and lysosomal stability to significantly decrease after transportation. Lactate levels in the winter samples decreased significantly after transportation, possibly indicating the transition from a stressed to normal state, during the period of recovery. The constant level of glucose before and after transportation in both seasons showed that it was the least sensitive indicator used in this study. In general, there was no difference in the immune modulation parameters between wild and ranched abalone in either of the seasons sampled. However, in the autumn samples, there were significant differences (P<0.05) in haemocyte count and osmoregulatory capacity of the wild and ranched samples collected from all sites. These differences did not form a consistent indicator trend between the sites from the two sample sources. Therefore, the results do not raise any immediate concern that ranched abalone were differently stressed to those from the wild. The results in present study could serve as useful data in developing the live transportation method of greenlip abalone.
Growth Performance of Smooth Marron (Cherax cainii) Fed Different Dietary Protein Sources Ishaaq Saputra; Ravi Fotedar
Journal of Aquaculture and Fish Health Vol. 10 No. 1 (2021): JAFH Vol 10 No. 1 February 2021
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v10i1.20794

Abstract

Fish farming including freshwater crayfish still has a dependency on the availability of fish meal as the main source of protein in feed. The purpose of this study was to evaluate alternative protein sources including poultry by-products, feather meal, lupine flour, soybean meal and meat, and bone meal for smooth marron (Cherax cainii) freshwater crayfish feed. A total of 150 juvenile marrons (9.09 ± 0.21 g) were kept for 90 days and distributed into 15 rearing tanks consisting of five treatments with three replications. The results indicated that there was no significant difference in the increase in biomass, survival rate, moulting rate (weight), feed efficiency ratio, and feed digestibility (P> 0.05). However, marron juvenile fed with a protein source of poultry by-products meal had the highest specific growth rate (0.31 ± 0.05 g / day). In addition, marron fed with fish meal protein resulted a significant increase in carapace length (P <0.05). Based on the digestibility test, it was found that the digestibility level of the feed ranged from 76.39 ± 0.01 - 79.11 ± 0.01% and replacement of fish meal had no significant effect on dry matter digestibility (P> 0.05). Overall, the results of this study indicate that the general growth performance of marron is not affected by the replacement of fish meal in the feed. Alternative protein materials can be used as a protein source to replace fish meal in marron feed so that the use of fish meal can be reduced.