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All Journal Indonesian Aquaculture Journal

Ketut Sugama

Center for Aquaculture Research and Development, Jakarta

Author-ID : 3672582

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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IDENTIFICATION OF A LOCAL PROBIOTIC BACTERIUM USING 16S rRNA GENE SEQUENCE THAT WAS USED FOR FIELD TRIAL TO ENHANCED WHITELEG SHRIMP (Litopenaeus vannamei) SURVIVAL Tb. Haeru Rahayu; Ketut Sugama
Indonesian Aquaculture Journal Vol 10, No 2 (2015): (December 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The use of local probiotics in the culture of aquatic organisms is increasing with the demand for more environmental-friendly aquaculture practices. The local bacterium isolate considered as a probiotic was added into the water of whiteleg shrimp (Litopenaeus vannamei) culture in a field trial. Four rectangular plastic ponds (ca. 20 m x 30 m per pond) were used for 100 days experimentation for six consecutive crops in two years experiment. Survival, harvest size, feed conversion ratio (FCR) and Vibrio bacterial count was compared with those of shrimp receiving and none of local isolate. Identification based on 16S rRNA gene sequence shown those isolate was Bacillus pumilus strain DURCK14 with 99% homology. Water shrimp pond added a local isolate had significantly higher survival at about 10.0% to 11.7% than shrimp without added the isolate (p<0.05), and better FCR, but no significant different in shrimp harvest size. Vibrio bacterial was undetected by total plate count. Moreover, it shown better projected yields on an annual basis (three crops per year).

FREQUENT MONITORING OF WATER TEMPERATURE IN PEGAMETAN BAY, BALI: A PRELIMINARY ASSESSMENT TOWARDS MANAGEMENT OF MARINE AQUACULTURE DEVELOPMENT I Nyoman Radiarta; Erlania Erlania; Ketut Sugama; Hirmawan Tirta Yudha; Masaaki Wada
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Aquaculture currently share for nearly half of the world’s food fish consumption, and continue to be the fastest-growing animal food producing sector. The viability of aquaculture operation has greatly been affected by the characteristic of marine environment. Inventory and monitoring of marine environment are necessary and can be done through information technology implementation. Frequent monitoring of water temperature, for almost one year observation, at four aquaculture sites in Pegametan Bay and Research and Development Institute for Mariculture was investigated. Water temperature data were obtained by using logger and buoy systems. These data were contrasted against marine fish mortality. On the other hand, the suitability of species requirements with the thermal conditions was evaluated by comparing temperature range to the optimum and lethal temperature information available on marine fish species of aquaculture interest. This research could be beneficial for enhancing productivity of marine aquaculture operation in terms of possible impact of climate change. It was also possible to find the ideal temperature range for culturing fish species, taking into account the variability associated with large-scale phenomena.

FLUCTUATING ASYMMETRY REFLECT THE GROWTH OF HYBRID GROUPER Epinephelus fuscoguttatus AND Epinephelus polyphekadion Ketut Sugama; Ahmad Muzaki; I Gusti Ngurah Permana; Haryanti Haryanti
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Fluctuating asymmetry has been widely used as a measure of developmental stability and as an indicator of individual fish growth. The present study compared fluctuating asymmetry in three bilateral meristic traits of F-1 hybrid between female Epinephelus fuscoguttatus and male Epinephelus polyphekadion and two F-1 pure parental progenies. The fishes were reared by communal and separate tank systems. Hybrids were confirmed by allozymes electrophoresis. After three months of rearing, the F-1 hybrids fish grew faster 45.9% and 66.6% compare to the F-1 pure parental progenies of E. fuscoguttatus and E. polyphekadion (P<005) respectively. Development stability as measured by fluctuating asymmetry was lower in hybrid (20%-40%) than in the pure parental progenies (53%-80%). Among four enzyme loci examined, only Pgm locus showed variants and at that locus all hybrids fish were heterozygous. Heterozygous hybrids showed lower fluctuating asymmetry and related to higher developmental stability. The higher developmental stability positively reflected a faster growth rate. Thus this finding can be used to explain the reason that why hybrids groupers appeared grew faster than parental species.

Co-Authors Ahmad Muzaki Erlania Erlania Haryanti Haryanti Hirmawan Tirta Yudha I Gusti Ngurah Permana I Nyoman Radiarta Masaaki Wada Tb. Haeru Rahayu

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