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All Journal Indonesian Aquaculture Journal
Isti Koesharyani
Center for Aquaculture Research and Development, Jakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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DETECTION OF Macrobrachium rosenbergii Nodavirus (MrNV) AND EXTRA SMALL VIRUS (XSV) DISEASES ON GIANT FRESHWATER PRAWN, Macrobrachium rosenbergii AT SAMAS, YOGYAKARTA Isti Koesharyani; Lila Gardenia
Indonesian Aquaculture Journal Vol 9, No 1 (2014): (June 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1205.937 KB) | DOI: 10.15578/iaj.9.1.2014.33-40

Abstract

Mass mortality of giant freshwater prawn (Macrobrachium rosenbergii de Man) in grow-out farmers occurred in early February 2012 at Instalation Coastal of Aquaculture Samas, Bantul, D.I. Yogyakarta. The clinical sign of shrimp was whitish coloration on abdominal and tail muscle. The sympton was the same as in other cases of mortality caused by prawn Macrobrachium rosenbergii nodavirus (MrNV) and Extra Small Virus (XSV). Prawn samples were diagnosed by standard protocols Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) using specific primers and histopathology analysis. The result showed that all samples indicated positive 13/15 the MrNV and 5/15 positive XSV, and there were 4/15 positive samples both (MrNV and XSV). Analysis of histopathology showed that damaged muscle was indicated by the presentation of necrotic tissues with nuclear pyknosis or degeneration of muscle in infected tissues. Based on diagnosis by RT-PCR and histopathological, mass mortality of the giant freshwater prawn in Indonesia is determined to be caused by “white muscle disease (WMD)/white tail disease (WTD)”.
DEVELOPMENT OF PRIMARY CELL CULTURE FROM TAIL EPIDERMAL TISSUE OF KOI CARP (Cyprinus carpio koi) Lila Gardenia; Isti Koesharyani
Indonesian Aquaculture Journal Vol 9, No 1 (2014): (June 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (480.805 KB) | DOI: 10.15578/iaj.9.1.2014.41-50

Abstract

Primary cell culture from tail epidermal tissue of koi carp (Cyprinus carpio koi) was developed. Cells were grown in Leibovits-15 medium supplemented with 20% fetal bovine serum and antibiotics (Penicillin/Streptomycin and Kanamycin). Cell growth was observed in a range of incubation temperature (17oC±2oC, 22oC±2oC, 27oC±2oC, and 32oC±2oC) in order to determine the optimum temperature. The cells were able to grow at a range of temperature between 17oC to 32oC with optimal growth at 22oC. Primary cells infected with koi herpes virus produced typical cytopathic effects characterized by severe vacuolation and deformation of nuclei, which is consistent with those of previous reports. Artificial injection experiment by using supernatant koi herpes virus SKBM-1 isolate revealed that it could cause 90% mortality in infected fish within two weeks. PCR test with Sph I-5 specific primers carried out with DNA template from supernatant virus, pellet cell, and gills of infected fish showed positive results in all samples (molecular weight of DNA target 290 bp). The cells were found to be susceptible to koi herpes virus and can be used for virus propagation.
Co-Authors Lila Gardenia