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All Journal Indonesian Aquaculture Journal Jurnal Riset Akuakultur
Samuel Lante
Balai Penelitian dan Pengembangan Budidaya Air Payau, Pusat Penelitian dan Pengembangan Perikanan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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THE EFFECT OF PHYTOECDYSTEROID OF Cycas revolua, Portulaca oleracea, AND Morus sp. ON MOLTING PERIOD, GROWTH AND SURVIVAL RATE OF TIGER SHRIMP, Penaeus monodon Rosmiati Rosmiati; Samuel Lante; Emma Suryati
Indonesian Aquaculture Journal Vol 11, No 2 (2016): (December, 2016)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (465.612 KB) | DOI: 10.15578/iaj.11.2.2016.69-74

Abstract

The problem which has still been faced in the Artificial insemination (AI) is the slow of shrimp to molt. Ecdysteroid hormone has been reported to stimulate molting of tiger shrimp. This study aims to isolate ecdysteroid hormone from Cycas revoluta, Portulaca oleracea and Morus sp. and evaluate its effect on molting period, growth and survival rate of tiger shrimp. Isolation of ecdysteroid from the leaves of three species was carried out by maceration and solvent partition method. Purification of ecdysteroid used repeated column chromatography and preparative thin layer chromatography (TLC). Evaluation of the isolated phytoecdysteroid hormone effect on molting period, growth and survival rate of shrimp was done by injecting of 100 µL phytoecdyasterod (27.5 µg/shrimp) at the first somite of ventral abdomen. As the comparison, the commercial ecdysteroid (positive control) and sterile saline solution (negative control) were also injected at the concentration of 8.6 µg/shrimp and 0 µg/shrimp, respectively. Finding showed that the highest percentage of phytoecdysteroid was obtained in Portulaca oleracea, followed by Morus sp. and Cycas revoluta with the ecdysteroid content of 0.43%, 0.22%, and 0.09%, respectively. Pytoecdysteroid isolated from the three plants was able to shorten molting period of shrimp into 4, 4, 2, and 5 days earlier for Portulaca oleracea, Morus sp., Cycas revoluta, and positive control, respectively, compared to the negative control. The highest survival rate and growth were obtained at the treatment of Portulaca oleracea, followed by Morus sp. and Cycas revoluta with the survival rate, length and weight increase of 86%, 75%, and 25%, 4.42%, 2.26% and 2.16%, and 15.90%, 10.55%, and 8.73%, respectively. 
APLIKASI INSEMINASI BUATAN PADA UDANG WINDU, Penaeus monodon ALAM MENGGUNAKAN SUMBER DAN JUMLAH SPERMATOFOR YANG BERBEDA Samuel Lante; Asda Laining
Jurnal Riset Akuakultur Vol 11, No 3 (2016): (September 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (356.153 KB) | DOI: 10.15578/jra.11.3.2016.271-280

Abstract

Salah satu kendala utama dalam domestikasi udang windu adalah rendahnya tingkat perkawinan secara alami dalam wadah budidaya. Hal yang sama terjadi pada udang windu alam yang digunakan di unit pembenihan. Salah satu upaya untuk mendapatkan telur fertil adalah melalui inseminasi buatan (IB). Inseminasi buatan merupakan teknik mentransfer spermatofor dari induk jantan dengan cara memasukkannya ke dalam telikum udang betina. Penelitian ini bertujuan untuk mengetahui performa reproduksi udang windu betina alam pasca-inseminasi menggunakan sumber dan jumlah spermatofor induk jantan alam yang berbeda. Penelitian dilakukan dua tahap yaitu 1) IB menggunakan spermatofor induk jantan dari perairan Sulawesi Selatan (SS) dan spermatofor induk jantan dari Aceh (SA) dan 2) IB menggunakan jumlah spermatofor berbeda yaitu satu spermatofor (S-1) dan dua spermatofor (S-2) pada udang windu betina alam. Inseminasi spermatofor dilakukan pada induk udang windu betina setelah dua hari moulting. Hasil yang diperoleh pada IB tahap pertama menunjukkan bahwa daya tetas telur udang windu betina alam lokal tidak dipengaruhi oleh sumber (lokasi) asal udang jantan, di mana daya tetas telur relatif sama pada kedua perlakuan, yaitu 61,6% pada SS dan 61,7% pada SA. IB pada tahap kedua menunjukkan bahwa daya tetas telur fertil yang diperoleh pada S-2 sebesar 40,5%; lebih rendah dari S-1 sebesar 44%.One of the main constraints in the domestication of black tiger shrimp is very low natural mating in the tank. Similar condition have been happened in commercial hatcheries. An effort to improve the eggs fertility is through artificial insemination (AI). This study aimed to know reproductive performance of wild black tiger shrimp after insemination with different sources and numbers of spermatophore. This study consisted of two trials.The first one was AI using spermatophores of wild male obtained from two different locations, namely from South Sulawesi (SS) and Aceh (SA). The second trial was AI using different numbers of spermatophore namely one spermatophore (S-1) and two spermatophores (S-2). AI was applied to the females at two days post-moulting. The results of the first trial showed that the hatching rate (HR) was not affected by the source of the male which was 61.6% for SS and 61.7% for SA. The second trial indicated that female inseminated S-2 had lower HR than S-1 (40.5% vs 44%).
Co-Authors Asda Laining Emma Suryati Rosmiati Rosmiati