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All Journal Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur

Samuel Lante

Balai Riset Perikanan Budidaya Air Payau dan Penyuluhan Perikanan, Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Author-ID : 3672959

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Articles

EFFECT OF VARIOUS CRYOPROTECTANTS ON PRESERVATION OF BLACK TIGER Penaeus monodon SHRIMP SPERMATOZOA Rosmiati Rosmiati; Ike Trismawanti; Samuel Lante
Indonesian Aquaculture Journal Vol 15, No 2 (2020): (December, 2020)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.15.2.2020.77-83

The development of cryopreservation technique on tiger shrimp Penaeus monodon broodstock spermatophore has been carried out to support the artificial insemination. This study aims to determine the effect of three cyoprotectants (methanol, dimetylsulphoxide (DMSO), and glycerol) for long term storage of tiger shrimp Penaeus monodon spermatozoa. Spermatophores were collected from the wild broodstocks through electrical shock. Spermatozoa were obtained by homogenizing the spermatophores using a Radnoti micro homogenizer in Ca-free saline solution containing one of three cryoprotectans (methanol, dimetylsulphoxide, and glycerol) separately at the concentration of 5%. One mL of each cryoprotectant containing spermatozoa with the density of 1.02 x 106 cell/mL was transferred into a cryovial and cryopreserved at room temperature, -20°C and -196°C for 5, 10, and 30 days. The apparent sperm viability (ASV) of cryopreserved spermatozoa was monitored after treated. Thawing of cryopreserved spermatozoa was carried out in a 30°C wáter bath for two minutes. The result showed that the best apparent sperm viability was obtained at the using of glycerol at -196°C in liquid nitrogen, even after the thirty days of cryopreservation time period with the ASV of 0.82 x 106 cells/mL (80.39%). Meanwhile two other cryoprotectans displayed the ASV of 0.54 x 106 cells/mL (56.86%), and 0.23 x 106 cells/mL (22.55%). for DMSO and methanol, respectively. In turn, the control showed the lowest ASV with the ASV of 0.01 x 106 cells/mL (1.27%). The ASV showed by this glycerol exhibited asignificant difference (P<0.05) to that of methanol, DMSO, and control.

PERFORMANSI PERTUMBUHAN DAN REPRODUKSI UDANG WINDU, Penaeus monodon YANG DIBERI PAKAN DENGAN PENAMBAHAN VITAMIN C DAN E Usman Usman; Kamaruddin Kamaruddin; Asda Laining; Samuel Lante; Bunga Rante Tampangallo
Jurnal Riset Akuakultur Vol 14, No 4 (2019): (Desember, 2019)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Penelitian ini bertujuan mengevaluasi pengaruh penambahan vitamin C dan E dalam pakan terhadap performansi pertumbuhan dan reproduksi udang windu fase prematurasi. Dua pakan uji dengan perlakuan: penambahan vitamin C (mengandung ascorbic acid 406,1 mg/kg) dan vitamin E (mengandung 286,3 mg/kg) (PS), dan tanpa penambahan vitamin C dan E (PK). Hewan uji yang digunakan adalah udang windu fase prematurasi hasil budidaya berbobot awal 43,1±5,1 g untuk betina dan 41,9±4,4 g untuk jantan, dipelihara dalam 2 petak tambak beton berukuran 1000 m2 dengan kepadatan awal 100 ekor/tambak dan rasio udang jantan : betina adalah 4 : 6. Udang tersebut diberi pakan uji sebanyak 3% dari biomassa per hari selama 3 bulan di tambak. Selanjutnya udang dipindahkan ke bak pematangan gonad untuk mengevaluasi kinerja reproduksinya. Hasil penelitian menunjukan bahwa,laju pertumbuhan spesifik, total hemosit, jumlah sel sperma, gonada somatic indeks, dan hepatosomatic indeks cenderung lebih tinggi pada udang yang diberi pakan uji PS dibandingkan yang diberi pakan PK. Udang yang diberi pakan uji PS juga memiliki fekunditas, diameter telur, daya tetas telur, dan produksi nauplii yang cenderung lebih tinggi, meskipun secara statistik tidak berbeda nyata (P>0,05) di antara kedua perlakuan. Hasil penelitian ini menunjukkan penambahan vitamin C dan E dalam pakan cenderung meningkatkan performansi pertumbuhan dan reproduksi udang windu. This experiment was aimed to evaluate the effects of feed supplemented with vitamin C and E on the growth and reproductive performances of prematurated tiger shrimp. Two test diets were formulated as the treatments: diet with the addition of vitamin C (contain ascorbic acid of 406.1 mg/kg diet) and vitamin E (contain 286.3 mg/kg diet) (PS), and a diet without the addition of vitamin C and E (PK). The test shrimp were prematurated tiger shrimp with an average initial weight of 43.1 ± 5.1 g/ind. for females and 41.9 ± 4.4 g/ind. for males. The tiger shrimp were cultured in two of 1,000 m2 concrete ponds with a density of 100 ind./pond, and the ratio of male : female was 4 : 6. The shrimp were fed with the experimental diets at a rate of 3% of body weight/day for three months. The shrimp were then transferred to concrete tanks for reproductive evaluation. The results showed that the specific growth rates, total hemocytes, number of sperm cells, gonado-somatic index, and hepatosomatic index tended to be higher in the shrimp fed with the PS test diet compared to the shrimp fed with the PK test diet. The shrimp fed with the PS test diet had higher egg fecundity, egg diameter, hatching rate, and nauplii production compared to the shrimp fed with the PK test diet. However, the study found no significant difference (P>0.05) between the two treatments. The addition of vitamins C and E in the diet could improve the growth and reproductive performance of tiger shrimp.

PENGARUH APLIKASI dsRNA VP-15 IN VITRO DAN IN VIVO TERHADAP SINTASAN DAN RESPONS IMUN UDANG WINDU Penaeus monodon Andi Parenrengi; Andi Tenriulo; Sri Redjeki Hesti Mulyaningrum; Samuel Lante; Agus Nawang
Jurnal Riset Akuakultur Vol 14, No 4 (2019): (Desember, 2019)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Teknologi RNA interference (RNAi) merupakan salah satu pendekatan yang digunakan untuk meningkatkan resistensi udang windu terhadap infeksi patogen termasuk WSSV. Pengembangan teknologi RNAi melalui aplikasi untai ganda RNA (dsRNA) yang berasal dari gen pengkode viral protein (VP) dari WSSV telah mulai dikembangkan pada udang. Penelitian ini bertujuan untuk mengkaji sintasan dan respons imun udang windu yang diberi VP-15 pasca uji tantang dengan WSSV. Udang windu (panjang 15,21 ± 1,19 cm dan bobot 32,5 ± 1,83 g) diinjeksi dengan 0,2 µg/ekor dsRNA in vitro (A), dsRNA in vivo (B), dan larutan garam/kontrol (C). Setelah tiga hari vaksinasi, udang windu ditantang dengan WSSV dengan dosis 50 µL/ekor. Pengamatan sintasan dilakukan setiap hari, sedangkan respons imun (THC dan aktivitas proPO) dilakukan pada awal dan hari ke-1, ke-3, dan ke-5 pasca uji tantang, serta analisis ekspresi gen antivirus dan histopatologi hepatopankreas dilakukan pada akhir penelitian. Hasil penelitian menunjukkan bahwa aplikasi dsRNA berpengaruh nyata (P<0,05) terhadap sintasan, THC, dan proPO. Sintasan udang windu yang diberi dsRNA VP-15 in vitro dan in vivo memberikan sintasan yang lebih tinggi 75% dibandingkan dengan kontrol. Nilai proPO tertinggi didapatkan pada dsRNA in vivo (0,138); kemudian dsRNA in vitro (0,093); dan terendah kontrol (0,061); sedangkan THC tertinggi (5.704 x 104 sel/mL) pada dsRNA in vivo, kemudian dsRNA in vitro (3.516 x 104 sel/mL) dan terendah pada perlakuan kontrol (3.322 x 104 sel/mL). Ekspresi gen antivirus semakin meningkat dengan semakin lamanya udang windu terpapar dengan WSSV. Jaringan hepatopankreas udang windu pada perlakuan kontrol (tanpa dsRNA) menunjukkan adanya kerusakan sel akibat infeksi virus.RNA interference (RNAi) technology is one of the approaches used to improve tiger shrimp Penaeus monodon resistance against WSSV infection. The development of RNAi technology through double-stranded RNA (dsRNA) isolated from gene encoding viral protein (VP) of WSSV has been applied to shrimp. This study was aimed to assess the survival rate and immune response of injected-VP-15 WSSV tiger shrimp after a challenge with WSSV. The tiger shrimp (15.21 ± 1.19 cm in length and 32.5 ± 1.83 g in weight) were injected with 0.02 µg/shrimp of in vitro dsRNA (A), in vivo dsRNA (b) and saline solution (C). After three days of vaccination, the tiger shrimp were challenged with WSSV using a dosage of 50 µL/shrimp. The survival rate was observed daily. Analyses of immune responses (hemocyte total and PO activity) were performed in several stages: before the challenge test and day-1, day-3, and day-5 post-challenge test. The expression of the antivirus gene and hepatopancreas histophatology were was observed at the end of the experiment. The results showed that the application of dsRNA significantly influenced the shrimp survival rate, THC, and proPO. Tiger shrimp injected with dsRNA VP-15 of in vitro and in vivo exhibited a higher 75% survival rate than the control (P<0.05). The highest proPO activity (0.138) was obtained at dsRNA in vivo, followed by dsRNA in vitro (0.093) and the lowest (0.061) in the control. The highest THC (5,704 x 104 cell/mL) was in vivo dsRNA, then in vitro dsRNA (3,516 x 104 cell/mL), and the lowest in the control (3,322 x 104 cell/mL). The longer the exposure with WSSV, the higher the antivirus gene expression. Histopathology analysis showed some damages to the hepatopancreas cells in the control shrimp (without dsRNA) caused by the virus infection.

PENGARUH SUMBER SPERMATOFORA PADA INSEMINASI BUATAN INDUK BETINA UDANG WINDU TURUNAN PERTAMA (F-1) TERHADAP PEMIJAHAN, KUALITAS TELUR, DAN LARVA TURUNANNYA (F-2) Samuel Lante; Usman Usman
Media Akuakultur Vol 14, No 2 (2019): (Desember, 2019)
Publisher : Pusat Riset Perikanan

Penelitian ini bertujuan mengevaluasi pemijahan udang windu betina F-1 dan mutu telur, serta larva turunannya (F-2) dengan inseminasi buatan menggunakan sumber spermatofora udang jantan yang berbeda. Perlakuan yang dicobakan adalah induk udang windu betina F-1 diinseminasi menggunakan spermatofora dari sumber induk jantan berbeda yaitu: spermatofora induk jantan F-1 hasil budidaya (S-1), dan spermatofora induk jantan alam (S-A). Data pemijahan induk betina, kualitas telur, dan profil asam amino pada daging dan hepatopankreas jantan, serta morfologi larva dianalisis secara deskriptif, sedangkan data uji vitalitas larva dianalisis uji t. Hasil penelitian menunjukkan bahwa induk udang windu betina F-1 yang diinseminasi dengan perlakuan S-1 memiliki tingkat pemijahan 67%; fekunditas 179.257 butir/induk; total produksi telur 1.434.053 butir; tingkat pembuahan telur 86,2%; daya tetas telur 59,8%; dan total produksi nauplii 738.439 ekor yang lebih rendah dibandingkan pada induk udang F-1 yang diinseminasi perlakuan S-A yang memiliki tingkat pemijahan 75%; fekunditas 215.489 butir/induk; total produksi telur 1.939.399 butir; tingkat pembuahan telur 88,9%; daya tetas telur 62,7%; dan total produksi nauplii 1.081.140 ekor. Sementara diameter telur (248-255 µm) dan mutu larva relatif sama di antara kedua perlakuan. Profil asam amino hepatopankreas dan daging pada induk udang jantan alam lebih tinggi dibandingkan pada induk udang jantan F-1. Penggunaaan spematofora jantan alam masih lebih baik daripada jantan budidaya pada inseminasi induk betina F-1 udang windu.This study was aimed at evaluating the spawning rate of F-1 female tiger shrimp and the quality of their egg and larvae derivatives (F-2) by artificial insemination using different sources of male shrimp spermatophore. The treatments consisted of broodstock of F-1 female tiger shrimp inseminated with different male spermatophores, namely: cultivated F-1 male spermatophore (S-1), and wild male spermatophore (S-A). Data on spawning performance of F-1 female tiger shrimp and amino acid profile in the hepatopancreas and muscle of male tiger shrimp and larval morphology were analyzed descriptively.The vitality of larvae was analyzed using t-test. The results showed that the broodstock of F-1 female tiger shrimp inseminated with S-1 treatment had spawning rate of 67%; fecundity of 179,257 egg; total egg production of 1,434,053 eggs; egg fertilization rate of 86.2%; hatching rate of 59.8%; and total nauplii production of 738,439 ind. Broodstock of F-1 female inseminated with S-A treatment had higher values for spawning rate of 75%, fecundity of 215,489 egg; total egg production of 1,939,399 egg; egg fertilization rate of 88.9%; hatching rate of 62.7%; and total nauplii production of 1,081,140 ind. The produced egg diameter (248-255 ¼m) and larva quality were relatively same between the two treatments. Amino acid profiles in the hepatopancreas and muscle were higher in the wild male broodstock compared to the cultivated (F-1) male broodstock. In conclusion, wild male spermatophore is generally better than the cultivated F-1 male spermatophore for artificial insemination of female broodstock F-1.

Co-Authors Agus Nawang Andi Parenrengi Andi Tenriulo Asda Laining Bunga Rante Tampangallo Ike Trismawanti Kamaruddin Kamaruddin Rosmiati Rosmiati Sri Redjeki Hesti Mulyaningrum Usman Usman

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