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DARMONO DARMONO
UNIVERSITAS PANCASILA
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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Pengaruh Ekstrak Lengkuas Putih [Alpinia galanga (L.) Willd] terhadap Infeksi Trichophyton mentagrophytes pada Kelinci DJAENUDIN GHOLIB; DARMONO DARMONO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 6 No 2 (2008): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Lengkuas putih [Alpinia galanga (L.) Willd] has long been known to be used in treatment of dermatophytosis, a skin disease caused by dermatophytes fungi such as Trichophyton mentagrophytes. The fungi infects hairs, skins, and nails that contain keratin. The study was conducted in order to measure the effect of ethanol extract of Alpinia galanga in inhibiting Trichophyton mentagrohytes colonies both through in vitro test and in vivo test using infected rabbits . The results showed that minimal inhibition concentration (MIC) of Ahoinia galanga extract Was 1.5%. The extract was administered in cream preparation on in vivo test with minimal concentration (1.5%). Three group of rabbits were treated with the extract cream preparation (Group I), 2% with ketokonazol cream (Group II, positive control), and With none (Group III, negative control), respectively. The results showed that the infected rabbits treated with either cream containing the extract or ketokonazol were better recovered from infection than the rabbits in negative control group.
Sensitivitas Metode Bioautografi Kontak dan Agar Overlay dalam Penentuan Senyawa Antikapang ENI KUSUMANINGTYAS; ESTIE ASTUTI; DARMONO DARMONO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 6 No 2 (2008): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Bioautography method is a laboratory technique to detect substances inhibiting the growth of tested microorganisms. The sensitivity of contact and agar overlay bioautography Was compared to define a recommended method for detecting antifungal compound. In contact bioautography, n-hexane extract of Alpinia galanga was eluted with dichloromethane and toluene on a thin layer chromatography plate (silica gel GF254). The separated compounds were diffused from chromatogram spots to a Thrichophyton menzagrophytes inoculated Sabouraud Dextrose Agar plate. The plate was incubated at 37°C for 3 days. Inhibition zone was observed and the hRf value was defined. Agar overlay bioautography Was performed by covering chromatogram spots With a molten Sabouraud Dextrose Agar medium seeded by Trichophyton mentagrophytes. After solidification, the chromatogram plate was incubated at 37°C overnight and then was stained with Methyl Thiazole Tetrazolium. The inhibition growth bands were visualized and hRfs value were recorded. A contact bioautography produces oneband while agar overlay bioautography produces four bands. Agar overlay bioautography was therefore more sensitive than contact bioautography.
Co-Authors Djaenudin Gholib Eni Kusumaningtyas ESTIE ASTUTI