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Red Rosella Calyx Extract Prevent the Increase of Malondialdehyde of Wistar Rats Exposed to Cigarette Smoke ARYA ULILALBAB; BAMBANG WIRJATMADI; MERRYANA ADRIANI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 13 No 2 (2015): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Saat ini, jumlah perokok di Indonesia masih tinggi. Asap rokok mengandung radikalbebas. Penelitian ini bertujuan untuk menganalisis dampak pemberian ekstrak rosella merah terhadappencegahan kenaikan malondialdehid (MDA) serum tikus wistar yang dipapar asap rokok. Total sampel20 tikus jantan dan dibagi menjadi empat kelompok: kontrol negatif, kontrol positif, perlakuan ekstrakkelopak rosella merah dosis 270 mg/bb dan ekstrak kelopak rosella merah dosis 540 mg/bb. Kontrolnegatif hanya diberi pakan standar. Kontrol positif diberi pakan standar + dipapar 2 rokok per hari.Kelompok perlakuan diberi pakan standar + ekstrak kelopak rosella merah di pagi hari dan setelah itudipapar 2 rokok. Penelitian ini dilakukan selama 28 hari. Di akhir penelitian, serum darah dianalisisuntuk menentukan nilai MDA. Hasil penelitian uji MDA serum dianalisis dengan One Way ANOVA dandiikuti oleh uji Tukey HSD pada level 5%. Pemberian ekstrak kelopak rosella merah dosis 270 mg/kgbb dan ekstrak kelopak rosella merah dosis 540 mg/bb dapat mencegah peningkatan MDA serum secarasignifikan pada tikus wistar yang dipapar asap rokok.
Hubungan antara Aktivitas Fisik dan Aktivitas Sedentari dengan Status Gizi Lebih pada Anak Sekolah Dasar Erlina Nurlaili Rahma; Bambang Wirjatmadi
Amerta Nutrition Vol. 4 No. 1 (2020): AMERTA NUTRITION
Publisher : Universitas Airlangga, Kampus C, Mulyorejo, Surabaya-60115, East Java, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.429 KB) | DOI: 10.20473/amnt.v4i1.2020.79-84

Abstract

Background: Overnutrition status can be influenced by multifactor such as behaviors are eating habits, physical activity, sedentary activity, and genetic. Objectives: Analyze the relationship between of physical activity and sedentary activity with overnutrition status of elementary students.Methods: The study using a case control design, with respondents were 22 students in overnutrition status group and 22 students in normalnutrition status group.  Simple random sampling was used this study. Data were collected throught direct interview using Physical Activity Questionnare-Children (PAQ-C) to record respondent’s physical activity, and Adolescent Sedentari Activity Questionnaire (ASAQ) to record respondent’s sedentary activity. Analysis data using Chi-Square test for physical activity and Spearman test for physical activity. Results: The result showed that there was a relationship between physical activity with overnutrition status (p=0.016) and an OR=0.218 with CI 95% (0.061 – 0.775) which mean that student who did physical activity with good category at risk 0.218 times less to be overnutition status. As for sedentary activity (p=0.026) with OR=5.5 and CI 95% (1.145–17.679), which mean students who did sedentary activity more than 5 hour at risk 4.5 times greater than to be overnutrition status compared with students who did physical activity less than 2 hour. Conclusion: The low of physical activity and high of sedentary activityin elementary students were related with overnutrition status.  Student with overnutrition status must be increase physical activity and reduced sedentary activity.  ABSTRAK Latar Belakang: Status Gizi Lebih disebabkan oleh multifaktor yaitu faktor perilaku seperti kebiasaan makan, aktivitas fisik, aktivitas sedentari, dan faktor genetik.Tujuan: Menganalisis hubungan antara aktivitas fisik serta aktivitas sedentari dengan status gizi lebih pada anak usia sekolah dasar.Metode: Penelitian ini menggunakan desain case control, dengan sampel 22 anak kelompok status gizi lebih dan 22 anak kelompok status gizi normal. Simple random sampling merupakan metode yang digunakan dalam pengambilan sampel. Pengumpulan data menggunakan metode wawancara langsung dengan bantuan kuesioner PAQ-C (Physical Activity Questionnare-Children) untuk mencatat aktivitas fisik, dan kuesioner ASAQ (Adolescent Sedentari Activity Questionnaire) untuk mencatat aktivitas sedentari. Data dianalisis dengan uji statistik Chi-Square untuk aktivitas fisik dan Spearman untuk aktivitas sedentari.Hasil: Hasil penelitian menunjukkan bahwa terdapat hubungan yang signifikan antara aktivitas fisik dengan status gizi lebih (p 0,016) didapatkan nilai OR= 0,218 dengan CI 95% (0,061 – 0,775) dapat diartikan bahwa siswa yang melakukan aktivitas fisik dengan kategori baik beresiko 0,218 kali lebih kecil mengalami status gizi lebih. Sedangkan, untuk aktivitas sedentari (p= 0,026) dengan nilai OR = 4,5 dan CI 95% (1,145 – 17, 679) artinya siswa yang melakukan aktivitas sedentari > 5 jam maka memiliki kecenderungan 4,5 kali lebih besar untuk mengalami status gizi lebih dibandingkan dengan siswa yang melakukan aktivitas sedentari < 2 jam.Kesimpulan: Rendahnya aktivitas fisik dan tingginya aktivitas sedentari pada anak sekolah dasar berhubungan dengan masalah status gizi lebih. Siswa dengan status gizi lebih sebaiknya melakukan aktivitas fisik lebih banyak lagi, dan mengurangi kegiatan yang kurang gerak.
Selenium Linked to Increased Antioxidant Levels and Decreased Free Radicals in Lung Tissue of Wistar Rats Exposed to E-Cigarette Smoke Bambang Wirjatmadi
Journal of Global Pharma Technology Volume 12 Issue 09 (2020) Sept. 2020
Publisher : Journal of Global Pharma Technology

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Abstract

Objective: This study aimed to find out whether selenium could increase levels of antioxidants and decrease free radicals in lung tissue of Wistar rats after exposure to e-cigarette smoke. Methods: This study used an experimental method with control group design. Male Wistar rats with criteria aged 2–3 months, weighing 200-250 grams were used as animal models in this study to assess cell damage through the expression of Superoxide Dismutase, Glutathione Peroxidase and Malondialdehyde in the lung tissue using immunohistochemical staining. E-cigarette smoke containing 6 mg of nicotine was given to each group of animal models with differences in the amount and duration of time. The adaptation process in experimental animals was carried out for approximately 5 days. Results and Conclusion: Selenium has been shown to increase the expression of antioxidant superoxide dismutase and glutathione peroxidase, and decrease the levels of malondialdehyde in cells following oral administration of selenium in Wistar rats (p <0.05). In addition, selenium also reduced the amount of free radical in the cells; when it was given before exposed to e-cigarette smoke. It can be concluded that oral selenium can increase antioxidants levels and decrease free radicals in lung tissue cells due to exposure to e-cigarette.
PENGARUH MARGARIN DAN MINYAK KELAPA SAWIT DENGAN PEMANASAN BERULANG TERHADAP KADAR INSULIN DARAH PUASA PADA TIKUS WISTAR Retno Larasati; Bambang Wirjatmadi; Merriyana Adriani
STRADA : Jurnal Ilmiah Kesehatan Vol. 5 No. 2 (2016): November
Publisher : Universitas STRADA Indonesia

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Abstract

Margarine and coconut oil is a food that is commonly consumed by the population of Indonesia, both these foods are a source of trans fatty acids that can induce insulin resistance that eventually causes hyperinsulinemia. This study aims to analyze the differences in fasting blood insulin levels among both the control group and the treatment that is given Trans Fatty Acid (ma rgarine and palm oil with repeated heating). Method used purely experimental approach Post Test Only Control Group Design. Samples used 25 male Wistar strain rats were divided into 5 groups. The collection of data obtained from the results of laboratory examinations of fasting blood insulin. Research results from the test with one way Anova with a confidence level of 95%. The result showed fasting insulin levels between the two groups did not differ significantly. It was concluded that margarine and palm oi l with repeated heating does not cause hyperinsulinemia, but the group given both these foods showed a tendency to increased levels of fasting blood insulin