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Sumaryanto Sumaryanto
Universitas Pancasila
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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APLIKASI REAKTOR SISTEM SINAMBUNG DAN TEKNOLOGI MEMBRAN PADA PRODUKSI ENZIM A-AMILASE OLEH Bacillus subtilis Mahyudin AR; Sumaryanto Sumaryanto
JURNAL ILMU KEFARMASIAN INDONESIA Vol 1 No 2 (2003): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

a-Amylase enzyme (endoamylase) is produced for hydrolysis of 1,4-glikosidic binding from starch to maltose in food and non food industries. Due to increasing production capacity, continuous of fermentation process and maintaining the enzyme activity can be achieved with CSTR (Continuous Stirred Tank Reactor), an alternative for batch process replacement. 3,51 Reactor combined with microfiltration membrane under specific conditions such as : pH, molasses-urea medium, temperature, and agitation are adjusted to 8,37° C, and 250 rpm. The result obtained showed that in 1 vv m aeration Bacillus subtilis ATCC 6633 reached an enzyme specific activity of 93 Unit/mg protein during 20 hours fermentation. By continuous fermentation using a medium containing 3 % molasses - 0,6 % urea, these bacteria excrete an enzyme with a specific activity of 60 U/mg protein during 22 hours fermentation. It was shown that a production during 96 hours gave an enzyme production with a specific activity of 90 U/mg protein.
KINETIKA PERTUMBUHAN BEBERAPA MIKROBA PENGHASIL A - AMILASE MENGGUNAKAN MOLASE SEBAGAI SUMBER KARBON Trismilah Trismilah; Sumaryanto Sumaryanto
JURNAL ILMU KEFARMASIAN INDONESIA Vol 1 No 2 (2003): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Molasse as a waste product in sugar industry can be used as alternative carbon source in fermentation, which influences the growth of microbes and production process. In this experiment 5% molasses was used as a medium for producing a - amylase using Bacillus subtilis ATCC 6633, Streptomyces fradiae, and Aspergillus niger. The experiment was done in an Erlenmeyer of 125 ml in a shaker incubator with 250 rpm agitation. The temperature was adjusted to 37°C and the initial pH for Bacillus subtilis ATCC 6633: waste pH=8; Streptomyces fradiae: pH=7, and Aspergillus niger : pH=5. The result showed, that the highest growth and enzyme activity were accomplished by Bacillus subtilis ATCC, while specific growth rate (u max)= 0,1501 in 24 hours attained.
Perkembangan Bioteknologi Masa Kini Sumaryanto Sumaryanto
JURNAL ILMU KEFARMASIAN INDONESIA Vol 1 No 1 (2003): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

The modern products of biotechnology are, vaccine, interferon, interleukin, monoclonal antibody, new rice varieties, antibiotics, biological pesticides such as bacteria Bacillus thuringiensis which are active against specific insect species and their application through transgenic plants. There is a great difference in the products if compared with those of the conventional biotechnology, such as soy sauce, fish sauce, yoghurt, ethanol and tempe. Nowadays HIV/AIDS, pneumonia virus SARS (severe acute respiratory syndrome), genetic disorder, cloning, biological weapon such as anthrax from Bacillus anthraxis, are the kinds of several diseases, discoveries and conditions which look for development and clarification through biotechnology. It was shown in this paper some new biotechnology products, technology scope closely related to biotechnology and also the comparation of national priorities and strategy for developing of biotechnology in developed and developing countries.
Co-Authors Mahyudin AR Trismilah Trismilah