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Pengkulturan Sampel Susu Fermentasi yang Mengandung Probiotik dan Identilikasinya dengan PCR AMARILA MALIK; INDRIANI INDRIANI; LILIANA SARAGIH; MAKSUM RADJI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 9 No 1 (2011): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1605.494 KB)

Abstract

Lactic Acid Bacteria (LAB) has long been used in fermented milk because its stability, unique taste and texture and also for its health benents that are provided by the probiotic. There are many fermented dairy products in the market nowadays that declared containing living culture or as probiotics. The aim of this study was to identify LAB from fermented milk product by Visual inspection on plate culture for their morphology, as well by molecular technique performing PCR and DNA sequencing methods. Morphology and growth incubation time of each isolate was observed. A pair of primer that is highly speciiic for LAB targeting the gene encoding for 168 rRNA, which represents sequences of region 677 to 693 of E. coli numbering has been used for PCR identiiication in this study. A total of nineteen isolates were obtained from 10 fermented milk samples that were grown on de Mann Rogosa Sharpe Agar (MRSA) media. Nine out of 19 isolates which represent each of the samples were selected. Genomic DNA extracted from those 9 isolates were used as template for PCR. The results showed that 8 amplicons were positively identitied as LAB, but only three identities indicated similar species as declared, while 5 others did not.
EVALUASI PENERAPAN AUTOMATIC EXCHANGE OF INFORMATION (STUDI BANDING INDONESIA DAN JEPANG) indriani indriani; M. A. Vishu Juwono; Henderi Gunadi
Accounting Global Journal Vol 5, No 2 (2021): Accounting Global Journal
Publisher : Universitas Muria Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24176/agj.v5i2.6421

Abstract

Penerapan Automatic Exhange of Information (AEoI) telah memasuki tahun ketiga,  Indonesia dan Jepang telah menjadi salah negara yang mengimplementasikan AEoI secara bersamaan dalam perjanjian internasional. Penelitian ini dilakukan untuk menjelaskan, menganalisis dan mengetahui terkait evaluasi kebijakan AEoI yang telah dilakukan di Indonesia dan Jepang dalam proses pemeriksaan untuk penggalian potensi penerimaan pajak, penelitian ini dilakukan dengan menggunakan pendekatan kualitatif dan teknik pengumpulan data melalui wawancara mendalam dan studi literatur. Hasil penelitian ini menunjukkan bahwa implementasi kebijakan AEoI di Indonesia sudah baik walaupun masih terdapat kendala terkait ketidaklengkapan data hasil pertukaran informasi secara otomatis yang didapatkan melalui lembaga keuangan sedangkan di Jepang sudah berhasil hal ini didukung oleh kepatuhan pajak yang tinggi oleh wajib pajak di Jepang, penggunaan single identity number, sistem yang telah terbangun, dan standar yang telah ditetapkan.