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Bambang Sukilarso Sakiman
Faculty of Medicine, Sebelas Maret University
Biochemistry, Genetics & Molecular Biology

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Pengaruh Ekstrak Buah Jambu Biji Merah terhadap Kerusakan Struktur Histologis Hepar Mencit yang Diinduksi Asetaminofen Wardana, Vasa Adi Wisnu; ., Suyatmi; Sakiman, Bambang Sukilarso
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Antioxidants in guava fruit are vitamin C, flavanoid, and lycopene which are estimated having hepato-protective function. The objectives of this research are to know the effect of guava fruit extract and in various doses on mice liver damage induced by acetaminophen. Methods: Samples of 28 male mice, Swiss webster type, 2-3 months old age and + 20gr of each weight, were divided into 4 groups of 7 mice. Guava fruit extract was given for 14 days in a row, while acetaminophen in toxic dose was given on the 12th, 13th, and 14th day. The score of hepatocyte damage was determined by pyknosis, karyorhexis, and karyolysis. The data was analized by One-Way ANOVA test (α= 0,05) and continued by Post Hoc Multiple Comparisons using Least Significant Differences(LSD) test(α= 0,05). Results:The group with the highest average damage of hepatocyte is KP1(84,23±3,207), and the groups with decrease average damage of hepatocyte are KP2 (48,76±2,964) and KP3(35,23±2,755). The results of One-Way ANOVA test showed a significant difference in all group (p<0.001). The statistical data result showed a significant difference of hepatic damage between K-P1, K-KP2, K-KP3, KP1-KP2, KP1-KP3, and KP2-KP3. Conclusion: Guava fruit extract can decrease the histological damage of the hepatocyte of mice induced by acetaminophen. The higher dose of guava fruit extract, the higher protection effect againts liver histological damage from acetaminophen toxic on mice. Key words: guava fruit extract, acetaminophen, histological damage, hepatocyte.
Pengaruh Ekstrak Daun Salam (Syzygium polyanthum Wight) Terhadap Waktu Kematian Ascaris suum,Goeze In Vitro Andaru P, Gagat Ragil; Sakiman, Bambang Sukilarso; Haryati, Sri
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Syzygium polyanthum Wight leaves contain tannin that has been known to have anthelmintic effect. This study aimed to determine the effect of Syzygium polyanthum Wight leaves extract toward death time of Ascaris suum, Goeze In Vitro Methods: The study was a laboratory experimental research using the post-test only controlled group design. Subjects were adult Ascaris suum, Goeze. The sampling technique used was purposive sampling. Subjects were divided into 7 groups, each group consisted of 4 worms, replication was performed 4 times. Saline solution was used in negative control group, pirantel pamoate 5 mg/ml was used in positive control group, while the treatment group used Syzygium polyanthum Wight leaves extract concentration of 70%, 75%, 80%, 85%, and 90%. Worms were immersed in 25 mL of the test solution and incubated at 37°C. Observations were done every 1 hour and the number of dead worms was counted. Data were analyzed with regression linier and probit analysis. Results: Observations of total deaths mean time Ascaris suum, Goeze sp on negative control was for 96 hours, on the positive control was for 2 hours 30 minutes, and on the concentration of 70%, 75%, 80%, 85%, and 90% of Syzygium polyanthum Wight leaves extract, each was for 10 hours 15 minutes, 8 hours, 6 hours, 4 hours 45 minutes and 3 hours, consecutively. Linear regression test results showed that the variation of the concentration affected long worm death. Probit analysis results showed that LC50 and LT50 of Syzygium polyanthum Wight leaves extract, each was 76.228% and 3 hours 36 minutes. Conclusions:Based on this study, it could be concluded that of Syzygium polyanthum Wight leaves extract affected mortality of Ascaris suum, Goeze sp in vitro. The increasing of extract concentration was inversely proportional with the death time of worms Keywords: Syzygium polyanthum Wight leaves extract, Ascaris suum,Goeze.
Efek Antihelmintik Ekstrak Akar Ceguk (Quisqualis indica, L.) pada Ascaris suum, Goeze in Vitro Kurnia, Antonius Bagus Budi; Haryati, Sri; Sakiman, Bambang Sukilarso
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Radix-Quisqualis indica L. contains saponins and potassium quisqualata that are known having anthelmintic effect. This study aimed  to determine the effect of extract of radix-Quisqualis indica L. toward mortality of Ascaris suum, Goeze  in vitro Methods: This study was a laboratory experimental research using the post-test only controlled group design. Subjects were adult Ascaris suum, Goeze. The sampling technique used was purposive sampling. Subjects were arranged into 7 groups, each group consisted of 4 worms, replications were performed 4 times. Saline solution was used in negative control group and pyrantel pamoate 5 mg/ml was used in positive control group, while the treatment group was treated with extract of radix-Quisqualis indica, L. at concentration of 50%, 60%, 70%, 80%, and 90%. Subjects  were immersed in 25 ml of  test solution and incubated at 37°C. Observations were done every 1 hour and the numbers of dead worms were counted. Data were analyzed with linier regression and probit analysis. Results: The result of observation showed that mean time of total death of Ascaris suum, Goeze  in the negative control and in the positive control were 96.00 hours and 1.25 hours, each. While mean time of total death of Ascaris suum, Goeze in the extract of radix-Quisqualis indica, L. at concentration of 50%, 60%, 70%, 80%, 90% were 11.50 hours, 9.50 hours, 8.00 hours, 5.75 hours and 3.50 hours, consecutively. Shapiro Wilk test showed that the distribution of the data was normal (p > 0.05). Linear regression test showed that the variation of the extract concentration affected time of worm death. Probit analysis showed that LC50 and LT50 of the extract of radix-Quisqualis indica L. were 69.80% and 3.90 hours. Conclusions: The extract of radix-Quisqualis indica, L. affected mortality of Ascaris suum, Goeze  in vitro. Keywords: extract of radix Quisqualis indica, L., Ascaris suum, Goeze,
Perbedaan Daya Anthelmintik antara Ekstrak Daun Pepaya (Carica papaya, Linn.), Daun Pare (Momordica charantia, Linn.), dan Kombinasi Keduanya terhadap Cacing Ascaris suum, Goeze in Vitro Swadini, Nurul Rahmawati; Haryati, Sri; Sakiman, Bambang Sukilarso
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Backgrounds: Ascariasis is an infection disease caused by Ascaris lumbricoides, Linn. Drugs therapy for ascariasis has various side effects causing unpleasant sensation to the patient such as nausea, vomiting, diarrhea, and constipation. Infusion of papaya leaves and bitter gourd leaves contains saponin and tannin known having anthelmintic activity. This experiment aimed to find out the difference of anthelmintic capacity among papaya leaves extract, bitter gourd leaves extract and their combination to Ascaris suum, Goeze, in vitro. Methods: This experiment was quasi experiment with post test only controlled group design. Subject for this experiment was Ascaris suum, Goeze. The subject was arranged into five groups, those were negative control group (NaCl 0.9%), positive control group (Pirantel pamoat 5 mg/ml), papaya leaves extract group, bitter leaves extract group and the combination of both papaya and bitter gourd leaves extract group. The extract concentrations for the treatment were 40%, 50%, 60%, 70%, and 80% w/v. Subjects were dipped into extract for maximum monitoring time. Death time of all worms in each group was examined. Data were analyzed with linear regression and probit analysis (α = 0.05). Results: Regression analysis showed that there was a significant relation between death time and concentration of papaya leaves extract, bitter gourd leaves extract, and combination of both with significance value p = 0,000 (p < 0.05). Probit analysis showed that combination of papaya leaves and bitter gourd leaves extract was more effective than Pyrantel pamoate. Conclusions: There were differences of anthelmintic capacity among papaya leaves extract, bitter gourd leaves extract and their combination. The combination of both papaya leaves extract and bitter gourd leaves extract as anthelmintic was more effective than they were alone and also more effective than Pyrantel pamoate. Keywords: Anthelmintic capacity, papaya leaves extract, bitter gourd leaves extract, combination
Pengaruh Ekstrak Biji Kedelai Putih (Glycine max) terhadap Waktu Kematian Cacing Gelang Babi (Ascaris suum) in Vitro ., Pritami; Haryati, Sri; Sakiman, Bambang Sukilarso
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background : White Soy beans (Glycine max) contain saponin and tripsin inhibitor that have been known having anthelmintic effect. This study aimed to determine the effect of White Soy beans extract toward death time of Ascaris suum, in vitro. Methods : The study was a laboratory experimental research using post – test only controlled group design. The subjects were an adult male and female worms (Ascaris suum) that their body length were around 15 cm – 35 cm. The sampling technique used was convenience sampling. The subject was arranged into 6 groups, each group consisted of 4 worms, replication was performed 4 times. NaCl 0.9 % and pirantel pamoat 5 mg/ml was added in the negative control group and in the positive control group, in a row.While white soy beans extract of concentration of 60%, 70%, 80%, and 90% w/v were added to the treatment group. Worms were immersed in 25 ml test solution and incubated at 37°C. The subjects were observed every 10 minutes and the number of dead worms was counted. Data were analyzed with linear regression and Pearson correlation. Results : The data of observation showed that total death mean time of Ascaris suum in the negative control and positive control were 2817.5 minutes and 42.5 minutes, while in the treatment group of concentration of 60% , 70% , 80% , and 90%  w/v of white soy beans extract were 422.5 minutes, 370 minutes, 300 minutes, and 255 minutes, successively. The Pearson correlation test showed very close relationship between variation of the concentration and time of death worms (r = - 0.962). The linear regession test showed significantly (p<0.001) and the regression equation was Y = 2653.400 – 30.340 X and R square was 0.926. Conclusion : White soy beans (Glycine max) extract had effects to the mortality of Ascaris suum in vitro. The most effective white soy beans extract was 90% (w/v) although it was less effective than pirantel pamoat. Keywords : White Soy beans (Glycine max) Extract, Ascaris suum, death time. 
Pengaruh Ekstrak Wortel (Daucus carota L.) terhadap Waktu Kematian Cacing Ascaris suum, Gooze in Vitro Seno, Aryo; Haryati, Sri; Sakiman, Bambang Sukilarso
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background : Carrots contain quercetin that can be used as antihelmintik. Quercetin is a class of flavonoid glycosides. This study aimed to determine the effect of carrot’s extract (Daucus carota, L.) against the death time of Ascaris suum, Goeze in vitro. Methods :This study was a laboratory experimental research using post-test only controlled group design. Subjects were Ascaris suum, Goeze which were still in active movement. Sampling technique used in this study was convenience sampling. Subjects were arranged into 7 groups, each group consisted of 4 worms, replication was done 4 times. Pyrantel pamoate 5mg/ml and physiological saline were successively added to the test solution of positive control and negative control group, while carrot’s Extract (Daucus carota, Linn) of 70%, 75%, 80%, 85% and 90% were added to the test solution of treatment groups. Worms were soaked in 25 ml of test solution and incubated at 37ºC. Observations were done every hour until all the worms died. Data were analyzed using Pearson correlation and linear regression. Results : There was a significant correlation between the concentration variables and time of death of the worms variable (p value of Pearson correlation was 0.000 (<0.050). The higher concentration of the carrot extract was the shorter time of death of worms (Correlation coefficient of concentration variable and time of death of the worms variable was - 0.960). The linear regression equation was Y = 53.775 - 0.609X. Conclusion : The Carrot’s extract (Daucus carota, Linn) had effects on accelerating  the time of death of Ascaris suum, Gooze in vitro. Keywords : Daucus carota, Linn extract, Ascaris suum, Goeze 
Co-Authors Antonius Bagus Budi Kurnia Aryo Seno Gagat Ragil Andaru P Nurul Rahmawati Swadini Pritami . Sri Haryati Suyatmi . Vasa Adi Wisnu Wardana