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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Biotropika
Setijono Samino
Department of Biology, University of Brawijaya, Malang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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UJI TOKSISITAS AKUT BIOPESTISIDA PADA Bellamya javanica, v.d Bush 1884 DAN Lymnaea rubiginosa, Michellin 1831 Agung Willy Pramana, Ida IDewa; Samino, Setijono
Biotropika: Journal of Tropical Biology Vol 2, No 4 (2014)
Publisher : Biotropika: Journal of Tropical Biology

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ABSTRACT Acute toxicity test is one method of pre-clinical trials are used to determine the degree of toxicity of a compound within 24 Hours. Biopesticides are a natural pesticide products made ​​from plants. The purpose of this study was to determine the acute toxicity value of biopesticides against Bellamyâ javanica, v.d Bush 1884  and Lymnaea rubiginosa, Michellin 1831 with quantitative measurement using LC50 with a span of 24, 48, 72, and 96 hours. The study was conducted in a laboratory scale with a completely randomized factorial design, using a series of six concentrations and three replicates for each species. LC50 analysis using probit analysis. The results of this study indicate LC50 values ​​for B. javanica, v.d Bush 1884 at observation time 24 hours, 48 hours, 72 hours, and 96 hours is 38.418%, 18.820%, 11.817% and 6.637% and LC50 values ​​for L. rubiginosa, Michellin 1831 at observation time 24 hours, 48 hours, 72 hours, and 96 hours is 6.704%, 4.513%, 3.451%, and 1.307%. Keywords : Biopesticides, Abiotic factors, LC50,  Acute toxicity
Uji Toksisitas Bacillus thuringiensis Asal Kota Nganjuk Terhadap Larva Aedes aegypti Pratiwi, Erma Kusuma; Samino, Setijono; Gama, Zulfaidah Penata
Biotropika: Journal of Tropical Biology Vol 1, No 4 (2013)
Publisher : Biotropika: Journal of Tropical Biology

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ABSTRAK   Demam Berdarah Dengue (DBD) merupakan salah satu penyakit endemik di Indonesia dan menyebabkan banyak kematian penduduk, khususnya di Provinsi Jawa Timur. Kota Nganjuk memiliki tingkat kelimpahan larva Aedes aegypti tertinggi di Provinsi Jawa Timur pada tahun 2008-2010. Kota Nganjuk merupakan daerah fokus utama untuk penanganan penyakit endemik DBD di Indonesia. Tujuan penelitian ini untuk mendapatkan isolat Bacillus thuringiensis asal Kota Nganjuk yang paling efektif dalam membunuh larva Aedes aegypti. Sampel sedimen dan air diambil dari 10 lokasi di Kecamatan Nganjuk. Bakteri diisolasi menggunakan media selektif B. thuringiensis kemudian dilakukan karakterisasi fenotip (Profil Matching Method), seleksi isolat yang patogen dan uji toksisitas (LC50) pada larva nyamuk Aedes aegypti instar III. Persentase mortalitas larva dianalisis probit (LC50) dan ragam (ANOVA). Hasil isolasi mendapatkan dua isolat B. thuringiensis yaitu K.K1.S.K2 dan W.Swh.S.K2 dari 26 isolat bakteri yang mampu membunuh larva Aedes aegypti instar III lebih dari 50 %. Isolat  K.K1.S.K2 serta W.Swh.S.K2 dengan umur biakan 48 jam pada waktu pendedahan 72 jam efektif membunuh larva Aedes aegypti instar III secara berurutan yaitu 83,3 % dan 76,67 %. Isolat W.Swh.S.K2 hasil isolasi dari sampel sedimen memiliki toksisitas tertinggi dengan nilai LC50 48 jam sebesar 3,53x107 sel/ml.   Kata Kunci : Aedes aegypti, Bacillus thuringiensis, Demam Berdarah Dengue, Uji Toksisitas
Daya Repelensi Biopestisida Terhadap Walang Sangit (Leptocorisa oratorius, Fabricus) di Laboratorium Sihombing, Maic Audo Eybi Mayer; Samino, Setijono
Biotropika: Journal of Tropical Biology Vol 3, No 2 (2015)
Publisher : Biotropika: Journal of Tropical Biology

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Biopestisida adalah pestisida organik yang dipakai untuk mengendalikan populasi hama di bawah batas ambang ekonomi. Tujuan penelitian ini adalah untuk mendapatkan konsentrasi efektif biopestisida yang dapat mengusir walang sangit dewasa, baik pada kondisi gravid dan non gravid (kondisi campur) dan non gravid. Walang sangit dewasa diaklimatisasi selama satu minggu di laboratorium. Konsentrasi biopestisida yang digunakan adalah 0 %, 1,79 %, 3,57 %, 7,14 %, 14,29 %, dan 50 %. Uji repelensi dilakukan dengan menggunakan olfaktometer empat lengan yang dimodifikasi menjadi binary choice. Data dianalisis dengan uji T tidak berpasangan, SPSS 16 untuk Windows. Seluruh konsentrasi biopestisida efektif untuk mengusir walang sangit dewasa dalam kondisi campur (P>0,05) dengan daya repelensi berkisar antara 70-90 %. Konsentrasi efektif biopestisida untuk mengusir walang sangit dewasa dengan kondisi non gravid yaitu 14,29 % dan 50 % dengan daya repelensi berturut-turut yaitu 83,33 % dan 90,91 %. Durasi waktu yang dibutuhkan oleh walang sangit dewasa kondisi campur bervariasi untuk setiap konsentrasi efektif (50 % : 23,13 detik;   14,29 % : 27,67 detik; 7,14 % : 27,11 detik; 3,57 % : 23,50 detik;  1,79% : 45,70 detik). Durasi waktu yang dibutuhkan oleh walang sangit dengan kondisi non gravid pada konsentrasi efektif yaitu 13,91 detik dan 18,70 detik. Kata kunci: Biopestisida, Leptocorisa oratorius, Fabricus, Olfaktometer Binary Choice, Repelensi
Alteration of Coral Reef Community Structure Caused by Athropogenic Activity in Coastal Area of Giliketapang Island, Probolinggo, East Java, Indonesia Puspitasari, Lutfiatul; Samino, Setijono; Retnaningdyah, Catur
Journal of Tropical Life Science Vol 3, No 1 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

The aim of this research was to know the effect of anthropogenic activity around the coastal Giliketapang Island to community structure of coral reef. This research used belt transect method which consist of 18 transects in three location (harbour,near the forest and transition areas)at 50-80 m from the coast, with plot size of 2x2m2. Abundance of coral reefs were observed base on the coverage. There are found 11 families of coral reefs. The percentage of coral damage was decrease and the number of species was increase at the far zone from coast. This is also affect the coverage value of coral reefs. Coral coverage at the harbour and near the forest was 53. 06 % and 65. 36 % respectively, whereas in the transition area was 38. 38%. Co-domination between Porites lobata and Chyphastrea microphthalma occurred at the harbour, while Porites mayeri and Acropora spp. co-dominate in near the forest location. Poritidae family dominate in the transition area. Anthropogenic activities at transition location cause severed damage of coral reefs.
Organic Agroedu-Tourism Attractions Toward An Interesting Outdoor Environmental Education To Junior High School Students Nisa', Ayu Raisa Khairun; Samino, Setijono; Arisoesilaningsih, Endang
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

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The purpose of this study is to evaluate two organic agroedu-tourism attractions developed to 42 students of eight graders of three junior high schools in the village and one school in the city. Organic agroedu-tourism offers two tour programs namely Organic Garden Tour and Organic Farming Technology. Appreciations of the participants were determined by pre and post test after joining the organic agroedu-tourism. The data were analyzed by using descriptive statistics. This study shows that 78.6 % of the participants prefer the Organic Garden Tour and 21.4% find that the Organic Farming Technology is better. Organic agroedu-tourism can improve cognitive aspect of participants from score 1 turned into 3. Most participants had a high interest in joining the organic egroedu-tourism. Organic agroedu-tourism is also able to meet the expectations of participants that are indicated by an increase in the skills and experience of participants from score 2 to 5. Satisfaction of the participants can also be assessed from the high appreciation average before and after joining Organic agroedu-tourism, which are score 4. The appreciation of the participant is shown by their willingness to invite other people to join. Factors affecting participants satisfaction were family background, socio-economy background, personal perception and knowledge. Based on the result, organic agroedu-tourism shows strength to develop the participants understanding, knowledge and skills in integrated organic farming system, even though not all participants are interested to join all programs provided. Farmer groups need to improve the quality of organic agroedu-tourism, for example, completing the facilities and agreed on tourism attractions, enhancing farmers skills as tour guide, providing organic agroedu-tourism program relevant to the school curriculum, and promoting it to public.
Co-Authors Ayu Raisa Khairun Nisa’ Catur Retnaningdyah Endang Arisoesilaningsih Erma Kusuma Pratiwi Ida IDewa Agung Willy Pramana Maic Audo Eybi Mayer Sihombing, Maic Audo Eybi Mayer Nisa', Ayu Raisa Khairun Puspitasari, Lutfiatul Zulfaidah Penata Gama