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Agus Nuryanto

Fakultas Biologi, Universitas Jenderal Soedirman

Author-ID : 3698241

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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DIVERSITAS DAN DISTRIBUSI IKAN DI SEGARA ANAKAN CILACAP Suprastini Suprastini; Erwin Riyanto Ardli; Agus Nuryanto
Scripta Biologica Vol 1, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Fish is one of the aquatic commodities with significant economic value, and Segara Anakan areas is a habitat for valuable fishes for local fisherman. Segara Anakan is a eutrophic waters and serves as nursery and spawning ground and also provide an optimal environment for several species fish larvae to grow, either sedentary or migratory species. Therefore it is suggested that diverse species inhabit Segara Anakan and different part of those areas has different species because different species commonly exhibit different habitat preferences. The objectives of this research were to determine the diversity and spatial distribution of fish species in Segara Anakan Cilacap. This research was a survey with the Cluster Random Sampling was implemented as sampling technique. Species diversity was analyzed descriptively, as well as the spatial distribution. The relationships between environmental parameters and spatial distribution were analyzed using Principal Component Analysis. Environmental factors such as temperature, pH, salinity, and water DO were measured. The result showed that Stolephorus indicus was the most dominant species in Segara Anakan. The spatial distribution of fishes in every sampling station was found fluctuated. The environmental factors and fish were found highly correlated.

DISTRIBUSI LONGITUDINAL DAN STRUKTUR POPULASI Rasbora spp. DI SUNGAI BANJARAN KABUPATEN BANYUMAS Titis Amelia; Windiariani Lestari; Agus Nuryanto
Scripta Biologica Vol 1, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Rasbora is a genus of freshwater fish of the family Cyprinidae. Three species belonging to this genus were found in Java Island; R. aprotaenia, R. lateristriata, and R. argyrotaenia. Rasbora inhabits clear running water with sandy and gravel bottom. This study aimed to determine the longitudinal distribution of Rasbora spp. based on their species richness and abundance at Banjaran River; to determine the population structure of Rasbora spp based on their body weight, standard and total length; and to identify interactions between physical-chemical factors with species richness and abundance of Rasbora spp. This research was a survey, with purposive random sampling technique applied to collect samples. The research divided the study sites into five stations based on their altitude and topography. The Cluster Analysis was calculated based on species richness and relative abundance of Rasbora spp., while the longitudinal distribution and the population structure were presented descriptively. The interaction between the physical-chemical factors and the species richness and abundance of Rasbora were analyzed using Principal Component Analysis (PCA). Thi study found two species of Rasbora, namely Rasbora argyrotaenia and Rasbora lateristriata with 80 and 2 individuals respectively. R. argyrotaenia was distributed longitudinally along Banjaran River. Meanwhile, R. latestriata was only found at the upstream. Based on the body weight, the population of R. argyrotaenia at the station three were found highest by the average body weight of 1–6 gr of a total 31 individuals. The maximum measurements of the standard length were observed at the station 3 with a range of diameter 4.0–5.9 cm of 30 individuals. The total length were also found at the station 3 with a range of measuremtnt 5.0–7.9 cm of 33 individuals. R. latestriata was found only two individual with body weights measured were 9 gr and 10 gr, the standard lengths were 7.6 and 8.5 cm, and the total lengths were 9.5 and 10.5 cm. The distribution of R. argyrotaenia was influenced mostly by pH, the speed of the water, and DO. The presence of R. lateristriata was influenced primarily by BOD and depth.

KARAKTERISASI BEBERAPA STRAIN GURAMI Osphronemus gouramy Lac. MENGGUNAKAN MARKA RAPD Anisa Kartika Sari; Agus Nuryanto; Agus Hery Susanto
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Giant gouramy, Osphronemus gouramy Lac. is a popular fish species in Indonesia, especially in Java and Sumatera as this freshwater fish species has a high economic value of stable price. Fish farmers in Bogor divide giant gouramy into six strains based on egg productivity, growth rate, and maximum weight of the adult. They are soang, jepang, blue saphire, paris, bastar, and porselin. These various strains lead to the need of study on the genetic relationship among them, which can be performed by the use of RAPD (Random Amplified Polymorphic DNA) marker. This study aims to determine primers producing consistent and polymorphic RAPD markers, determine specific RAPD markers, and know the genetic relationship among several giant gouramy strains. The strains used in this study are soang, jepang, and blue saphire. Survey method was applied employing purposive random sampling technique. Total genomic DNA was isolated using Genjettm genomic DNA purification kit (Fermentas), which was then used as template to amplify RAPD markers with primers OPA-07, OPA-09, OPA-11, OPA-20, OPAH-01, OPAH-08, OPAH-09, and OPAC-14. The variables examined were patterns and numbers of specific DNA fragments as the PCR amplification products. Selected primers were determined descriptively on the basis of specific DNA bands appearing on the agarose gel. Genetic diversity was predetermined by changing qualitative band pattern into quantitative binnary data. Genetic relationship was analyzed using cladistic method with PAUP software. The results showed that only five of the eight primers produce consistent and polymorphic RAPD markers, i.e. OPA-11, OPA-20, OPAH-1, OPAH-8, and OPAH-9. Specific RAPD markers which can be used to distinguish several gouramy strains are those amplified with OPA-20 of 786 bp, OPA-20 of 1,176 bp, OPAH-8 of 1,000 bp and OPAC-14 of 1,607 bp. Nervertheless, it was found that RAPD markers cannot be used to clearly determine genetic relationship among gouramy strains.

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