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Endang Widyastuti

Fakultas Biologi, Universitas Jenderal Soedirman

Author-ID : 3698273

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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PENGARUH PEMBERIAN MIKROBA EFEKTIF PRODUKTIF PLUS (MEP+) PADA MEDIUM BUDIDAYA IKAN NILA YANG DIBERI PAKAN FERMENTATIF TERHADAP KEPADATAN BAKTERI ASAM LAKTAT Nita Wulandari; Sukanto Sukanto; Endang Widyastuti
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Microbes Effective Productive Plus (MEP+) in fish culture has role as probiotics and decomposer. Application of MEP+ is done by adding MEP+ on culture medium of Tilapia and fish feed. Fish feed is fermentative feed with addition of different concentration of cassava peel flour. The aim of this research were to find out the influence of MEP+ administration in culture medium and in fermentative feed with addition cassava peel flour on the increasing density of lactic acid bacteria in culture medium and find out the highest density of lactic acid bacteria. The research was done experimentally, used Complete Randomized Design with treatment of MEP+ administration in culture medium with fermentative feed cassava peel flour addition of 25%, 50%, 75% and without MEP+ administration on culture medium with fermentative feed cassava peel flour addition of 25%. The data obtained were analyzed using a variety analysis. The result showed that MEP+ administration on culture medium and in fermentative feed cassava peel flour addition did not influence the increasing density of lactic acid bacteria and total density of lactic acid bacteria in culture medium was not different inter treatment.

HUBUNGAN PERBANDINGAN TOTAL NITROGEN DAN TOTAL FOSFOR DENGAN KELIMPAHAN CHRYSOPHYTA DI PERAIRAN WADUK PANGLIMA BESAR SOEDIRMAN, BANJARNEGARA Faralenggi DM Putri; Endang Widyastuti; Christiani Christiani
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Nitrogen (N) and phosphorus (P) affect the growth of phytoplankton in the waters. Nitrogen is an important part in forming protein in organisms, while phosphorus is the most essential element for growth and contributes in protein formation and cell metabolism in organisms. The activities from the catchment area that enter into the P.B Soedirman Reservoir can affect both concentration of N and P. The comparison total nitrogen (TN) and total phosphorus (TP) is the limiting factor to affect the growth of phytoplankton. Chrysophyta is one of division from phytoplankton that has function as an important component in the food chain and become as natural feed for fish. This research showed the abundance of Chrysophyta, relations of TN with the abundance of Chrysophyta; TP with the abundance of Chrysophyta; TN/TP with the abundance of Chrysophyta; TN, TP, TN/TP with the abundance Chrysophyta in the P.B Soedirman Reservoir, Banjarnegara Regency. This research used survey method with purposive sampling technique on 7 observation stations with 3 repeating. The relation of TN/TP with the abundance of Chrysophyta were analyzed used correlation regression - correlation analyzis. The results of the research showed that total abundance Chrysophyta was between 339-9570 ind/l, relation between TN/TP with the abundance of Chrysophyta with the degree was weak to very strong correlation. The most affection correlation was shown by of TN, TP, TN/TP collectively with the abundance of Chrysophyta with coefficient value (r) of 0,890 with the strength of the relation of 79,3%. The regression analysis of TN, TP, TN/TP collectively with the abundance of Chrysophyta was shown by the equation Y= – 80987,801 – 8499,931TN + 1,684TP + 406,373TN/TP.

KELIMPAHAN CHRYSOPHYTA PADA MEDIA BUDIDAYA IKAN NILA YANG DIBERI PAKAN FERMENTASI DENGAN PENAMBAHAN TEPUNG KULIT UBI KAYU DAN PROBIOTIK Retno Ambarwati; Endang Widyastuti; Dwi Sunu Widyartini
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Chrysophyta is also known as golden-yellow algae because of the yellow dominant pigment of carotene and xanthophyll. This study aimed to determine species richness and abundance of Chrysophyta in the Tilapia culture media which was fed with the addition of cassava peel flour and probiotic. The method applied in this study was the experimental method with 4 treatments, i.e.: the use of fermented feed with the addition of cassava peel flour (0%, 25%, 50%, and 75%) and probiotic in Tilapia culture media. Each treatment was replicated 4 times. Sampling was carried out 6 times at intervals of 2 weeks. The observed parameters were the main parameters, i.e.: the number of Chrysophyta species and individuals; and supporting parameters, i.e.: water temperature, pH, TDS, TSS, NO3, NO2, BOD5, DO, and total of phosphate. Species richness and abundance of Chrysophyta data were analyzed using cluster analysis based on Bray-Curtis similarity coefficient. The analysis continued with Similarity Percentages (SIMPER) analysis to determine the contribution of species to the similarity index in each group or to dissimilarity index amongst groups. The results showed that the species richness found in the Tilapia media culture which was fed with the addition of cassava peel flour and probiotic consist of 20 species belonging to a class namely Bacillariophyceae. Abundance of Chrysophyta obtained was ranged from 5.160-13.292 individuals/liter. The cluster analysis showed that Chrysophyta amongst treatments have a quite high similarity level (> 50%) was ranged between 65.56% -83.99%. Contributions of species which contribute the highest similarity index were Diatoma vulgare (49.80%), Navicula brachysira (70.50%) and Navicula platystoma (82%).

KELIMPAHAN CHLOROPHYTA PADA MEDIA BUDIDAYA IKAN NILA YANG DIBERI PAKAN FERMENTASI DENGAN PENAMBAHAN TEPUNG KULIT UBI KAYU DAN PROBIOTIK Heni Andriyani; Endang Widyastuti; Dwi Sunu Widyartini
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Chlorophyta is autotroph organisms which has an important role in fresh waters as one of the largest algae division. This study aimed to determine the abundance and similarity structure of Chlorophyta in Tilapia culture media which was given fermented feed with the addition of cassava peel flour and MEP+ probiotic. This study used four treatments with four replications: (A) fermented feed without addition of cassava peel flour or 0% and MEP+ probiotic administration in media, (B) 25%, (C) 50% and (D) 75%. Sampling was carried out 6 times at intervals of 2 weeks. The main parameters were the number of Chlorophyta species and individuals,while supporting parameters were physical (temperature and TDS) and chemical (DO, BOD, pH, NO3, NO2 and total of PO4). The abundance of Chlorophyta data were analyzed descriptively and its similarity structure were analyzed using Cluster analysis. Then, continued with SIMPER analysis to determine the contribution of species to abundance similarity with PRIMER-E v.5 software. Analysis results showed that the abundance of Chlorophyta consists of 33 species with the average number of 10.412 individuals/liter. Cluster analysis results based on Bray-Curtis similarity index had a quite high similarity and it ranged between 57.79% -68.84%. SIMPER analysis results showed that the species which given highest contribution were Kircheneriella lunaris (31,03%), Selenastrum sp. (21,69%), and Gonatozygon monotaenium (12,96%).

POPULASI BAKTERI ASAM LAKTAT PADA BUDIDAYA IKAN NILA YANG DIBERI PAKAN FERMENTASI LIMBAH PERTANIAN DENGAN SUPLEMEN ENCENG GONDOK DAN PROBIOTIK Andhika Agus Setyawan; Sukanto Sukanto; Endang Widyastuti
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

The aim of this study were to determine the effect of fermented agricultural wastes feeding with supplement of water hyacinth and MEP+ probiotic towards the increasing population of intestinal Lactid Acid Bacteria (LAB) in Tilapia and to know the highest population of lactic acid bacteria after fermented agricultural waste feeding with water hyacinth supplements and MEP+ probiotic. Independent variable in this study was fermented feed with the addition of different water hyacinth and MEP+ probiotics. The experimental study was continued using completely randomized design (CRD). The dependent variable in this study was the number density of Lactobacillus bacteria in the gut of Tilapia. The main parameters measured were the number of LAB. Proponent parameters were namely Tilapia weight gain, physical and chemical conditions of the water. The results showed that feeding fermentative effected in increasing the population of lactic acid bacteria in the gut of Tilapia. Fermentative feed supplement agricultural waste with water hyacinth as much as 10% and MEP+ probiotic was the best treatment with LAB population density in Tilapia intestine with the average of 5.85 x 106 CFU /g.

Co-Authors Andhika Agus Setyawan Christiani Christiani Dwi Sunu Widyartini Faralenggi DM Putri Heni Andriyani Nita Wulandari Retno Ambarwati Sukanto Sukanto

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