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All Journal Scripta Biologica BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA)
Setyawati, Endang Ariyani
Fakultas Biologi, Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Public Health Veterinary

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Eliminasi Endosimbion Wolbachia sp. pada Nyamuk Aedes albopictus dengan Antibiotik Tetrasiklin Endang Srimurni Kusmintarsih; Darsono Darsono; Edy Riwidiharso; Rokhmani Rokhmani; Trisnowati Budi Ambarningrum; Endang Ariyani S
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Volume 17 Nomor 2 Desember 2021
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/blb.v17i2.4249

Abstract

Dengue Hemorrhagic Fever (DHF) is a disease caused by the dengue virus which is transmitted through the bite of the Aedes mosquito. Aedes aegypti, which is known as the primary vector of dengue virus, is naturally not infected by Wolbachia sp. endosymbiont, while Ae. albopictus which is a secondary vector naturally infected with Wolbachia sp. The Wolbachia sp. known to inhibit the transmission of Dengue virus, to study the mechanism, it is necessary to eliminate Wolbachia sp. from Ae. albopictus, then infects the Ae. albopictus with the Dengue virus. The aim of the study was to determine the ability of tetracycline antibiotics to eliminate Wolbachia sp. from the Ae. albopictus mosquito. Ae. albopictus eggs was obtained in the Ciamis area by survey method using ovitrap which was installed outside the house. The Ae. albopictus eggs are then incubated in the laboratory and reared until they become adult mosquitoes. Mosquitoes were treated with sugar feeding which had been given tetracycline 0.25mg/ml every two days alternated with blood feed. Detection of the presence of Wolbachia sp. on mosquitoes carried out in first to third generations by the polymerase chain reaction (PCR) method using Wsp-specific primers. The results showed that the first to third generation mosquitoes were still infected with Wolbachia sp. This shows that the dose of tetracycline antibiotics used has not been able to eliminate Wolbachia sp. from the Ae. albopictus mosquito.
DISTRIBUSI GEOGRAFIS TUNGAU PARASIT NYAMUK Aedes sp. DI DAERAH ENDEMIS DEMAM BERDARAH DENGUE DI KABUPATEN BANJARNEGARA Arthur Shepta Laksono; Bambang Heru Budianto; Endang Ariyani Setyowati
Scripta Biologica Vol 1, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (699.063 KB) | DOI: 10.20884/1.sb.2014.1.2.436

Abstract

Parasitic mites are known as the natural enemy of Aedes sp. that can potentially infect any life stages of the Aedes sp. The parasitic mites’ ability to infect Aedes sp. influence the distribution pattern of parasitic mites. The widespread distribution of Aedes sp. is expected to affect the distribution of parasitic mites. The aims of this study were to determine the taxonomic family of parasitic mites that infected Aedes sp. larvae and to determine the geographical distribution patterns of parasitic mites of Aedes sp. in the endemic area of Dengue Hemorrhagic Fever (DHF) in Banjarnegara District. This study used larvae of Aedes sp. taken from the endemic area of DHF in Banjarnegara District. The study sites were in the Parakancanggah Village, Kutabanjar Village, Krandegan Village, and Sokanandi Village. This study used survey method with purposive sampling technique. Variable observed in this research was the distribution pattern of the parasitic mite of Aedes sp. larvae. Parameter observed included the family and the individual number of parasitic mites on each mosquito larvae, the average value, and the variance. Data were analyzed using mean value and the variance so that the distribution pattern can be determined. The level of parasitic mites’ distribution was analyzed using K' index negative binomial distribution. Results showed that 30 individual of parasitic mite were found from 1429 samples of examined Aedes sp. larvae. The identification result were five families of parasitic mites: Pionidae, Histiostomatidae, Hydryphantidae, Hydrachnidae, and Arrenuridae. The distribution pattern of the parasitic mites was regular, and the highest value of K’ index negative binomial distribution was 1,3225, in the Krandegan Village.
Co-Authors Arthur Shepta Laksono Bambang Heru Budianto Darsono Darsono Edy Riwidiharso Endang Srimurni Kusmintarsih Rokhmani Rokhmani Trisnowati Budi Ambarningrum