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All Journal Scripta Biologica Indonesian Journal of Applied Research (IJAR) International Journal on Education Insight
Diah Asta Putri
Universitas Ahmad Dahlan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Languange, Linguistic, Communication & Media Mathematics Other

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SERBUK KAYU JATI (Tectona grandis Linn. F.) SEBAGAI SUBSTRAT ALTERNATIF UNTUK PRODUKSI ENZIM SELULASE Diah Asta Putri; Taufik Hidayat
Scripta Biologica Vol 6, No 2 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

Selulase merupakan salah satu enzim yang digunakan secara luas dalam industri dan kebutuhannya semakin meningkat. Untuk memenuhi permintaan industri, dibutuhkan medium berbiaya rendah untuk produksi selulase. Pemanfaatan limbah dapat menyediakan substrat alternatif dan membantu dalam memecahkan masalah polusi. Selulase dapat dihasilkan melalui proses fermentasi dengan bantuan mikroorganisme yang ditumbuhkan pada substrat yang mengandung selulosa. Penelitian ini menggunakan limbah dari pengolahan kayu jati yaitu serbuk gergaji kayu jati. Jumlah serbuk gergaji dari kayu jati melimpah dan pemanfaatannya belum optimal. Serbuk gergaji kayu jati diketahui memiliki kadar selulosa sebesar 46,5%. Kadar selulosa yang tinggi tersebut menjadikan serbuk gergaji kayu jati berpotensi sebagai substrat dalam produksi enzim selulase. Penelitian ini bertujuan untuk menentukan waktu inkubasi dan moisture content substrat yang optimal bagi Aspergillus niger untuk memproduksi selulase dari substrat serbuk kayu jati. Uji enzim dilakukan dengan metode CMCase. Hasil penelitian menunjukkan bahwa waktu inkubasi yang optimal untuk produksi enzim selulase oleh Aspergillus niger adalah 48 jam dengan kadar selulase 266,303 mg/L sedangkan moisture content substrat yang optimal adalah 50% dengan kadar selulase  197,394 mg/L.
KARAKTERISASI KROMOSOM UDANG GALAH (Macrobrachium rosenbergii) POPULASI SIRATU DAN MAHAKAM Diah Asta Putri; Nurul Suwartiningsih
Scripta Biologica Vol 6, No 2 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Giant freshwater prawn is one of Indonesia's freshwater prawn that has advantages such as fast growth and good meat quality. The continuous crossing of giant freshwater prawn that are closely related causes a decrease in its genetic diversity. In addition, the domestication process can also influences changes in the number and shape of chromosomes. This study is to determine the chromosome characters of domestication giant freshwater prawn (Siratu)  and those derived from natural catches (Mahakam) which are cultured in the Unit Kerja Budidaya Air Payau Balai Budidaya Udang Galah (UKBAP BBUG) Samas. Research on the characterization of chromosome of giant freshwater prawn from both natural and domesticated populations needs to be done to obtain genetic information, especially chromosome characters. This information is useful to further support the process of breeding and for the certification process of giant freshwater prawn. Chromosome length measurements were carried out using the Image Raster 3 program. The shape of the chromosomes was determined by following the classification of Levan et al. (1994) based on the Sentromer Index Value obtained. The results showed that the number of chromosomes in the population of Siratu and Mahakam were 2n = 118. The giant freshwater prawn of Siratu population had the karyotype formula 2n = 118 = 94 m + 24 sm, while the giant freshwater prawn Mahakam population had the karyotype formula 2n = 118 = 118 m.
Developing digital module for human literacy and technology literacy Dody Hartanto; Puguh Wahyu Prasetyo; Ika Maryani; Ega Asnatasia Maharani; Intan Puspitasari; Diah Asta Putri; Oktira Roka Aji; Wachid Eko Purwanto
International Journal on Education Insight Vol. 1 No. 1 (2020)
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.941 KB) | DOI: 10.12928/ijei.v1i1.2086

Abstract

In the era of industrial revolution 4.0, many human activities depend on the gadget and the internet connection. We can observe the daily activities of human life right now. When they wake up in the morning, they usually start by using their handphones or gadget. This condition motivates educators to build a digital module in order to use the positive point of view of the Industry Revolution 4.0 era. In this chance, the researchers develop two topics that are very important for human life in Industry Revolution 4.0, whicht are, human literacy and technology literacy. Both of the topics were derived into two-courses. The course of development of students (Perkembangan Peserta Didik) will be representing the human literacy topics and the course of introduction to Biotechnology (pengantar bioteknologi) will be representing the technology literacy topic. The research is research and development. Furthermore, the products of this research are flipbooks that were uploaded on the Learning Management System managed by Universitas Ahmad Dahlan.
Banana Endophytic Fungi Producing Antagonistic, Antioxidant, and Antibacterial Bioactive Metabolites Oktira Roka Aji; Diah Asta Putri; Ririn Kholifatu Yuliani; Nur Malika Ilma; Sutan Nur Chamida Tri Astuti
Indonesian Journal of Applied Research (IJAR) Vol. 7 No. 1 (2026): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v7i1.721

Abstract

The increasing prevalence of antibiotic-resistant pathogens and the environmental hazards associated with synthetic agrochemicals have intensified the search for natural bioactive agents. This study isolated and identified endophytic fungi from banana (Musa spp.) and evaluated their antimicrobial and antioxidant activities. Endophytic fungi were isolated from leaf and stem tissues using a surface-sterilization method, followed by culture purification. Antagonistic activity was assessed through dual culture assays, while molecular identification was performed using ITS rDNA sequencing. Five isolates were obtained, and isolate J2 exhibited clear antifungal activity against Fusarium oxysporum. Sequence analysis showed that J2 is closely related to Talaromyces aculeatus. Fungal metabolites were extracted using methanol and ethyl acetate, and the extracts were tested for antioxidant activity (DPPH assay), total phenolic content, and antibacterial activity against Escherichia coli and Bacillus cereus. The methanol extract exhibited stronger antioxidant activity (IC₅₀: 3285.41 ± 80.06 ppm), while the ethyl acetate extract showed higher phenolic content (45.04 ± 9.02 mg GAE/g) and superior antibacterial effects. These results highlight T. aculeatus as a promising natural source of multifunctional bioactive compounds.
Co-Authors Ega Asnatasia Maharani Hartanto, Dody Ika Maryani Nur Malika Ilma Nurul Suwartiningsih Oktira Roka Aji Puguh Wahyu Prasetyo, Puguh Ririn Kholifatu Yuliani Sutan Nur Chamida Tri Astuti Taufik Hidayat Wachid Eko Purwanto