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All Journal Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Scripta Biologica
Sugiharto Sugiharto
Fakultas Biologi Universitas Jenderal Soedirman Jl.DR. Soeparno No.63 Karang Wangkal Purwokerto
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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PERKEMBANGAN POST-LARVA IKAN NILEM Osteochilus hasselti C.V. DENGAN POLA PEMBERIAN PAKAN BERBEDA Didi Humaedi Yusuf; Gratiana E Wijayanti; Sugiharto Sugiharto
Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1177.287 KB) | DOI: 10.20884/1.sb.2014.1.3.40

Abstract

Failure of adaptation in feed utilization can result in larval mortality. Post-larval stage is a critical period of fish development in which transition from internal feeding to external feeding occurs. This study aimed to determine the growth and survival of hard-lipped barb (Osteochilus hasselti C.V.) post-larvae fed with different feeding patterns. The experiment was conducted on an experimental basis with a completely randomized design (CRD). The treatments consisted of (P1) 100% Spirulina sp., (P2) 75% Spirulina sp. + 25% pellet powder, (P3) 50% Spirulina sp. + 50% powder pellet, (P4) 25% +75% sp Spirulina pellet powder, and (P5) 100% powder pellets. Day 1 post-hatching larvae were reared in aquaria with density of 17 fish / L for 8 weeks. The larvae were fed as much as 5% of the total weight of biomass. Post-larval development was evaluated by observing the development of the larvae morphology, the body length (every week), the biomass weight (every 2 weeks), and calculating their survival rate at week 8. Morphological data were analyzed descriptively. Data on body length, biomass weight and survival were analyzed using two-way ANOVA followed by LSD test. The results showed that the feeding pattern significantly affected the development of post-larvae (p<0.05). The most advance post-larval development was obtained from the post-larvae fed with 100% pellet powder. In this group, morphological characters of post-larvae in the first week were the mouth start opening, the skin was pigmented, and gall bladder in bi-lobes shaped, caudal fin and dorsal fins were differentiated. Completion of fins development was achieved by the third week when the post-larvae have adult morphological character. The highest body length and biomass weight of the post-larvae was obtained in the group fed with100% of pellets and the highest survival rate was found in the group fed with combination of 50% Spirulina sp. + 50% pellet powder (P3).
Induksi Pematangan Gonad dengan Ovaprim-C: untuk Penyediaan Benih Belut Sawah (Monopterus albus) Berkelanjutan Priyo Susatyo; Sugiharto Sugiharto; Elly Tuti Winarni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 3 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.3.247

Abstract

Rice eel (Monopterus albus Zuiew), have economical value is high in Banyumas, national to international markets, but rarely is grown intensively, the seed is still dependent on the catch. Reproduction of swamp eel unlike other fish species in the presence of phase teleostei  intersex / hermafroditus and in nature only spawn once a year. This study will seek to accelerate the scale seed production with natural canvas with several techniques triggers lust and sex organ maturation eels based preparedness aspects of reproductive anatomy, hormones estradiol and testosterone profiles and the intrinsic need for hormonal regulation and the mechanism for the reproductive cycle of the eel rice. In the first year aims to determine (1) the profile of estradiol and testosterone hormonal parent during the natural reproductive cycle, (2) histologic gonadogenesis (development of the testes and ovaries) eel rice during the natural reproductive cycle was evaluated by measuring fecundity. Results: In the control group, body weight gradually decreased during the study. Unlike the control group, the treatment groups were observed every two weeks showed an increase in body weight; Results of the study, in the control group (A0), the value of female eels GSI average of the first two weeks until the fourth two weeks is 0.67 % and 1.78%. While the treatment group A1 was 1.42% - 4.28% (DM-1 s / d DM-4), for the treatment A2 GSI average value is 2.52% - 7.05%; fecundity eggs increased during induction period; calibration titer two types of hormones in the first two weeks to the fourth two weeks showed improvement when compared with the control group; histological profile of rice eel gonad after induction of ovaprim-C show that up to the fourth two weeks, gonad has reached late-stage yolk globule (advanced primary oocyte), even the most mature oocyte has reached the stage: in male eels up to the fourth two weeks has reached the stage of spermatozoa
Co-Authors Didi Humaedi Yusuf Elly Tuti Winarni Gratiana Ekaningsih Wijayanti Priyo Susatyo