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All Journal Jurnal Teknologi Dan Industri Pangan Forum Pasca Sarjana
A. Winny Sanjaya
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Agriculture, Biological Sciences & Forestry Education Industrial & Manufacturing Engineering

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PENGARUH BERBAGAI KONDISI PREPARASI DAN PENYIMPANAN SUSU FORMULA PADA PERTUMBUHAN SPORA Bacillus cereus DAN Clostridium perfringens [The growth of Bacillus cereus and Clostridium perfringens spores under a variety of preparation and storage condition] Purwanti Maya; Mirnawati Sudarwanto; Winiati P. Rahayu; A. Winny Sanjaya
Jurnal Teknologi dan Industri Pangan Vol. 20 No. 1 (2009): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The aim of this study was to evaluate the ability of Bacillus cereus and Clostridium perfringens spores to survived in different preparation temperature (25, 35, and 70°C for B. cereus and 25, 45, 70ºC for C. perfringens) and modified storage condition (with 50 and 75% humidity; opened, closed and opened twice a day during period of observation) of powdered milk  formula.  The spores of B. cereus ATCC 13061 and         C. perfringens CP-1 artificially  contaminated to the milk formula. Results showed that B. cereus and C. perfringens spores were germinated and growth in reconstituted milk formula.  Bacteria population increased ≥1 log within 3 hours at room temperature (28-29ºC).  The spores of both bacteria survived from dry condition of powdered milk formula, like when aw of the formulas increased cause of storage condition.
Through a nutritional improvement project, Bogor District had donated powdered milk formula (PMF) for severe malnutrition children under five years.  Powdered milk formula was assumed as an unsterilized product, because it might contain spore forming bacteria.  To evaluate the bacterial contaminant of home prepared milk formula, fourty eight samples of PMF, and 50 samples of reconstitution formula, drinking water, drinking equipment, and hand were taken proporsionally from the mothers in 10 Heal Maya Purwanti; Mirnawati Sudarwanto; Winiati P. Rahayu; A. Winny Sanjaya
Forum Pasca Sarjana Vol. 31 No. 4 (2008): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Through a nutritional improvement project, Bogor District had donated powdered milk formula (PMF) for severe malnutrition children under five years.  Powdered milk formula was assumed as an unsterilized product, because it might contain spore forming bacteria.  To evaluate the bacterial contaminant of home prepared milk formula, fourty eight samples of PMF, and 50 samples of reconstitution formula, drinking water, drinking equipment, and hand were taken proporsionally from the mothers in 10 Health Community Centre around Bogor District.  Samples were analyzed for aerobic microbe, Bacillus cereus, Clostridium perfringens, and the potential of enterotoxins production.  All of the donated PMF samples exhibiting a total aerobic count of <104 CFU/g (mean 1.2 x 102 CFU/g) and B. cereus count of <103 CFU/g (mean 3.0 x 102 CFU/g for PMF containing this bacterium).  Improper home storage promote the growth of aerobic microbe and B. cereus.  Improper preparation promote the growth of B. cereus which present initially at low level (mean 7.8 x 10 CFU/g) and became 1.6 x 103 CFU/ml after the reconstitution.  Clostridium perfringens emerge at 4 samples (mean 1.5 x 10 CFU/ml) after the reconstitution.  Several isolate of B. cereus (4 from PMF, 13 from opened PMF, and 2 from reconstution formula) showed a possibility to produce diarrheagenic enterotoxin while the isolate of C. perfringens did not shown it.   Keywords: powdered milk formula, B. cereus, C. perfringens, diarrheagenic enterotoxin, preparation and storage
Co-Authors Maya Purwanti Mirnawati Sudarwanto Purwanti Maya Winiati P. Rahayu