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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Indonesian Journal of Biotechnology Jurnal Kimia Terapan Indonesia
Sanro Tachibana
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology

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Journal : Indonesian Journal of Biotechnology

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Decolorization of Remazol Briliant Blue R by Laccase from White Rot Fungus Polyporus sp. S133 Tony Hadibarata; Sanro Tachibana
Indonesian Journal of Biotechnology Vol 14, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.014 KB) | DOI: 10.22146/ijbiotech.7811

Abstract

The decolourization of the recalcitrant dye RBBR by the culture filtrate of Polyporus sp. S133 and its isolatedlaccase was investigated. The laccase alone decolorized RBBR. A small molecular weight redox mediator (HBT) wasnecessary to increase the decolorization. The purified laccase totally decolorized the dye of 200 mg l-1 initialconcentration of RBBR when only 1.5 U ml-1 of laccase was used in the reaction mixture. The effects of differentphysicochemical parameters were tested and optimal decolorization rates occurred at pH 5 and at a temperature of 50°C. The effect of surfactants on the decolourization of RBBR was tested with Tween 80, Tween 20, and Brij 35. It wasdemonstrated that Tween 80 was inhibiting substrate for the decolorization while Tween 80 and Brij 35 was noinhibiting effect for the decolorization. Provided that all of the condition is included, it is suggested that laccase maybe suitable for the wastewater treatment of similar anthraquinone dyes.Keywords: Decolorization; Laccase; Remazol Brilliant Blue R (RBBR); Polyporus sp. S133
Co-Authors Amalia Indah Prihantini Anastasia Wheni Indrianingsih Kazutaka Itoh Leonardus B.S. Kardono Masaya Mitani Nina Artanti Tata Darma Tony Hadibarata Yutaka Tamai