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All Journal Jurnal Ilmu Dasar BERITA BIOLOGI Jurnal Kimia Terapan Indonesia
Hendro Santoso M
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Materials Science & Nanotechnology Mathematics

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PENGARUH PENAMBAHAN JUMLAH INOKULUM DAN DI-KALIUM HIDROGEN FOSFAT PADA FERMENTASI PRODUKSI DEKSTRAN Triantarti Triantarti; Hendro Santoso M
BERITA BIOLOGI Vol 7, No 3 (2004)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i3.1060

Abstract

Dextran production is conducted by fermentation by using Leuconostoc mesenteriodes wich produces dextransucrase enzyme.Sucrose is converted to dextran by dextransucrase. Sucrose is a main carbon source in dextran fermentation. Hence, sugar cane juice mainly contains sucrose is potential material for dextran fermentation. The effect of inoculum concentration added at the beginning of fermentation of di-potassium hydrogen phosphate concentration in the medium were studied. L.mesenteroides B-512F was used. The results showed that there were no effect on optimum growth and dextran production when the inoculum concentration added at 1% and 5%(w/v). The only difference was inoculum at 1% (w/v) delaying the growth and dextran formation in comparison to the addition of 5%(w/v) inoculum. The optimum growth and dextran production were affected by di-potassium hydrogen phosphate concentration in the medium (0,5, 1,0 and 1,5% w/v).The growth was highest at di-potassium hydrogen phosphate concentration 1,5 % w/v. On the otherhand, dextran production was lower compared to the other treatments.
KAJIAN PENGARUH SEL IMOBIL Arthrobacter NRRL B-3728 TERHADAP AKTIVITAS DAN STABILITAS ENZIM GLUKOSA ISOMERASE Triantarti Triantarti; Hendro Santoso M
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1224

Abstract

This project focused on the immobilization of glucose isomerase (GI) from Arthrobacter B-3728. The whole cells immobilization technique using glutaraldehyde and gelatine type B220 was used in this research. The optimum time for harvesting Arthrobacter cells was determined before immobilization.The Arthrobacter cells were harvested after 56-72 h fermentation when the GI activity reaching 0.515-0.603 U/ml broth. The best treatment for cell immobilization was found using 5% gelatine when the GI activity reaching 0,888 U/ g immobilized cells. The optimum pH was not changed (pH 8) but the sensitivity to the pH was changed for immobilized cells compared to the free cells. However, the stability of the immobilized cells was lower compared to the free cells for long isomerization process. Further research are still needed for the development of immobilization technique for Arthrobacter B-3728.
Effect of Substituting Pure Sucrose by Sugarcane Juice as Carbon Source on the Fermentation of Dextran Production Triantarti Triantarti; Hendro Santoso M
Jurnal ILMU DASAR Vol 8 No 2 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (62.682 KB)

Abstract

Sucrose is a carbon source for dextran fermentation and it is also used as a substrate of dextransucrase enzyme for producing dextran.  Sugar cane juice containing sucrose as a main sugar, hence it is potential to be used as a cheap medium for dextran fermentation.  This research was conducted to study the dextran fermentation using sugar cane juice as a medium. Two main experiments were done in this research. The first experiment has been carried out to determine the optimum medium composition for dextran fermentation using pure sucrose as a carbon source by variations on type and concentrations of yeast extract and buffering minerals.  The second experiment was conducted to determine the effect of substituting pure sucrose in the fermentation medium by sugar cane juice. Fermentation was conducted at static condition, room temperature and 16-20 h fermentation time. The results showed that the optimum conditions for dextran fermentation using pure sucrose were sucrose 20%, yeast extract 0.75% (technical grade yeast extract was able to be used) and K2HPO4 minerals for buffering medium. Dextran production was able to reach 51 mg/g medium. The optimum medium composition and fermentation conditions were used as a control medium.  In the second experiment, pure sucrose in the control medium was substituted by sugar cane juice with variations of 0; 50, 75 and 100%. Technical grade yeast extract was still added at 0.75%.  The result showed that the higher sugar cane juice concentration the lower dextran production in the fermentation.  On the other hand, medium fermentation containing 100% sugar cane juice without yeast extract was able to produce 45 mg dextran/g medium, which was not significantly different to dextran production from control medium.  This experiment showed that sugar cane juice was a potential material as a cheap carbon source for dextran fermentation
UJI BANDING METODE ANALISIS KONVENSIONAL DAN INSTRUMENTAL UNTUK PENENTUAN KALSIUM DAN FOSFOR Hendro Santoso M
Jurnal Kimia Terapan Indonesia Vol 7, No 1-2 (1997)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2623.998 KB) | DOI: 10.14203/jkti.v7i1-2.226

Abstract

Analysis of calcium and phosphor has been done by using conventional and instrumental methods. Lorenz gravimetric method was employed to determine phosphor, whereas permanganometric methods used to determine calcium. Spectronic molibdovanadophosphate and atomic absorption spectrophotometric methods were used as control for phosphor and calcium respectively. Samples analysed in this study were molasses and driedfaeces.Results show that there is no significant effect on analysis phosphor by using both Lorenz gravimetric method and its control. Similarly, permanganometrlc methods result in the same value with its control.
Co-Authors Triantarti Triantarti