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All Journal Jurnal Ilmu Dasar BERITA BIOLOGI Jurnal Kimia Terapan Indonesia
Hendro Santoso M
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Materials Science & Nanotechnology Mathematics

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Journal : BERITA BIOLOGI

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PENGARUH PENAMBAHAN JUMLAH INOKULUM DAN DI-KALIUM HIDROGEN FOSFAT PADA FERMENTASI PRODUKSI DEKSTRAN Triantarti Triantarti; Hendro Santoso M
BERITA BIOLOGI Vol 7, No 3 (2004)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i3.1060

Abstract

Dextran production is conducted by fermentation by using Leuconostoc mesenteriodes wich produces dextransucrase enzyme.Sucrose is converted to dextran by dextransucrase. Sucrose is a main carbon source in dextran fermentation. Hence, sugar cane juice mainly contains sucrose is potential material for dextran fermentation. The effect of inoculum concentration added at the beginning of fermentation of di-potassium hydrogen phosphate concentration in the medium were studied. L.mesenteroides B-512F was used. The results showed that there were no effect on optimum growth and dextran production when the inoculum concentration added at 1% and 5%(w/v). The only difference was inoculum at 1% (w/v) delaying the growth and dextran formation in comparison to the addition of 5%(w/v) inoculum. The optimum growth and dextran production were affected by di-potassium hydrogen phosphate concentration in the medium (0,5, 1,0 and 1,5% w/v).The growth was highest at di-potassium hydrogen phosphate concentration 1,5 % w/v. On the otherhand, dextran production was lower compared to the other treatments.
KAJIAN PENGARUH SEL IMOBIL Arthrobacter NRRL B-3728 TERHADAP AKTIVITAS DAN STABILITAS ENZIM GLUKOSA ISOMERASE Triantarti Triantarti; Hendro Santoso M
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1224

Abstract

This project focused on the immobilization of glucose isomerase (GI) from Arthrobacter B-3728. The whole cells immobilization technique using glutaraldehyde and gelatine type B220 was used in this research. The optimum time for harvesting Arthrobacter cells was determined before immobilization.The Arthrobacter cells were harvested after 56-72 h fermentation when the GI activity reaching 0.515-0.603 U/ml broth. The best treatment for cell immobilization was found using 5% gelatine when the GI activity reaching 0,888 U/ g immobilized cells. The optimum pH was not changed (pH 8) but the sensitivity to the pH was changed for immobilized cells compared to the free cells. However, the stability of the immobilized cells was lower compared to the free cells for long isomerization process. Further research are still needed for the development of immobilization technique for Arthrobacter B-3728.
Co-Authors Triantarti Triantarti