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Kunti Tirtasari
Fakultas Kedokteran Hewan Universitas Nusa Tenggara Barat
Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Education Public Health Social Sciences

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PROTEKSI MINYAK BUAH MERAH (Pandanus conoideus Lam.) TERHADAP JUMLAH FOLIKEL YANG TERPAPAR PLUMBUM Novarina Sulsia Ista’in Ningtyas; Kunti Tirtasari; Alfiana Laili Dwi Agustin
JURNAL SANGKAREANG MATARAM Vol. 5 No. 4 (2019): Desember 2019
Publisher : SANGKAREANG

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Paparan timbal dapat menyebabkan gangguan pada organ reproduksi, salah satunya adalah abnormalitas folikulogenesis. Folikulogenesis penting untuk menghasilkan ovum yang siap untuk fertilisasi. Gangguan pada system reproduksi salah satunya adalah paparan logam berat yang menyebabkan radikal bebas. Penelitian ini bertujuan untuk mengetahui pengaruh buah merah sebagai proteksi terhadap paparan plumbum pada jumlah folikel mencit betina. Penelitian dilakukan selama 14 hari perlakuan dengan dengan perlakuan dengan P0 (control negative), P1 (control positif tanpa pemberian minyak buah merah dan dipapar plumbum dengan dosis 0,011mg/20g BB), P2 (0,3 ml minyak buah merah dan dipapar plumbum dengan dosis 0,011mg/20g BB), P3(0,8 ml minyak buah merah dan dipapar plumbum dengan dosis 0,011mg/20g BB). Perhitungan jumlah folikel didapatkan dengan membaca preparat HE ovarium. Penelitian ini menunjukkan adanya pengaruh pemberian minyak buah merah sebagai protectan terhadap jumlah folikel yang dipapar plumbum.
ISOLATION AND IDENTIFICATION OF BARTONELLA SPP. FROM BAT BLOOD AT TANJUNG RINGGIT CAVE ON EAST LOMBOK Andreyani Attamimi; Rima Nurmayani; Erni; Kunti Tirtasari; Kholik
JURNAL SANGKAREANG MATARAM Vol. 4 No. 3 (2018): September 2018
Publisher : SANGKAREANG

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Many research explained that bats are an important reservoir for Bartonella spp. On Lombok Island has many caves for breeding house of bats. The purpose of this research is to isolate and identify Bartonella spp. at Tanjung Ringgit Cave on East Lombok. An Observational survey with explorative laboratory was carried out on Tanjung Ringgit bat cave during May 2018. The bats were captured by net trap and blood samples were collected by puncturing the axillaries vein of bats for blood smears and examination of the presence of intraerythrocytic corpuscles. Each of blood samples was dipped in sterile BHI and they were incubated over the night. After 24 hours the samples were inoculated in Blood Agar under aerobic conditions at Laboratory of Public Health and calibration, Province of Nusa Tenggara Barat. The colonies were purified and characterized by biochemical methods. The result of blood smears by showed that 3 (30%) of 10 bat blood samples were found intraerythrocytic corpuscles. The morphological colonies of bacteria of the three bat blood that found intraerythrocytic corpuscles on Blood Agar are Heterogenic colonies, small regular to irregular larger forms and No hemolysis on blood agar. The result of the biochemical test showed that bacteria was non fermentative, urease, and indole negative. It also did not utilize citrate and Triple Sugar Iron (TSI) showed alkaline. Bartonella-like was isolated from bat blood at Tanjung Ringgit Cave on East Lombok besides on blood smears examination, The morphological colonies on Blood Agar and biochemical test of isolated bacteria of this research.
ANTIMICROBIAL RESISTANCE OF ESCHERICHIA COLI THAT ISOLATED FROM FECAL SAMPLES OF BALI CATTLE AT LOCAL FARM ON LOMBOK ISLAND Gunawan; Seli Nurmayani; Jayanti Mandrasari; Kunti Tirtasari; Kholik
JURNAL SANGKAREANG MATARAM Vol. 4 No. 3 (2018): September 2018
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Lombok Province has Earth of Million Cattle Program. Antibiotic resistance that caused by miss antibiotic used is a major problem in the world. The aim of this study was to the isolation of Escherichia coli and their resistance of Antibiotic from fecal samples of Bali cattle of the Local farm on Lombok Island to prevent an economic loss that caused by ineffective of treatment and to antibiotic resistance in cattle and human. An observational survey with explorative laboratory was conducted in this study. Fecal samples were collected during July 2018. A fecal sample was taken by rectal exploration. Thus fecal sample was put into Brain Infusion Heart tube media by cotton swabs and they were incubated at 37℃ for 24 h to produce colony growth. The bacterial colonies were streaked on Petri dishes containing Eosin Methylene Blue Agar and then incubated for 24 h at 37°C. The purified bacterial cultures were done using Gram staining and biochemical test. The E. coli isolated were performed by a standard National Committee for Clinical Laboratory Standards (NCCLS) disk diffusion method. The result of this study showed that 94.4% E. coli isolates were resistance of Erythromycin, 61.1% were resistance of Erythromycin of Tetracycline, and 16.7% were resistance of Ciprofloxacin of 20 E.coli were isolated.
Co-Authors . Kholik Alfiana Laili Dwi Agustin Andreyani Attamimi Erni Gunawan Jayanti Mandrasari Novarina Sulsia Ista’in Ningtyas Rima Nurmayani Seli Nurmayani