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Wuryani Wuryani
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

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ISOLASI GENISTEIN DARI TEMPE SECARA KROMATOGRAFI Wuryani Wuryani
Jurnal Kimia Terapan Indonesia Vol 4, No 1 (1994)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3916.575 KB) | DOI: 10.14203/jkti.v4i1.254

Abstract

The presence of genisteln in tempe (fermented soybean) could be detected by using various chromatographic techniques, such as Thin-Layer, Column and High Pressure Liquid Chromatography. In this experiment, TLC was chosen for the isolation and purification of genistein from tempe. Spectrophotometry, Mass Spectrometry, spraying with chromogenic reagents and hydro- lysis were carried out for identifying genistein. Quantification was carried out spectrophotometrlcally and it was shown that the laboratory prepared tempe (l00 g) contained 13.80 mg ofgenisteln.
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