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All Journal Indonesian Journal of Cancer Chemoprevention
Dita Brenna Septhea
Cancer Chemoprevention Reearch Center, Faculty of Pharmacy Universitas Gadjah Mada
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Ficus septica burm. F. Leaves Ethanolic Extract Induces Apoptosis in 7,12-dimethylbenz[a]nthracene-induced Rat Liver Cancer Quatitavely Septhea, Dita Brenna; Anindyajati, .; Darma, Andita Pra; Nurzijah, Ika; Nugroho, Agung Endro
Indonesian Journal of Cancer Chemoprevention Vol 2, No 2 (2011)
Publisher : Indonesian Research Gateway

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Abstract

The chemopreventive effect of  Ficus septica Burm. f. leaves ethanolic extract (FLEE) was studied in 7,12-dimethylbenz[a]nthracene (DMBA)-induced rat liver cancer. Rats were divided into 5 group, 5 rats (5 wk of age Sprague Dawley rat) in each group. Group 1 was control diet group, administered with 0,5% CMC-Na as vehicle. FLEE was administered 750 mg/kgBW and 1500 mg/kgBW starting 4 wk until 5 wk after DMBA administration at the first until fifth wk to group 2 and group 3. Group 4 was control extract group, administered  with 750 mg/kgBW and  group  5  was  DMBA  group.  DMBA  is  a  carcinogen  to  induce  liver  cancer  was  also administered in DMBA control group and all animals were necropsied at 6 wk after DMBA administration. Activity of inducing apoptosis was detected using Double Staining method in 750 mg/kgBW FLEE group compared to control group but no in 1500 mg/kgBW FLEE group resulted in 100% dead. Apoptotic cells would have orange flourescence but normal cells would have green flourescence detected by flourescence microscope. To investigate the protein that involved in apoptotic mechanism, we studied p53 expression using Imunohistochemistry (IHC). There was no difference expression of p53 in both tested and control groups. Based on the results, FLEE has a potency as chemoprentive agent because its activity on inducing apoptosis in liver  cancer  with  p53-independent  pathway.  The  mechanism  of  apoptosis  induction  of  this extract needs to be explored by observing the expression of related proteins. Key words: apoptosis, Ficus septica, liver cancer, p53 independent pathway
Ficus septica Burm.f. Leaves Ethanolic Extract Triggered Apoptosis on 7,12-Dimethylbenz[a]anthracene-Induced Rat Mammary Carcinogenesis Qualitatively Anindyajati, .; Darma, Andita Pra; Nurzijah, Ika; Septhea, Dita Brenna; Nugroho, Agung Endro
Indonesian Journal of Cancer Chemoprevention Vol 3, No 1 (2012)
Publisher : Indonesian Research Gateway

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Abstract

Ficus septica Burm.f.ethanolic extract (FEE) shows cytotoxic effects on several cancer cell lines. Our research aimed to investigate the effect of FEE on apoptosis induction and p53 expression against carcinogenesis of 7,12-Dimenthylbenz[a]anthracene (DMBA)-induced rat mammary. The research was conducted by comparing both apoptosis induction and p53 expression in DMBA-induced rats that were treated with FEE against control groups. Cells that undergo apoptosis were visualized by Double Staining method with acridine orange and ethidium bromide, while p53 expression was detected by IHC staining. Double staining result showed increased occurrence of apoptotic cells compared to the control groups. IHC staining pf P53 did not show significant difference between treatment and control groups. However, FEE was able to repair morphology of cells undergoing carciogenesis. Thus, we conclude that FEE has an anti carciogenic activity on DMBA-induced rat mammary through apoptosis induction without affecting p53 expression. Therefore, the ethanolic extract of Ficus septica leaves is a potential chemo-preventive agent on breast cancer. Further study on its molecular mechanism needs to be exploredKeywords: Ficus septica, breast cancer, 7,12-Dimethylbenz[a]anthracene, carciogenesis, apoptosis, p53
Co-Authors . Anindyajati Agung Endro Nugroho Andita Pra Darma Ika Nurzijah