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All Journal Caraka Tani: Journal of Sustainable Agriculture AgriHealth: Journal of Agri-food, Nutrition and Public Health
Rendie Prasetyo
Department of Botany, Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto
Agriculture, Biological Sciences & Forestry Education Environmental Science Health Professions Public Health

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Media Manipulation to Enhance In Vitro Pitcher Formation in Nepenthes mirabilis (Lour.) Druce for Microfloriculture Development Sugiyono Sugiyono; Anindya Puspaning Tyas; Dea Yuniar Indikasuri; Sri Lestari; Paul A Davison; Rendie Prasetyo
Caraka Tani: Journal of Sustainable Agriculture Vol 40, No 3 (2025): July
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v40i3.101139

Abstract

Microfloriculture refers to souvenirs and Living Room Ornaments made from the in vitro cultured ornamental plants, which are innovative, unique, environmentally friendly, and can be produced across the year. In vitro pitcher-forming Nepenthes mirabilis will increase the attractiveness and market value of microfloriculture products. Two experiments have been carried out to stimulate in vitro pitcher formation in N. mirabilis using a completely randomized design (CRD) of a two-factorial treatment pattern with three replicates. The first experiment tested different Murashige and Skoog (MS) media formulations (full-strength, 3/4MS, 1/2MS, and 1/4MS) and phytagel concentrations (0, 2.5, 3.5, and 4.5 g l-1). The same media formulations and 6-Benzylaminopurine (BAP) concentrations (0, 0.5, 1.0, and 1.5 μM) were tested in the second experiment. The results showed that the explants cultured on 1/4MS media produced the greatest number of shoots (3.83-8.25) and leaves (26.17-26.75), the earliest pitcher formation (27.75±2.06 days), the highest number of pitchers (6.50±2.65), and pitcher-forming leaves (23.30±5.10%). Liquid media enhanced leaves (28.33±4.97) and pitchers (6.42±3.36) formation as well as pitcher-forming leaves percentage (21.37±8.01%). A 1/4MS supplemented with 0.5 μM BAP maximized pitcher-forming leaves (21.08±7.78%) and pitcher number (5.67±2.52). Adding 1.5 μM BAP on 1/4MS media produced the highest number of leaves (38.67±5.69). Full-strength MS produced the lowest number of shoots, leaves, pitchers, and pitcher-forming leaves. This study developed an affordable and effective method to improve in vitro pitcher development in N. mirabilis, highlighting how nutrient levels and BAP concentration influence pitcher formation, which aids in optimizing culture techniques for ornamental carnivorous plants.
Modification of Media Formulation and Agar Concentration to Improve Pitcher Plant (Nepenthes mirabilis (Lour.) Druce) Micropropagation for Conservation and Microfloriculture Development Andes Khofifah Putri; Rendie Prasetyo; Elly Proklamasiningsih; Paul A. Davison; Sugiyono Sugiyono
AgriHealth: Journal of Agri-food, Nutrition and Public Health Vol 5, No 1 (2024): April
Publisher : Research and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrihealth.v5i1.85361

Abstract

The pitcher plant (Nepenthes mirabilis (Lour.) Druce) is a unique plant listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendix II and is protected in Indonesia. Conventional propagation of N. mirabilis is difficult and takes a longer time. Therefore, in vitro culture method is proposed. This study aimed to determine the best and most economical media formulation and agar concentration for N. mirabilis micropropagation. This research has been carried out experimentally using a completely randomized two-factor factorial design. The first factor was the media formulation (full-strength Murashige and Skoog (MS), half-strength MS, half-strength MS + AB mix, and AB mix) and the second factor was agar concentrations (6, 8, and 10 g l-1). Twelve treatment combinations were obtained and repeated 5 times to produce 60 experimental units. The explants were apical microshoots (1.5 cm long with 5 leaflets). The cultures were incubated at 24 ℃ under continuous light for 16 weeks. The parameters measured included shoot emergence time, number of shoots, number of leaves, and shoot length. The data were analyzed using variance analysis followed by Duncan’s multiple range test at a 95% confidence level. The results showed that half-strength MS medium resulted in the highest number of shoots and leaves and the longest shoot length, whereas adding 8 g l-1 agar resulted in the fastest shoot emergence time. Half-strength MS medium solidified with 8 g l-1 agar could produce many N. mirabilis (Lour.) Druce microshoots to support both conservation and microfloriculture development.
Co-Authors Andes Khofifah Putri Anindya Puspaning Tyas Dea Yuniar Indikasuri Elly Proklamasiningsih Erik Harry Murchie Ilham Warfa’ni Khavid Faozi Lucky Prayoga Paul A Davison Paul A. Davison Prita Sari Dewi Sri Lestari Sugiyono Sugiyono Sugiyono Sugiyono