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All Journal Caraka Tani: Journal of Sustainable Agriculture AgriHealth: Journal of Agri-food, Nutrition and Public Health
Rendie Prasetyo
Department of Botany, Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto
Agriculture, Biological Sciences & Forestry Education Environmental Science Health Professions Public Health

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Banana Cultivars Microshoot Induction and Plantlet Formation Using Cytokinin and Auxin Sugiyono Sugiyono; Prita Sari Dewi; Rendie Prasetyo
Caraka Tani: Journal of Sustainable Agriculture Vol 36, No 2 (2021): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v36i2.50425

Abstract

Banana is a horticultural plant with very high potentials, which contains carbohydrates and vitamins that are useful in fulfilling people's food and nutritional needs. Hence, this study aims to produce superior banana seedlings and develop a protocol for their mass production using a plant in vitro culture technique. This was a two stage-experiment i.e. microshoot production and plantlet formation. The result showed that Gebyar cultivar produced more shoots than the Kepok Kuning cultivar, with an average of 4.25 microshoots explant-1. However, Kepok Kuning produced more leaves than Gebyar, with an average of 4.64 leaves plantlet-1. Banana shoots cultured on the media containing Indole-3-acetic acid (IAA) at a concentration of 2.5 µM produced the highest leaves number. Meanwhile, those cultured on the media containing 1-Naphthalenesacetic acid (NAA) at a concentration of 7.5 µM produced the highest roots number. A Murashige and Skoog (MS) medium supplemented with 6-Benzylaminopurine (BAP) up to 30 µM and the one supplemented with 7.5 μM of NAA are suitable for Kepok Kuning and Gebyar cultivars micropropagation with regard to microshoot induction and plantlet formation, respectively.
The Effect of Media Types and NAA Concentrations on Agarwood (Aquilaria malaccensis Lamk.) Shoot Development in In Vitro Culture Ilham Warfa’ni; Lucky Prayoga; Rendie Prasetyo; Erik Harry Murchie; Sugiyono Sugiyono
AgriHealth: Journal of Agri-food, Nutrition and Public Health Vol 3, No 1 (2022): April
Publisher : Research and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrihealth.v3i1.60346

Abstract

Agarwood (Aquilaria malaccensis Lamk.) is a forest product commodity with high economic value, but its availability in nature is decreasing due to deforestation and overexploitation. The availability of high-quality seedlings is necessary to meet the demand and conserve the sources of agarwood plants. Therefore, this study investigates the interaction effect between media types and 1-Naphthaleneacetic acid (NAA) concentrations on agarwood shoot development. It also aims to determine the best medium type and NAA concentration to stimulate agarwood shoot development. This research had two stages, namely the shoot induction and development. The shoot induction stage was conducted experimentally, using a completely randomized design (CRD) on a factorial treatment pattern of 2 factors i.e., the types of media and NAA concentrations. The shoots produced from the induction stage were cultured on Murashige-Skoog (MS) media with the same media types but without any NAA addition. Furthermore, the variables observed in both stages were the development of agarwood shoots, and the parameters measured included the number of shoots, number of leaves and shoot lengths. The results showed that the development of agarwood shoots was controlled by the type of medium used. Liquid MS medium supported with filter paper bridge produced the highest average number of shoots with 4.93 and 5.87 shoots explants-1 and length at 3.25 and 3.64 cm explant-1 in the induction and development stage, respectively. These findings will facilitate mass propagation of agarwood shoots, and in turn the availability of agarwood plantlets supports its conservation and production.
Effects of Media and Plant Growth Regulators on Mini-Tuber Yield of Granola Cultivar of Potato in Aeroponics System Sugiyono Sugiyono; Lucky Prayoga; Elly Proklamasiningsih; Khavid Faozi; Rendie Prasetyo
AgriHealth: Journal of Agri-food, Nutrition and Public Health Vol 2, No 1 (2021): April
Publisher : Research and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrihealth.v2i1.53197

Abstract

Potato is a perennial plant (Solanum tuberosum L.) in the nightshade family, which is one of the most developed and competitive vegetable crops compared to other vegetables. Furthermore, its production could not meet the increasing demand, which led to very intensive research and development programs. This research aims to examine the effect of nutrients and growth regulators on the yield of mini-tuber in the aeroponic system and to determine the best type of nutrient, as well as the type and concentration of plant growth regulators to increase mini-tubers yield in an aeroponics system. This experiment has been carried out experimentally using a split-split plot design. The main plots were the types of nutrient media, which consisted of Grow More-modified nutrient and AB-Mix nutrient. The sub-plots were the types of plant growth regulator (PGR), which consisted of BAP and GA3, while the sub-sub-plot were the concentrations of PGR, which consisted of 0; 5; 10; 15; 20 and 25 µM. Each combination of treatments was repeated three times. The results showed that the use of media type, PGR and PGR concentration affected the formation and yield of Granola cultivar of potato mini-tubers. Knols grown in AB-Mix nutrient medium supplemented with 20 µM GA3 resulted in the best mini-tuber formation, as shown by the average number of mini-tubers of 8.33, the total weight of mini-tubers of 21.38 g and the average mini-tuber diameter of 13.05 mm.
Modification of Media Formulation and Agar Concentration to Improve Pitcher Plant (Nepenthes mirabilis (Lour.) Druce) Micropropagation for Conservation and Microfloriculture Development Andes Khofifah Putri; Rendie Prasetyo; Elly Proklamasiningsih; Paul A. Davison; Sugiyono Sugiyono
AgriHealth: Journal of Agri-food, Nutrition and Public Health Vol 5, No 1 (2024): April
Publisher : Research and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrihealth.v5i1.85361

Abstract

The pitcher plant (Nepenthes mirabilis (Lour.) Druce) is a unique plant listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendix II and is protected in Indonesia. Conventional propagation of N. mirabilis is difficult and takes a longer time. Therefore, in vitro culture method is proposed. This study aimed to determine the best and most economical media formulation and agar concentration for N. mirabilis micropropagation. This research has been carried out experimentally using a completely randomized two-factor factorial design. The first factor was the media formulation (full-strength Murashige and Skoog (MS), half-strength MS, half-strength MS + AB mix, and AB mix) and the second factor was agar concentrations (6, 8, and 10 g l-1). Twelve treatment combinations were obtained and repeated 5 times to produce 60 experimental units. The explants were apical microshoots (1.5 cm long with 5 leaflets). The cultures were incubated at 24 ℃ under continuous light for 16 weeks. The parameters measured included shoot emergence time, number of shoots, number of leaves, and shoot length. The data were analyzed using variance analysis followed by Duncan’s multiple range test at a 95% confidence level. The results showed that half-strength MS medium resulted in the highest number of shoots and leaves and the longest shoot length, whereas adding 8 g l-1 agar resulted in the fastest shoot emergence time. Half-strength MS medium solidified with 8 g l-1 agar could produce many N. mirabilis (Lour.) Druce microshoots to support both conservation and microfloriculture development.
Co-Authors Andes Khofifah Putri Elly Proklamasiningsih Erik Harry Murchie Ilham Warfa’ni Khavid Faozi Lucky Prayoga Paul A. Davison Prita Sari Dewi Sugiyono Sugiyono Sugiyono Sugiyono