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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology
M A Setiadi
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Agriculture, Biological Sciences & Forestry

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Characteristics and in Vitro Fertilization Ability of Ram Spermatozoa: Comparison of Epididymal and Ejaculated Spermatozoa F Pamungkas; M A Setiadi; N W.K Karja
Media Peternakan Vol. 35 No. 1 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2991.621 KB) | DOI: 10.5398/medpet.2012.35.1.38

Abstract

The characteristics and in vitro fertilization (IVF) ability of ram spermatozoa collected from cauda epididymal was examined. Ejaculated spermatozoa was used as control group in this experiment. Characteristics of spermatozoa including the percentage of progressive motility, viability, abnormality and membrane integrity were evaluated before and after freezing. Fertilization ability of post-thawed spermatozoa in both group was examined based on the pronucleus formation after IVF of in vitro matured (IVM) oocytes. Results from these study showed that there were no significant differences in the characteristics between cauda epididymal and ejaculated spermatozoa before freezing. After freezing, motility of ejaculated spermatozoa was higher than epididymal spermatozoa (54.00±2.24% vs 48.00±4.47%), however the membrane integrity of epididymal spermatozoa was higher than ejaculated spermatozoa (75.38±9.32% vs 65.54±11.88%) (P experiment revealed that the ability of post-thawed epididymal spermatozoa to fertilize oocytes (61.40%, 42.98%, 18.42% for total, normal and polysperm, respectively) did not differ from that of ejaculated spermatozoa (66.67%, 48.78%, 17.89% for total, normal and polysperm, respectively). These results indicate that ram spermatozoa collected from cauda epididymal and then frozen have the ability to fertilize ram ooctyes in vitro in the similar rate with ejaculated spermatozoa.
In Vitro Fertility of Post-thawed Epididymal Ram Spermatozoa after Storage at 5 °C before Cryopreservation N W.K Karja; M fahrudin; M A Setiadi
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (430.039 KB) | DOI: 10.5398/medpet.2013.36.1.26

Abstract

This study addressed the effects of storage duration of epididymides at 5 °C before sperm collection and their fertility after cryopreservation in vitro. Spermatozoa from one of the testes pairs were immediately collected, evaluated and frozen (control group). The remaining epididymides were cooled to 5 °C and stored for 24, 48, 72, and 96 h (experimental groups), after which spermatozoa were collected and frozen as in the control group. Before and after thawing, sperm motility, sperm viability and plasma membrane integrity were assessed. The fertilizing ability of frozen-thawed spermatozoa of each group was evaluated by in vitro fertilization of matured sheep oocytes. Sperm quality (sperm motility, viability, and plasma membrane integrity) at collection and after cryopreservation decreased as the duration of the epididymal storage interval increase (P < 0.05). The motility decreased steadily along the studied time periods. Although, the fertilizing ability of post-thawed epididymal spermatozoa gradually decreased as the storage period was prolonged, the spermatozoa collected from the cauda epididymides stored at 5 °C for up to 96 h were able to fertilize 16%-65% of oocytes in vitro. Results of the present study showed that ram epididymal spermatozoa survive in storage at 5 °C for up to 96 h. These spermatozoa maintain their fertilizing ability and may be suitable for use in IVF and other assisted reproductive procedures.
Co-Authors F Pamungkas M fahrudin N W.K Karja