Perbedaan manajemen asisten dosen laboratorium pada berbagai jensi laboratorium disebabkan oleh adanya perbedaan kebijakan setiap kepala laboratorium. Untuk itu, dilakukan kajian mengenai problematika dan standarisasi asisten laboratorium di perguruan tinggi, utamanya prodi pendidikan sains pada jenjang sarjana. Penelitian dilakukan menggunakan pendekatan deskriptif kualitatif untuk menganalisis input, proses dan output dalam Perguruan Tinggi. Data kualitatif diperoleh melalui kegiatan wawancara kepada asisten dosen di beberapa perguruan tinggi seperti Universitas Negeri Malang, Universitas Negeri Jember dan Universitas Sebelas Maret. Wawancara berisi pertanyaan terkait proses perekrutan, kegiatan dalam laboratorium saat praktikum dan setelah praktikum serta reward yang didapatkan setelah menjadi asisten dosen laboratorium. Hasil yang diperoleh bahwa manajemen laboratorium mengenai asisten laboratorium dilapangan menemui beberapa permasalahan seperti tidak ada standarisasi terkait syarat/kualifikasi perekrutan asisten laboratorium, tidak ada honorarium, dan mahasiswa tidak mendapat sertifikasi setelah selesai melaksanakan tugas. Selain itu, belum terdapat standarisasi manajemen asisten dosen laboratorium dalam skala nasional.Differences in the management of laboratory assistant lecturers in various types of laboratories are caused by differences in the policies of each laboratory head. For this reason, a study was carried out on the problems and standardization of laboratory assistants in universities, especially science education study programs at the undergraduate level. The research was conducted using a qualitative descriptive approach to analyze input, process and output in higher education. Qualitative data were obtained through interviews with teaching assistants at several universities such as Malang State University, Jember State University and Sebelas Maret University. The interview contains questions related to the recruitment process, activities in the laboratory during practicum and after practicum as well as rewards obtained after becoming a laboratory lecturer assistant. The results obtained are that laboratory management regarding laboratory assistants in the field encounters several problems such as there is no standardization regarding the requirements/qualifications for the recruitment of laboratory assistants, there is no honorarium, and students do not receive certification after completing their assignments. In addition, there is no standardized management of laboratory assistant lecturers on a national scale.