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D. Setiyaningsih
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Molecular characterization of Bovine herpesvirus type 1 Indonesian isolates Saepulloh, Muharam; Wibawan, I.W.T.; Sajuthi, D.; Setiyaningsih, D.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 1 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.846 KB) | DOI: 10.14334/jitv.v14i1.365

Abstract

Different subtypes of bovine herpesvirus 1 (BHV-1) have been associated with different clinical conditions of cattle. For that reason subtypes differentiation has become an essential tool for understanding the pathogenesis and epidemiology of BHV infections. In search for a genomic region that would allow a clear distinction between BHV-1.1 and BHV-1.2 of glycoprotein D (gD) genes of 8 Indonesian isolates were amplified and sequenced. The amino acid sequence alignments revealed that the levels of genomic similarity ranging from 98.8 to 100% among BHV-1 Indonesian isolates and its results were also similar between BHV-1 Indonesia isolates and BHV-1.1 reference, and 98.4 to 98.8% between BHV-1 Indonesian isolates and BHV-1.2 reference. The isolates could be clearly separated into BHV-1.1 and BHV-1.2 after phylogenetic analysis. The results showed that the Indonesian isolates were characterized as BHV-1.1 as agent caused respiratory tract infections in cattle or infectious bovine rhinotracheitis (IBR) disease. The results suggest that the phylogenetic analysis performed here can be used as a potential molecular epidemiological tool for herpesviruses. Key Words: BHV-1.1, BHV-1.2, Glycoprotein D, Phylogenetic Analysis, IBR
Molecular characterization of Bovine herpesvirus type 1 Indonesian isolates Muharam Saepulloh; I.W.T. Wibawan; D. Sajuthi; D. Setiyaningsih
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 1 (2009): MARCH 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.846 KB) | DOI: 10.14334/jitv.v14i1.365

Abstract

Different subtypes of bovine herpesvirus 1 (BHV-1) have been associated with different clinical conditions of cattle. For that reason subtypes differentiation has become an essential tool for understanding the pathogenesis and epidemiology of BHV infections. In search for a genomic region that would allow a clear distinction between BHV-1.1 and BHV-1.2 of glycoprotein D (gD) genes of 8 Indonesian isolates were amplified and sequenced. The amino acid sequence alignments revealed that the levels of genomic similarity ranging from 98.8 to 100% among BHV-1 Indonesian isolates and its results were also similar between BHV-1 Indonesia isolates and BHV-1.1 reference, and 98.4 to 98.8% between BHV-1 Indonesian isolates and BHV-1.2 reference. The isolates could be clearly separated into BHV-1.1 and BHV-1.2 after phylogenetic analysis. The results showed that the Indonesian isolates were characterized as BHV-1.1 as agent caused respiratory tract infections in cattle or infectious bovine rhinotracheitis (IBR) disease. The results suggest that the phylogenetic analysis performed here can be used as a potential molecular epidemiological tool for herpesviruses. Key Words: BHV-1.1, BHV-1.2, Glycoprotein D, Phylogenetic Analysis, IBR
Co-Authors D. Sajuthi D. Sajuthi I.W.T. Wibawan I.W.T. Wibawan Muharam Saepulloh Muharam Saepulloh